Quantity of latency-associated transcript produced by herpes simplex virus is not predictive of the frequency of experimental recurrent genital herpes

Nigel Bourne, Lawrence R. Stanberry, Beverly L. Connelly, Jumana Kurawadwala, Stephen E. Straus, Philip R. Krause

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The role of the latency-associated transcript (LAT) in control of recurrent herpes simplex virus type 2 (HSV-2) infection was investigated by examining whether LAT concentration in vitro during productive infection or in ganglia during latency correlated with the frequency of recurrent genital herpes. Clinical HSV-2 isolates from frequent or infrequent recurrent genital disease produced comparable amounts of glycoprotein D and infected cell polypeptide 0 RNA, but the isolate from frequent disease produced about seven times more LAT. The guinea pig model of genital herpes was used to determine whether the quantity of LAT produced during acute infection in vitro correlated with recurrence phenotype; the frequency of recurrent disease was similar for the 2 clinical isolates. Likewise, there was no correlation between the recurrence phenotype of individual animals and LAT concentration in their ganglia. Thus, while absence of LAT may impair HSV reactivation and recurrence, once a threshold concentration is exceeded, LAT has no further effect on recurrence frequency.

Original languageEnglish (US)
Pages (from-to)1084-1087
Number of pages4
JournalJournal of Infectious Diseases
Volume169
Issue number5
StatePublished - May 1994
Externally publishedYes

Fingerprint

Herpes Genitalis
Simplexvirus
Recurrence
Human Herpesvirus 2
Ganglia
Phenotype
Virus Diseases
Infection
Glycoproteins
Guinea Pigs
RNA
Peptides
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Quantity of latency-associated transcript produced by herpes simplex virus is not predictive of the frequency of experimental recurrent genital herpes. / Bourne, Nigel; Stanberry, Lawrence R.; Connelly, Beverly L.; Kurawadwala, Jumana; Straus, Stephen E.; Krause, Philip R.

In: Journal of Infectious Diseases, Vol. 169, No. 5, 05.1994, p. 1084-1087.

Research output: Contribution to journalArticle

Bourne, Nigel ; Stanberry, Lawrence R. ; Connelly, Beverly L. ; Kurawadwala, Jumana ; Straus, Stephen E. ; Krause, Philip R. / Quantity of latency-associated transcript produced by herpes simplex virus is not predictive of the frequency of experimental recurrent genital herpes. In: Journal of Infectious Diseases. 1994 ; Vol. 169, No. 5. pp. 1084-1087.
@article{7b82c25721304638accf94eafa8c6220,
title = "Quantity of latency-associated transcript produced by herpes simplex virus is not predictive of the frequency of experimental recurrent genital herpes",
abstract = "The role of the latency-associated transcript (LAT) in control of recurrent herpes simplex virus type 2 (HSV-2) infection was investigated by examining whether LAT concentration in vitro during productive infection or in ganglia during latency correlated with the frequency of recurrent genital herpes. Clinical HSV-2 isolates from frequent or infrequent recurrent genital disease produced comparable amounts of glycoprotein D and infected cell polypeptide 0 RNA, but the isolate from frequent disease produced about seven times more LAT. The guinea pig model of genital herpes was used to determine whether the quantity of LAT produced during acute infection in vitro correlated with recurrence phenotype; the frequency of recurrent disease was similar for the 2 clinical isolates. Likewise, there was no correlation between the recurrence phenotype of individual animals and LAT concentration in their ganglia. Thus, while absence of LAT may impair HSV reactivation and recurrence, once a threshold concentration is exceeded, LAT has no further effect on recurrence frequency.",
author = "Nigel Bourne and Stanberry, {Lawrence R.} and Connelly, {Beverly L.} and Jumana Kurawadwala and Straus, {Stephen E.} and Krause, {Philip R.}",
year = "1994",
month = "5",
language = "English (US)",
volume = "169",
pages = "1084--1087",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - Quantity of latency-associated transcript produced by herpes simplex virus is not predictive of the frequency of experimental recurrent genital herpes

AU - Bourne, Nigel

AU - Stanberry, Lawrence R.

AU - Connelly, Beverly L.

AU - Kurawadwala, Jumana

AU - Straus, Stephen E.

AU - Krause, Philip R.

PY - 1994/5

Y1 - 1994/5

N2 - The role of the latency-associated transcript (LAT) in control of recurrent herpes simplex virus type 2 (HSV-2) infection was investigated by examining whether LAT concentration in vitro during productive infection or in ganglia during latency correlated with the frequency of recurrent genital herpes. Clinical HSV-2 isolates from frequent or infrequent recurrent genital disease produced comparable amounts of glycoprotein D and infected cell polypeptide 0 RNA, but the isolate from frequent disease produced about seven times more LAT. The guinea pig model of genital herpes was used to determine whether the quantity of LAT produced during acute infection in vitro correlated with recurrence phenotype; the frequency of recurrent disease was similar for the 2 clinical isolates. Likewise, there was no correlation between the recurrence phenotype of individual animals and LAT concentration in their ganglia. Thus, while absence of LAT may impair HSV reactivation and recurrence, once a threshold concentration is exceeded, LAT has no further effect on recurrence frequency.

AB - The role of the latency-associated transcript (LAT) in control of recurrent herpes simplex virus type 2 (HSV-2) infection was investigated by examining whether LAT concentration in vitro during productive infection or in ganglia during latency correlated with the frequency of recurrent genital herpes. Clinical HSV-2 isolates from frequent or infrequent recurrent genital disease produced comparable amounts of glycoprotein D and infected cell polypeptide 0 RNA, but the isolate from frequent disease produced about seven times more LAT. The guinea pig model of genital herpes was used to determine whether the quantity of LAT produced during acute infection in vitro correlated with recurrence phenotype; the frequency of recurrent disease was similar for the 2 clinical isolates. Likewise, there was no correlation between the recurrence phenotype of individual animals and LAT concentration in their ganglia. Thus, while absence of LAT may impair HSV reactivation and recurrence, once a threshold concentration is exceeded, LAT has no further effect on recurrence frequency.

UR - http://www.scopus.com/inward/record.url?scp=0027934653&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027934653&partnerID=8YFLogxK

M3 - Article

VL - 169

SP - 1084

EP - 1087

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 5

ER -