Quantitative immunoassay of adenosine deaminase in combined immunodeficiency disease

D. A. Carson, R. Goldblum, J. E. Seegmiller

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13 Scopus citations

Abstract

The authors have developed a quantitative immunoassay for the measurement of human adenosine deaminase that requires neither purified enzyme nor monospecific antibody. With this technique, cultured fibroblasts from three immunodeficient patients were shown to contain adenosine deaminase as detected both catalytically and immunochemically. In one patient the level of immunoreactive enzyme exceeded the amount of catalytically active adenosine deaminase, suggesting a structural alteration in the enzyme molecule.

Original languageEnglish (US)
Pages (from-to)270-273
Number of pages4
JournalJournal of Immunology
Volume118
Issue number1
StatePublished - 1977
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

Cite this

Carson, D. A., Goldblum, R., & Seegmiller, J. E. (1977). Quantitative immunoassay of adenosine deaminase in combined immunodeficiency disease. Journal of Immunology, 118(1), 270-273.