Quantitative proteomics and transcriptomics reveals metabolic differences in attracting and non-attracting human-in-mouse glioma stem cell xenografts and stromal cells

Norelle C. Wildburger; Cheryl F. Lichti; Richard D. LeDuc; Mary Schmidt; Roger A. Kroes; Joseph R. Moskal; Carol L. Nilsson

doi:10.1016/j.euprot.2015.06.006

Quantitative proteomics and transcriptomics reveals metabolic differences in attracting and non-attracting human-in-mouse glioma stem cell xenografts and stromal cells

Norelle C. Wildburger, Cheryl F. Lichti, Richard D. LeDuc, Mary Schmidt, Roger A. Kroes, Joseph R. Moskal, Carol L. Nilsson

Pharmacology & Toxicology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Bone marrow-derived human mesenchymal stem cells (BM-hMSCs) show promise as cell-based delivery vehicles for anti-glioma therapeutics, due to innate tropism for gliomas. However, in clinically relevant human-in-mouse glioma stem cell xenograft models, BM-hMSCs tropism is variable. We compared the proteomic profile of cancer and stromal cells in GSCXs that attract BM-hMSCs ("attractors") with those to do not ("non-attractors") to identify pathways that may modulate BM-hMSC homing, followed by targeted transcriptomics. The results provide the first link between fatty acid metabolism, glucose metabolism, ROS, and N-glycosylation patterns in attractors. Reciprocal expression of these pathways in the stromal cells suggests microenvironmental cross-talk.

Original language	English (US)
Pages (from-to)	94-103
Number of pages	10
Journal	EuPA Open Proteomics
Volume	8
DOIs	https://doi.org/10.1016/j.euprot.2015.06.006
State	Published - Sep 1 2015

Keywords

Bone marrow-derived human mesenchymal stem cells (BM-hMSCs)
Cancer proteomics
Fatty acid metabolism
Glioblastoma
Glycolysis
Glycosylation
Mass spectrometry
Pentose phosphate pathway
ROS
Transcriptomics

ASJC Scopus subject areas

Biochemistry

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10.1016/j.euprot.2015.06.006

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title = "Quantitative proteomics and transcriptomics reveals metabolic differences in attracting and non-attracting human-in-mouse glioma stem cell xenografts and stromal cells",

abstract = "Bone marrow-derived human mesenchymal stem cells (BM-hMSCs) show promise as cell-based delivery vehicles for anti-glioma therapeutics, due to innate tropism for gliomas. However, in clinically relevant human-in-mouse glioma stem cell xenograft models, BM-hMSCs tropism is variable. We compared the proteomic profile of cancer and stromal cells in GSCXs that attract BM-hMSCs ({"}attractors{"}) with those to do not ({"}non-attractors{"}) to identify pathways that may modulate BM-hMSC homing, followed by targeted transcriptomics. The results provide the first link between fatty acid metabolism, glucose metabolism, ROS, and N-glycosylation patterns in attractors. Reciprocal expression of these pathways in the stromal cells suggests microenvironmental cross-talk.",

keywords = "Bone marrow-derived human mesenchymal stem cells (BM-hMSCs), Cancer proteomics, Fatty acid metabolism, Glioblastoma, Glycolysis, Glycosylation, Mass spectrometry, Pentose phosphate pathway, ROS, Transcriptomics",

author = "Wildburger, {Norelle C.} and Lichti, {Cheryl F.} and LeDuc, {Richard D.} and Mary Schmidt and Kroes, {Roger A.} and Moskal, {Joseph R.} and Nilsson, {Carol L.}",

note = "Publisher Copyright: {\textcopyright} 2015 The Authors.",

year = "2015",

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doi = "10.1016/j.euprot.2015.06.006",

language = "English (US)",

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T1 - Quantitative proteomics and transcriptomics reveals metabolic differences in attracting and non-attracting human-in-mouse glioma stem cell xenografts and stromal cells

AU - Wildburger, Norelle C.

AU - Lichti, Cheryl F.

AU - LeDuc, Richard D.

AU - Schmidt, Mary

AU - Kroes, Roger A.

AU - Moskal, Joseph R.

AU - Nilsson, Carol L.

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Bone marrow-derived human mesenchymal stem cells (BM-hMSCs) show promise as cell-based delivery vehicles for anti-glioma therapeutics, due to innate tropism for gliomas. However, in clinically relevant human-in-mouse glioma stem cell xenograft models, BM-hMSCs tropism is variable. We compared the proteomic profile of cancer and stromal cells in GSCXs that attract BM-hMSCs ("attractors") with those to do not ("non-attractors") to identify pathways that may modulate BM-hMSC homing, followed by targeted transcriptomics. The results provide the first link between fatty acid metabolism, glucose metabolism, ROS, and N-glycosylation patterns in attractors. Reciprocal expression of these pathways in the stromal cells suggests microenvironmental cross-talk.

AB - Bone marrow-derived human mesenchymal stem cells (BM-hMSCs) show promise as cell-based delivery vehicles for anti-glioma therapeutics, due to innate tropism for gliomas. However, in clinically relevant human-in-mouse glioma stem cell xenograft models, BM-hMSCs tropism is variable. We compared the proteomic profile of cancer and stromal cells in GSCXs that attract BM-hMSCs ("attractors") with those to do not ("non-attractors") to identify pathways that may modulate BM-hMSC homing, followed by targeted transcriptomics. The results provide the first link between fatty acid metabolism, glucose metabolism, ROS, and N-glycosylation patterns in attractors. Reciprocal expression of these pathways in the stromal cells suggests microenvironmental cross-talk.

KW - Bone marrow-derived human mesenchymal stem cells (BM-hMSCs)

KW - Cancer proteomics

KW - Fatty acid metabolism

KW - Glioblastoma

KW - Glycolysis

KW - Glycosylation

KW - Mass spectrometry

KW - Pentose phosphate pathway

KW - ROS

KW - Transcriptomics

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DO - 10.1016/j.euprot.2015.06.006

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JO - EuPA Open Proteomics

JF - EuPA Open Proteomics

ER -

Quantitative proteomics and transcriptomics reveals metabolic differences in attracting and non-attracting human-in-mouse glioma stem cell xenografts and stromal cells

Abstract

Keywords

ASJC Scopus subject areas

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