Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose

Mitchell R. Smith; Catherine S. Devine; Steven M. Cohn; Michael W. Lieberman

doi:10.1016/0003-2697(84)90356-7

Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose

Mitchell R. Smith, Catherine S. Devine, Steven M. Cohn, Michael W. Lieberman

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp.

Original language	English (US)
Pages (from-to)	120-124
Number of pages	5
Journal	Analytical Biochemistry
Volume	137
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(84)90356-7
State	Published - Feb 15 1984
Externally published	Yes

Keywords

DNA
agarose gel electrophoresis
electrophoretic transfer
hybridization
nitrocellulose
polyacrylamide gel electrophoresis

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(84)90356-7

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title = "Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose",

abstract = "A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp.",

keywords = "DNA, agarose gel electrophoresis, electrophoretic transfer, hybridization, nitrocellulose, polyacrylamide gel electrophoresis",

author = "Smith, {Mitchell R.} and Devine, {Catherine S.} and Cohn, {Steven M.} and Lieberman, {Michael W.}",

note = "Funding Information: This work was supported by NIH Grants CA205 13 and CA3 1734. and by the following companies: Brown & Wil-liamson Tobacco Corporation, Philip Morris Incorporated, R. J. Reynolds Tobacco Company, and United States Tobacco Company. M.R.S. was a fellow of the Environmental Pathology Program of Washington University (ES07066).",

year = "1984",

month = feb,

day = "15",

doi = "10.1016/0003-2697(84)90356-7",

language = "English (US)",

volume = "137",

pages = "120--124",

journal = "Analytical Biochemistry",

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publisher = "Academic Press Inc.",

number = "1",

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TY - JOUR

T1 - Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose

AU - Smith, Mitchell R.

AU - Devine, Catherine S.

AU - Cohn, Steven M.

AU - Lieberman, Michael W.

N1 - Funding Information: This work was supported by NIH Grants CA205 13 and CA3 1734. and by the following companies: Brown & Wil-liamson Tobacco Corporation, Philip Morris Incorporated, R. J. Reynolds Tobacco Company, and United States Tobacco Company. M.R.S. was a fellow of the Environmental Pathology Program of Washington University (ES07066).

PY - 1984/2/15

Y1 - 1984/2/15

N2 - A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp.

AB - A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp.

KW - DNA

KW - agarose gel electrophoresis

KW - electrophoretic transfer

KW - hybridization

KW - nitrocellulose

KW - polyacrylamide gel electrophoresis

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DO - 10.1016/0003-2697(84)90356-7

M3 - Article

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AN - SCOPUS:0021382823

SN - 0003-2697

VL - 137

SP - 120

EP - 124

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose

Abstract

Keywords

ASJC Scopus subject areas

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