Quaranfil, Johnston Atoll, and Lake Chad viruses are novel members of the family Orthomyxoviridae

Rachel M. Presti, Guoyan Zhao, Wandy L. Beatty, Kathie A. Mihindukulasuriya, Amelia P A Travassos Da Rosa, Vsevolod Popov, Robert B. Tesh, Herbert W. Virgin, David Wang

Research output: Contribution to journalArticle

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Abstract

Arboviral infections are an important cause of emerging infections due to the movements of humans, animals, and hematophagous arthropods. Quaranfil virus (QRFV) is an unclassified arbovirus originally isolated from children with mild febrile illness in Quaranfil, Egypt, in 1953. It has subsequently been isolated in multiple geographic areas from ticks and birds. We used high-throughput sequencing to classify QRFV as a novel orthomyxovirus. The genome of this virus is comprised of multiple RNA segments; five were completely sequenced. Proteins with limited amino acid similarity to conserved domains in polymerase (PA, PB1, and PB2) and hemagglutinin (HA) genes from known orthomyxoviruses were predicted to be present in four of the segments. The fifth sequenced segment shared no detectable similarity to any protein and is of uncertain function. The end-terminal sequences of QRFV are conserved between segments and are different from those of the known orthomyxovirus genera. QRFV is known to cross-react serologically with two other unclassified viruses, Johnston Atoll virus (JAV) and Lake Chad virus (LKCV). The complete open reading frames of PB1 and HA were sequenced for JAV, while a fragment of PB1 of LKCV was identified by mass sequencing. QRFV and JAV PB1 and HA shared 80% and 70% amino acid identity to each other, respectively; the LKCV PB1 fragment shared 83% amino acid identity with the corresponding region of QRFV PB1. Based on phylogenetic analyses, virion ultrastructural features, and the unique end-terminal sequences identified, we propose that QRFV, JAV, and LKCV comprise a novel genus of the family Orthomyxoviridae.

Original languageEnglish (US)
Pages (from-to)11599-11606
Number of pages8
JournalJournal of Virology
Volume83
Issue number22
DOIs
StatePublished - Nov 2009

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Quaranfil virus
Lake Chad
Chad
Orthomyxoviridae
Lakes
Viruses
viruses
hemagglutinins
amino acids
Unclassified Viruses
unassigned viruses
Hemagglutinins
hematophagous arthropods
arboviruses
emerging diseases
virion
Egypt
Amino Acids
fever
open reading frames

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Presti, R. M., Zhao, G., Beatty, W. L., Mihindukulasuriya, K. A., Travassos Da Rosa, A. P. A., Popov, V., ... Wang, D. (2009). Quaranfil, Johnston Atoll, and Lake Chad viruses are novel members of the family Orthomyxoviridae. Journal of Virology, 83(22), 11599-11606. https://doi.org/10.1128/JVI.00677-09

Quaranfil, Johnston Atoll, and Lake Chad viruses are novel members of the family Orthomyxoviridae. / Presti, Rachel M.; Zhao, Guoyan; Beatty, Wandy L.; Mihindukulasuriya, Kathie A.; Travassos Da Rosa, Amelia P A; Popov, Vsevolod; Tesh, Robert B.; Virgin, Herbert W.; Wang, David.

In: Journal of Virology, Vol. 83, No. 22, 11.2009, p. 11599-11606.

Research output: Contribution to journalArticle

Presti, RM, Zhao, G, Beatty, WL, Mihindukulasuriya, KA, Travassos Da Rosa, APA, Popov, V, Tesh, RB, Virgin, HW & Wang, D 2009, 'Quaranfil, Johnston Atoll, and Lake Chad viruses are novel members of the family Orthomyxoviridae', Journal of Virology, vol. 83, no. 22, pp. 11599-11606. https://doi.org/10.1128/JVI.00677-09
Presti RM, Zhao G, Beatty WL, Mihindukulasuriya KA, Travassos Da Rosa APA, Popov V et al. Quaranfil, Johnston Atoll, and Lake Chad viruses are novel members of the family Orthomyxoviridae. Journal of Virology. 2009 Nov;83(22):11599-11606. https://doi.org/10.1128/JVI.00677-09
Presti, Rachel M. ; Zhao, Guoyan ; Beatty, Wandy L. ; Mihindukulasuriya, Kathie A. ; Travassos Da Rosa, Amelia P A ; Popov, Vsevolod ; Tesh, Robert B. ; Virgin, Herbert W. ; Wang, David. / Quaranfil, Johnston Atoll, and Lake Chad viruses are novel members of the family Orthomyxoviridae. In: Journal of Virology. 2009 ; Vol. 83, No. 22. pp. 11599-11606.
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