Abstract
A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 120-124 |
| Number of pages | 5 |
| Journal | Analytical Biochemistry |
| Volume | 137 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 15 1984 |
| Externally published | Yes |
Keywords
- agarose gel electrophoresis
- DNA
- electrophoretic transfer
- hybridization
- nitrocellulose
- polyacrylamide gel electrophoresis
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Cite this
Smith, M. R., Devine, C. S., Cohn, S., & Lieberman, M. W. (1984). Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose. Analytical Biochemistry, 137(1), 120-124. https://doi.org/10.1016/0003-2697(84)90356-7