Quantitative determination of telavancin in pregnant baboon plasma by solid-phase extraction and LC-ESI-MS

Xiao-Ming Wang, Jonathan A. Paul, Tatiana Nanovskaya, Gary Hankins, Mahmoud Ahmed

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The increasing incidence and severity of methicillin- and vancomycin-resistant infections during pregnancy prompted further development of telavancin. The understanding of the pharmacokinetics of telavancin during pregnancy is critical to optimize dosing. Due to ethical and safety concerns the study is conducted on the pregnant baboons. A method using solid-phase extraction coupled with liquid chromatography-single quadrupole mass spectrometry for the quantitative determination of telavancin in baboon plasma samples was developed and validated. Teicoplanin was used as an internal standard. Telavancin was extracted from baboon plasma samples by using Waters Oasis® MAX 96-Well SPE plate and achieved extraction recovery was >66% with variation <12%. Telavancin was separated on Waters Symmetry C18 column with gradient elution. Two SIM channels were monitored at m/z 823 and m/z 586 to achieve quantification with simultaneous confirmation of telavancin identification in baboon plasma samples. The linearity was assessed in the range of 0.188μg/mL to75.0μg/mL, with a correlation coefficient of 0.998. The relative standard deviation of this method was <11% for within- and between-run assays, and the accuracy ranged between 96% and 114%.

Original languageEnglish (US)
Pages (from-to)107-112
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume98
DOIs
StatePublished - 2014

Fingerprint

Papio
Solid Phase Extraction
Plasmas
Teicoplanin
Pregnancy
Methicillin Resistance
Methicillin
Pharmacokinetics
Water
Liquid chromatography
Vancomycin
Liquid Chromatography
Mass spectrometry
telavancin
Assays
Mass Spectrometry
Safety
Recovery
Incidence
Infection

Keywords

  • Baboon plasma
  • LC-ESI-MS
  • SPE
  • Telavancin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry

Cite this

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title = "Quantitative determination of telavancin in pregnant baboon plasma by solid-phase extraction and LC-ESI-MS",
abstract = "The increasing incidence and severity of methicillin- and vancomycin-resistant infections during pregnancy prompted further development of telavancin. The understanding of the pharmacokinetics of telavancin during pregnancy is critical to optimize dosing. Due to ethical and safety concerns the study is conducted on the pregnant baboons. A method using solid-phase extraction coupled with liquid chromatography-single quadrupole mass spectrometry for the quantitative determination of telavancin in baboon plasma samples was developed and validated. Teicoplanin was used as an internal standard. Telavancin was extracted from baboon plasma samples by using Waters Oasis{\circledR} MAX 96-Well SPE plate and achieved extraction recovery was >66{\%} with variation <12{\%}. Telavancin was separated on Waters Symmetry C18 column with gradient elution. Two SIM channels were monitored at m/z 823 and m/z 586 to achieve quantification with simultaneous confirmation of telavancin identification in baboon plasma samples. The linearity was assessed in the range of 0.188μg/mL to75.0μg/mL, with a correlation coefficient of 0.998. The relative standard deviation of this method was <11{\%} for within- and between-run assays, and the accuracy ranged between 96{\%} and 114{\%}.",
keywords = "Baboon plasma, LC-ESI-MS, SPE, Telavancin",
author = "Xiao-Ming Wang and Paul, {Jonathan A.} and Tatiana Nanovskaya and Gary Hankins and Mahmoud Ahmed",
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T1 - Quantitative determination of telavancin in pregnant baboon plasma by solid-phase extraction and LC-ESI-MS

AU - Wang, Xiao-Ming

AU - Paul, Jonathan A.

AU - Nanovskaya, Tatiana

AU - Hankins, Gary

AU - Ahmed, Mahmoud

PY - 2014

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AB - The increasing incidence and severity of methicillin- and vancomycin-resistant infections during pregnancy prompted further development of telavancin. The understanding of the pharmacokinetics of telavancin during pregnancy is critical to optimize dosing. Due to ethical and safety concerns the study is conducted on the pregnant baboons. A method using solid-phase extraction coupled with liquid chromatography-single quadrupole mass spectrometry for the quantitative determination of telavancin in baboon plasma samples was developed and validated. Teicoplanin was used as an internal standard. Telavancin was extracted from baboon plasma samples by using Waters Oasis® MAX 96-Well SPE plate and achieved extraction recovery was >66% with variation <12%. Telavancin was separated on Waters Symmetry C18 column with gradient elution. Two SIM channels were monitored at m/z 823 and m/z 586 to achieve quantification with simultaneous confirmation of telavancin identification in baboon plasma samples. The linearity was assessed in the range of 0.188μg/mL to75.0μg/mL, with a correlation coefficient of 0.998. The relative standard deviation of this method was <11% for within- and between-run assays, and the accuracy ranged between 96% and 114%.

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