Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs

Natacha Milhano, Vsevolod Popov, Manuela Vilhena, Donald Bouyer, Rita de Sousa, David Walker

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Rickettsia massiliae, belonging to the spotted fever group of Rickettsia, is a human pathogen causing a similar course of disease to that caused by R. conorii, the originally recognized etiologic agent of Mediterranean spotted fever. In view of this similarity, we performed an ultrastructural study of R. massiliae in organs of Rhipicephalus sanguineus ticks, in order to advance knowledge of the complex dynamics at the tick-pathogen interface in rickettsioses.Adult R. massiliae-infected Rh. sanguineus ticks were fed on uninfected Hartley strain guinea pigs, and five females were collected daily throughout their feeding period up to day 6, and analyzed by quantitative real-time PCR and electron microscopy. An increase in rickettsial content was observed in the salivary glands, particularly in the first two days of feeding, and a plateau was observed between days 3 and 6. Rickettsial organisms were observed in all tick organs analyzed, in higher numbers in the fed state, and statistically significant differences were observed in measurements of the periplasmic layer of R. massiliae in salivary glands of fed and unfed Rh. sanguineus ticks, with increased thickness in the former case.This study provides insight into the interface between R. massiliae and Rh. sanguineus ticks, highlighting the need for analysis of R. massiliae to fully ascertain its place as an important pathogenic agent of a spotted fever rickettsiosis.

Original languageEnglish (US)
Pages (from-to)709-714
Number of pages6
JournalTicks and Tick-borne Diseases
Volume5
Issue number6
DOIs
StatePublished - Oct 1 2014

Fingerprint

Rickettsia massiliae
Rhipicephalus sanguineus
Rickettsia
Ticks
ticks
salivary glands
Salivary Glands
fever
Fever
Boutonneuse Fever
rickettsial diseases
pathogens
disease course
guinea pigs
Real-Time Polymerase Chain Reaction
electron microscopy
Electron Microscopy
plateaus
Guinea Pigs
quantitative polymerase chain reaction

Keywords

  • Electron microscopy
  • Quantitative real-time PCR
  • Rhipicephalus sanguineus
  • Rickettsia massiliae
  • Ticks

ASJC Scopus subject areas

  • Infectious Diseases
  • Insect Science
  • Parasitology
  • Microbiology

Cite this

Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs. / Milhano, Natacha; Popov, Vsevolod; Vilhena, Manuela; Bouyer, Donald; de Sousa, Rita; Walker, David.

In: Ticks and Tick-borne Diseases, Vol. 5, No. 6, 01.10.2014, p. 709-714.

Research output: Contribution to journalArticle

@article{db112e09d4354668aa9c6711fa02e243,
title = "Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs",
abstract = "Rickettsia massiliae, belonging to the spotted fever group of Rickettsia, is a human pathogen causing a similar course of disease to that caused by R. conorii, the originally recognized etiologic agent of Mediterranean spotted fever. In view of this similarity, we performed an ultrastructural study of R. massiliae in organs of Rhipicephalus sanguineus ticks, in order to advance knowledge of the complex dynamics at the tick-pathogen interface in rickettsioses.Adult R. massiliae-infected Rh. sanguineus ticks were fed on uninfected Hartley strain guinea pigs, and five females were collected daily throughout their feeding period up to day 6, and analyzed by quantitative real-time PCR and electron microscopy. An increase in rickettsial content was observed in the salivary glands, particularly in the first two days of feeding, and a plateau was observed between days 3 and 6. Rickettsial organisms were observed in all tick organs analyzed, in higher numbers in the fed state, and statistically significant differences were observed in measurements of the periplasmic layer of R. massiliae in salivary glands of fed and unfed Rh. sanguineus ticks, with increased thickness in the former case.This study provides insight into the interface between R. massiliae and Rh. sanguineus ticks, highlighting the need for analysis of R. massiliae to fully ascertain its place as an important pathogenic agent of a spotted fever rickettsiosis.",
keywords = "Electron microscopy, Quantitative real-time PCR, Rhipicephalus sanguineus, Rickettsia massiliae, Ticks",
author = "Natacha Milhano and Vsevolod Popov and Manuela Vilhena and Donald Bouyer and {de Sousa}, Rita and David Walker",
year = "2014",
month = "10",
day = "1",
doi = "10.1016/j.ttbdis.2014.05.009",
language = "English (US)",
volume = "5",
pages = "709--714",
journal = "Ticks and Tick-borne Diseases",
issn = "1877-959X",
publisher = "Elsevier GmbH",
number = "6",

}

TY - JOUR

T1 - Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs

AU - Milhano, Natacha

AU - Popov, Vsevolod

AU - Vilhena, Manuela

AU - Bouyer, Donald

AU - de Sousa, Rita

AU - Walker, David

PY - 2014/10/1

Y1 - 2014/10/1

N2 - Rickettsia massiliae, belonging to the spotted fever group of Rickettsia, is a human pathogen causing a similar course of disease to that caused by R. conorii, the originally recognized etiologic agent of Mediterranean spotted fever. In view of this similarity, we performed an ultrastructural study of R. massiliae in organs of Rhipicephalus sanguineus ticks, in order to advance knowledge of the complex dynamics at the tick-pathogen interface in rickettsioses.Adult R. massiliae-infected Rh. sanguineus ticks were fed on uninfected Hartley strain guinea pigs, and five females were collected daily throughout their feeding period up to day 6, and analyzed by quantitative real-time PCR and electron microscopy. An increase in rickettsial content was observed in the salivary glands, particularly in the first two days of feeding, and a plateau was observed between days 3 and 6. Rickettsial organisms were observed in all tick organs analyzed, in higher numbers in the fed state, and statistically significant differences were observed in measurements of the periplasmic layer of R. massiliae in salivary glands of fed and unfed Rh. sanguineus ticks, with increased thickness in the former case.This study provides insight into the interface between R. massiliae and Rh. sanguineus ticks, highlighting the need for analysis of R. massiliae to fully ascertain its place as an important pathogenic agent of a spotted fever rickettsiosis.

AB - Rickettsia massiliae, belonging to the spotted fever group of Rickettsia, is a human pathogen causing a similar course of disease to that caused by R. conorii, the originally recognized etiologic agent of Mediterranean spotted fever. In view of this similarity, we performed an ultrastructural study of R. massiliae in organs of Rhipicephalus sanguineus ticks, in order to advance knowledge of the complex dynamics at the tick-pathogen interface in rickettsioses.Adult R. massiliae-infected Rh. sanguineus ticks were fed on uninfected Hartley strain guinea pigs, and five females were collected daily throughout their feeding period up to day 6, and analyzed by quantitative real-time PCR and electron microscopy. An increase in rickettsial content was observed in the salivary glands, particularly in the first two days of feeding, and a plateau was observed between days 3 and 6. Rickettsial organisms were observed in all tick organs analyzed, in higher numbers in the fed state, and statistically significant differences were observed in measurements of the periplasmic layer of R. massiliae in salivary glands of fed and unfed Rh. sanguineus ticks, with increased thickness in the former case.This study provides insight into the interface between R. massiliae and Rh. sanguineus ticks, highlighting the need for analysis of R. massiliae to fully ascertain its place as an important pathogenic agent of a spotted fever rickettsiosis.

KW - Electron microscopy

KW - Quantitative real-time PCR

KW - Rhipicephalus sanguineus

KW - Rickettsia massiliae

KW - Ticks

UR - http://www.scopus.com/inward/record.url?scp=84907694395&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907694395&partnerID=8YFLogxK

U2 - 10.1016/j.ttbdis.2014.05.009

DO - 10.1016/j.ttbdis.2014.05.009

M3 - Article

VL - 5

SP - 709

EP - 714

JO - Ticks and Tick-borne Diseases

JF - Ticks and Tick-borne Diseases

SN - 1877-959X

IS - 6

ER -