rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain

Lisa Elferink, Kaijiro Anzai, Richard H. Scheller

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

rab3A is a low molecular weight (LMW) GTP-binding protein specifically expressed in brain and localized to synaptic vesicles. rab3A has been proposed to play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. In an attempt to define other LMW GTP-binding proteins that may regulate neurotransmitter release, seven cDNA clones encoding new members of the rab family of LMW GTP-binding proteins were isolated from a rat brain cDNA library. The rab proteins contain the four conserved structural domains essential for GTP binding in addition to domains required for membrane localization and effector protein interactions. One protein, rab16, is closely related to members of the rab3 subfamily, whereas two others are assigned as the rat homologs of canine rab8 and rab10. Four additional clones, rab12, rab13, rab14, and rab15, revealed unique sequences and are new members of the rab family of LMW GTP-binding proteins. The patterns of expression of rab15 and rab3A closely overlap but differ from that observed for all other known LMW GTP-binding proteins. This data suggests that rab15 may act in concert with rab3A in regulating aspects of synaptic vesicle membrane flow within the nerve terminal.

Original languageEnglish (US)
Pages (from-to)5768-5775
Number of pages8
JournalJournal of Biological Chemistry
Volume267
Issue number9
StatePublished - Mar 25 1992
Externally publishedYes

Fingerprint

GTP-Binding Proteins
Rats
Brain
Molecular Weight
Molecular weight
Synaptic Vesicles
Membranes
Neurotransmitter Agents
Clone Cells
Synaptic Membranes
Proteins
Guanosine Triphosphate
Gene Library
Canidae
Complementary DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain. / Elferink, Lisa; Anzai, Kaijiro; Scheller, Richard H.

In: Journal of Biological Chemistry, Vol. 267, No. 9, 25.03.1992, p. 5768-5775.

Research output: Contribution to journalArticle

@article{af694fb7bf7b416b83b48f1822d70645,
title = "rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain",
abstract = "rab3A is a low molecular weight (LMW) GTP-binding protein specifically expressed in brain and localized to synaptic vesicles. rab3A has been proposed to play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. In an attempt to define other LMW GTP-binding proteins that may regulate neurotransmitter release, seven cDNA clones encoding new members of the rab family of LMW GTP-binding proteins were isolated from a rat brain cDNA library. The rab proteins contain the four conserved structural domains essential for GTP binding in addition to domains required for membrane localization and effector protein interactions. One protein, rab16, is closely related to members of the rab3 subfamily, whereas two others are assigned as the rat homologs of canine rab8 and rab10. Four additional clones, rab12, rab13, rab14, and rab15, revealed unique sequences and are new members of the rab family of LMW GTP-binding proteins. The patterns of expression of rab15 and rab3A closely overlap but differ from that observed for all other known LMW GTP-binding proteins. This data suggests that rab15 may act in concert with rab3A in regulating aspects of synaptic vesicle membrane flow within the nerve terminal.",
author = "Lisa Elferink and Kaijiro Anzai and Scheller, {Richard H.}",
year = "1992",
month = "3",
day = "25",
language = "English (US)",
volume = "267",
pages = "5768--5775",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "9",

}

TY - JOUR

T1 - rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain

AU - Elferink, Lisa

AU - Anzai, Kaijiro

AU - Scheller, Richard H.

PY - 1992/3/25

Y1 - 1992/3/25

N2 - rab3A is a low molecular weight (LMW) GTP-binding protein specifically expressed in brain and localized to synaptic vesicles. rab3A has been proposed to play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. In an attempt to define other LMW GTP-binding proteins that may regulate neurotransmitter release, seven cDNA clones encoding new members of the rab family of LMW GTP-binding proteins were isolated from a rat brain cDNA library. The rab proteins contain the four conserved structural domains essential for GTP binding in addition to domains required for membrane localization and effector protein interactions. One protein, rab16, is closely related to members of the rab3 subfamily, whereas two others are assigned as the rat homologs of canine rab8 and rab10. Four additional clones, rab12, rab13, rab14, and rab15, revealed unique sequences and are new members of the rab family of LMW GTP-binding proteins. The patterns of expression of rab15 and rab3A closely overlap but differ from that observed for all other known LMW GTP-binding proteins. This data suggests that rab15 may act in concert with rab3A in regulating aspects of synaptic vesicle membrane flow within the nerve terminal.

AB - rab3A is a low molecular weight (LMW) GTP-binding protein specifically expressed in brain and localized to synaptic vesicles. rab3A has been proposed to play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. In an attempt to define other LMW GTP-binding proteins that may regulate neurotransmitter release, seven cDNA clones encoding new members of the rab family of LMW GTP-binding proteins were isolated from a rat brain cDNA library. The rab proteins contain the four conserved structural domains essential for GTP binding in addition to domains required for membrane localization and effector protein interactions. One protein, rab16, is closely related to members of the rab3 subfamily, whereas two others are assigned as the rat homologs of canine rab8 and rab10. Four additional clones, rab12, rab13, rab14, and rab15, revealed unique sequences and are new members of the rab family of LMW GTP-binding proteins. The patterns of expression of rab15 and rab3A closely overlap but differ from that observed for all other known LMW GTP-binding proteins. This data suggests that rab15 may act in concert with rab3A in regulating aspects of synaptic vesicle membrane flow within the nerve terminal.

UR - http://www.scopus.com/inward/record.url?scp=0026700247&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026700247&partnerID=8YFLogxK

M3 - Article

C2 - 1313420

AN - SCOPUS:0026700247

VL - 267

SP - 5768

EP - 5775

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 9

ER -