Rab15 effector protein

A novel protein for receptor recycling from the endocytic recycling compartment

David J. Strick, Lisa Elferink

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Sorting endosomes and the endocytic recycling compartment are critical intracellular stores for the rapid recycling of internalized membrane receptors to the cell surface in multiple cell types. However, the molecular mechanisms distinguishing fast receptor recycling from sorting endosomes and slow receptor recycling from the endocytic recycling compartment remain poorly understood. We previously reported that Rab15 differentially regulates transferrin receptor trafficking through sorting endosomes and the endocytic recycling compartment, suggesting a role for distinct Rab15-effector interactions at these endocytic compartments. In this study, we identified the novel protein Rab15 effector protein (REP15) as a binding partner for Rab15-GTP. REP15 is compartment specific, colocalizing with Rab15 and Rab11 on the endocytic recycling compartment but not with Rab15, Rab4, or early endosome antigen 1 on sorting endosomes. REP15 interacts directly with Rab15-GTP but not with Rab5 or Rab11. Consistent with its localization, REP15 overexpression and small interfering RNA-mediated depletion inhibited transferrin receptor recycling from the endocytic recycling compartment, without affecting receptor entry into or recycling from sorting endosomes. Our data identify REP15 as a compartment-specific protein for receptor recycling from the endocytic recycling compartment, highlighting that the rapid and slow modes of transferrin receptor recycling are mechanistically distinct pathways.

Original languageEnglish (US)
Pages (from-to)5699-5709
Number of pages11
JournalMolecular Biology of the Cell
Volume16
Issue number12
DOIs
StatePublished - Dec 2005

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Endosomes
Staphylococcal Protein A
Transferrin Receptors
Recycling
Guanosine Triphosphate
Proteins
Cell Surface Receptors
Small Interfering RNA
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Rab15 effector protein : A novel protein for receptor recycling from the endocytic recycling compartment. / Strick, David J.; Elferink, Lisa.

In: Molecular Biology of the Cell, Vol. 16, No. 12, 12.2005, p. 5699-5709.

Research output: Contribution to journalArticle

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