Quantitative intracellular calcium sensing with two-photon fluorescence lifetime imaging microscopy

Eddy Kuwana, Gracie Vargas-Adams, Todd C. Pappas, Anton V. Liopo, Massoud Motamedi

Research output: Chapter in Book/Report/Conference proceedingConference contribution


In this work, we demonstrate the use of Two-Photon Fluorescence Lifetime Imaging Microscopy (TP-FLIM) for intracellular calcium ([Ca2+] i) measurement with a calcium sensitive fluorescent dye in a neuronal cell model and brain tissue. Calcium perturbations were induced via chemical stimulation and our results signify the potential of TP-FLIM for quantitative ([Ca2+]i) measurement within the physiological range of intracellular [Ca2+].

Original languageEnglish (US)
Title of host publicationPhotonic Therapeutics and Diagnostics III
StatePublished - 2007
Externally publishedYes
EventPhotonic Therapeutics and Diagnostics III - San Jose, CA, United States
Duration: Jan 20 2007Jan 21 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherPhotonic Therapeutics and Diagnostics III
Country/TerritoryUnited States
CitySan Jose, CA


  • Calcium sensing
  • Fluorescence lifetime imaging
  • Two-photon excitation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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