QUANTITATIVE GAS CHROMATOGRAPHIC ANALYSIS OF AMINO ACIDS IN BEER AND WORT

Alexander Kurosky, Austra Bars

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Amino acids present in beer and wort samples were isolated using Dowex 50W‐X8 ion exchange resin. These acids were converted to N‐trifluoroacetyl methyl esters and were separated on a 10 ft. x 1/4 in. copper column packed with 3/4% Carbowax 1540 and 1/4% NPGS on Chromosorb W. Complete separation and quantitative estimation was obtained for eleven amino acids: valine, alanine, isoleucine, leucine, glycine, proline, aspartic acid, threonine, methionine, glutamic acid, and phenylalanine. Standards of the acids showed a relative standard deviation of 4% and less for nine acids while threonine and methionine gave deviations of 9% and 15% respectively. The other naturally‐occurring amino acids did not interfere with the separation of the above eleven. Amino acid assimilation data is presented along with other beer and wort analyses. 1968 The Institute of Brewing & Distilling

Original languageEnglish (US)
Pages (from-to)200-207
Number of pages8
JournalJournal of the Institute of Brewing
Volume74
Issue number2
DOIs
StatePublished - 1968
Externally publishedYes

Fingerprint

wort (brewing)
beers
Gas Chromatography
gas chromatography
Amino Acids
amino acids
Threonine
threonine
Methionine
Acids
methionine
acids
Ion Exchange Resins
ion exchange resins
Isoleucine
brewing
Valine
aspartic acid
isoleucine
valine

ASJC Scopus subject areas

  • Food Science

Cite this

QUANTITATIVE GAS CHROMATOGRAPHIC ANALYSIS OF AMINO ACIDS IN BEER AND WORT. / Kurosky, Alexander; Bars, Austra.

In: Journal of the Institute of Brewing, Vol. 74, No. 2, 1968, p. 200-207.

Research output: Contribution to journalArticle

Kurosky, Alexander ; Bars, Austra. / QUANTITATIVE GAS CHROMATOGRAPHIC ANALYSIS OF AMINO ACIDS IN BEER AND WORT. In: Journal of the Institute of Brewing. 1968 ; Vol. 74, No. 2. pp. 200-207.
@article{eb42ded33db74c1d867171ee5bd57c9e,
title = "QUANTITATIVE GAS CHROMATOGRAPHIC ANALYSIS OF AMINO ACIDS IN BEER AND WORT",
abstract = "Amino acids present in beer and wort samples were isolated using Dowex 50W‐X8 ion exchange resin. These acids were converted to N‐trifluoroacetyl methyl esters and were separated on a 10 ft. x 1/4 in. copper column packed with 3/4{\%} Carbowax 1540 and 1/4{\%} NPGS on Chromosorb W. Complete separation and quantitative estimation was obtained for eleven amino acids: valine, alanine, isoleucine, leucine, glycine, proline, aspartic acid, threonine, methionine, glutamic acid, and phenylalanine. Standards of the acids showed a relative standard deviation of 4{\%} and less for nine acids while threonine and methionine gave deviations of 9{\%} and 15{\%} respectively. The other naturally‐occurring amino acids did not interfere with the separation of the above eleven. Amino acid assimilation data is presented along with other beer and wort analyses. 1968 The Institute of Brewing & Distilling",
author = "Alexander Kurosky and Austra Bars",
year = "1968",
doi = "10.1002/j.2050-0416.1968.tb03116.x",
language = "English (US)",
volume = "74",
pages = "200--207",
journal = "Journal of the Institute of Brewing",
issn = "0046-9750",
publisher = "Institute of Brewing and Distilling",
number = "2",

}

TY - JOUR

T1 - QUANTITATIVE GAS CHROMATOGRAPHIC ANALYSIS OF AMINO ACIDS IN BEER AND WORT

AU - Kurosky, Alexander

AU - Bars, Austra

PY - 1968

Y1 - 1968

N2 - Amino acids present in beer and wort samples were isolated using Dowex 50W‐X8 ion exchange resin. These acids were converted to N‐trifluoroacetyl methyl esters and were separated on a 10 ft. x 1/4 in. copper column packed with 3/4% Carbowax 1540 and 1/4% NPGS on Chromosorb W. Complete separation and quantitative estimation was obtained for eleven amino acids: valine, alanine, isoleucine, leucine, glycine, proline, aspartic acid, threonine, methionine, glutamic acid, and phenylalanine. Standards of the acids showed a relative standard deviation of 4% and less for nine acids while threonine and methionine gave deviations of 9% and 15% respectively. The other naturally‐occurring amino acids did not interfere with the separation of the above eleven. Amino acid assimilation data is presented along with other beer and wort analyses. 1968 The Institute of Brewing & Distilling

AB - Amino acids present in beer and wort samples were isolated using Dowex 50W‐X8 ion exchange resin. These acids were converted to N‐trifluoroacetyl methyl esters and were separated on a 10 ft. x 1/4 in. copper column packed with 3/4% Carbowax 1540 and 1/4% NPGS on Chromosorb W. Complete separation and quantitative estimation was obtained for eleven amino acids: valine, alanine, isoleucine, leucine, glycine, proline, aspartic acid, threonine, methionine, glutamic acid, and phenylalanine. Standards of the acids showed a relative standard deviation of 4% and less for nine acids while threonine and methionine gave deviations of 9% and 15% respectively. The other naturally‐occurring amino acids did not interfere with the separation of the above eleven. Amino acid assimilation data is presented along with other beer and wort analyses. 1968 The Institute of Brewing & Distilling

UR - http://www.scopus.com/inward/record.url?scp=84979189595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84979189595&partnerID=8YFLogxK

U2 - 10.1002/j.2050-0416.1968.tb03116.x

DO - 10.1002/j.2050-0416.1968.tb03116.x

M3 - Article

AN - SCOPUS:84979189595

VL - 74

SP - 200

EP - 207

JO - Journal of the Institute of Brewing

JF - Journal of the Institute of Brewing

SN - 0046-9750

IS - 2

ER -