Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose

Mitchell R. Smith; Catherine S. Devine; Steven Cohn; Michael W. Lieberman

doi:10.1016/0003-2697(84)90356-7

Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose

Mitchell R. Smith, Catherine S. Devine, Steven Cohn, Michael W. Lieberman

Research output: Contribution to journal › Article

13 Scopus citations

Abstract

A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp.

Original language	English (US)
Pages (from-to)	120-124
Number of pages	5
Journal	Analytical Biochemistry
Volume	137
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(84)90356-7
State	Published - Feb 15 1984
Externally published	Yes

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Keywords

agarose gel electrophoresis
DNA
electrophoretic transfer
hybridization
nitrocellulose
polyacrylamide gel electrophoresis

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Cite this

Smith, M. R., Devine, C. S., Cohn, S., & Lieberman, M. W. (1984). Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose. Analytical Biochemistry, 137(1), 120-124. https://doi.org/10.1016/0003-2697(84)90356-7

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abstract = "A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp.",

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AU - Smith, Mitchell R.

AU - Devine, Catherine S.

AU - Cohn, Steven

AU - Lieberman, Michael W.

PY - 1984/2/15

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N2 - A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp.

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KW - agarose gel electrophoresis

KW - DNA

KW - electrophoretic transfer

KW - hybridization

KW - nitrocellulose

KW - polyacrylamide gel electrophoresis

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10.1016/0003-2697(84)90356-7