Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose

Mitchell R. Smith, Catherine S. Devine, Steven Cohn, Michael W. Lieberman

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp.

Original languageEnglish (US)
Pages (from-to)120-124
Number of pages5
JournalAnalytical Biochemistry
Volume137
Issue number1
DOIs
StatePublished - Feb 15 1984
Externally publishedYes

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Keywords

  • agarose gel electrophoresis
  • DNA
  • electrophoretic transfer
  • hybridization
  • nitrocellulose
  • polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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