Quantitative intracellular calcium sensing with two-photon fluorescence lifetime imaging microscopy

Eddy Kuwana, Gracie Vargas-Adams, Todd C. Pappas, Anton V. Liopo, Massoud Motamedi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work, we demonstrate the use of Two-Photon Fluorescence Lifetime Imaging Microscopy (TP-FLIM) for intracellular calcium ([Ca2+] i) measurement with a calcium sensitive fluorescent dye in a neuronal cell model and brain tissue. Calcium perturbations were induced via chemical stimulation and our results signify the potential of TP-FLIM for quantitative ([Ca2+]i) measurement within the physiological range of intracellular [Ca2+].

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6424
DOIs
StatePublished - 2007
Externally publishedYes
EventPhotonic Therapeutics and Diagnostics III - San Jose, CA, United States
Duration: Jan 20 2007Jan 21 2007

Other

OtherPhotonic Therapeutics and Diagnostics III
CountryUnited States
CitySan Jose, CA
Period1/20/071/21/07

Fingerprint

Calcium
Microscopic examination
Photons
Fluorescence
Imaging techniques
Brain
Dyes
Tissue

Keywords

  • Calcium sensing
  • Fluorescence lifetime imaging
  • Two-photon excitation

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kuwana, E., Vargas-Adams, G., Pappas, T. C., Liopo, A. V., & Motamedi, M. (2007). Quantitative intracellular calcium sensing with two-photon fluorescence lifetime imaging microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6424). [64242L] https://doi.org/10.1117/12.723959

Quantitative intracellular calcium sensing with two-photon fluorescence lifetime imaging microscopy. / Kuwana, Eddy; Vargas-Adams, Gracie; Pappas, Todd C.; Liopo, Anton V.; Motamedi, Massoud.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6424 2007. 64242L.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kuwana, E, Vargas-Adams, G, Pappas, TC, Liopo, AV & Motamedi, M 2007, Quantitative intracellular calcium sensing with two-photon fluorescence lifetime imaging microscopy. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6424, 64242L, Photonic Therapeutics and Diagnostics III, San Jose, CA, United States, 1/20/07. https://doi.org/10.1117/12.723959
Kuwana E, Vargas-Adams G, Pappas TC, Liopo AV, Motamedi M. Quantitative intracellular calcium sensing with two-photon fluorescence lifetime imaging microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6424. 2007. 64242L https://doi.org/10.1117/12.723959
Kuwana, Eddy ; Vargas-Adams, Gracie ; Pappas, Todd C. ; Liopo, Anton V. ; Motamedi, Massoud. / Quantitative intracellular calcium sensing with two-photon fluorescence lifetime imaging microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6424 2007.
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