Abstract
A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 120-124 |
| Number of pages | 5 |
| Journal | Analytical Biochemistry |
| Volume | 137 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 15 1984 |
| Externally published | Yes |
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Keywords
- agarose gel electrophoresis
- DNA
- electrophoretic transfer
- hybridization
- nitrocellulose
- polyacrylamide gel electrophoresis
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose. / Smith, Mitchell R.; Devine, Catherine S.; Cohn, Steven; Lieberman, Michael W.
In: Analytical Biochemistry, Vol. 137, No. 1, 15.02.1984, p. 120-124.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose
AU - Smith, Mitchell R.
AU - Devine, Catherine S.
AU - Cohn, Steven
AU - Lieberman, Michael W.
PY - 1984/2/15
Y1 - 1984/2/15
N2 - A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp.
AB - A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp.
KW - agarose gel electrophoresis
KW - DNA
KW - electrophoretic transfer
KW - hybridization
KW - nitrocellulose
KW - polyacrylamide gel electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=0021382823&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021382823&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(84)90356-7
DO - 10.1016/0003-2697(84)90356-7
M3 - Article
C2 - 6731793
AN - SCOPUS:0021382823
VL - 137
SP - 120
EP - 124
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -