Rabbit hindlimb glucose uptake assessed with positron-emitting fluorodeoxyglucose

K. A. Mossberg, R. W. Rowe, T. J. Tewson, H. Taegtmeyer

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The feasibility of estimating skeletal muscle glucose uptake in vivo was examined by using the glucose analogue 2-[18F]deoxy-2-fluoro-D-glucose (2-[18F]FDG) in the rabbit hindlimb. A pair of collimated coincidence gamma photon detectors was used to monitor the accumulation of tracer in the tissue after 2-[18F]FDG injection. Time-activity curves were generated on a second-by-second basis under control conditions, during increased contractile activity, or hyperinsulinemia. The arterial input of 2-[18F]FDG, plasma glucose, lactate, free fatty acids, and insulin were determined. A graphical (Patlak plot) procedure was used to determine the fractional rate of tracer phosphorylation and therefore trapping in the muscle. From the graphical analysis, the estimated rate of glucose phosphorylation (R) in the unperturbed state was calculated to be 0.037 μmol · min-1 · ml-1 of tissue. During perturbation by electrical stimulation, an increase in the rate of tracer phosphorylation (K) was observed. No change in the rate of tracer phosphorylation was observed during hyperinsulinemia. The results support the use of 2-[18F]FDG and the graphical procedure for the noninvasive assessment of glucose uptake by skeletal muscle in vivo. The method described is sensitive to changes in the rate of tracer uptake with respect to time and physiological interventions.

Original languageEnglish (US)
Pages (from-to)1569-1577
Number of pages9
JournalJournal of Applied Physiology
Volume67
Issue number4
StatePublished - 1989
Externally publishedYes

Fingerprint

Hindlimb
Fluorodeoxyglucose F18
Electrons
Rabbits
Glucose
Phosphorylation
Hyperinsulinism
Skeletal Muscle
Photons
Nonesterified Fatty Acids
Electric Stimulation
Lactic Acid
Insulin
Muscles
Injections

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Mossberg, K. A., Rowe, R. W., Tewson, T. J., & Taegtmeyer, H. (1989). Rabbit hindlimb glucose uptake assessed with positron-emitting fluorodeoxyglucose. Journal of Applied Physiology, 67(4), 1569-1577.

Rabbit hindlimb glucose uptake assessed with positron-emitting fluorodeoxyglucose. / Mossberg, K. A.; Rowe, R. W.; Tewson, T. J.; Taegtmeyer, H.

In: Journal of Applied Physiology, Vol. 67, No. 4, 1989, p. 1569-1577.

Research output: Contribution to journalArticle

Mossberg, KA, Rowe, RW, Tewson, TJ & Taegtmeyer, H 1989, 'Rabbit hindlimb glucose uptake assessed with positron-emitting fluorodeoxyglucose', Journal of Applied Physiology, vol. 67, no. 4, pp. 1569-1577.
Mossberg, K. A. ; Rowe, R. W. ; Tewson, T. J. ; Taegtmeyer, H. / Rabbit hindlimb glucose uptake assessed with positron-emitting fluorodeoxyglucose. In: Journal of Applied Physiology. 1989 ; Vol. 67, No. 4. pp. 1569-1577.
@article{6a99a7743b8d4874921852f29b779119,
title = "Rabbit hindlimb glucose uptake assessed with positron-emitting fluorodeoxyglucose",
abstract = "The feasibility of estimating skeletal muscle glucose uptake in vivo was examined by using the glucose analogue 2-[18F]deoxy-2-fluoro-D-glucose (2-[18F]FDG) in the rabbit hindlimb. A pair of collimated coincidence gamma photon detectors was used to monitor the accumulation of tracer in the tissue after 2-[18F]FDG injection. Time-activity curves were generated on a second-by-second basis under control conditions, during increased contractile activity, or hyperinsulinemia. The arterial input of 2-[18F]FDG, plasma glucose, lactate, free fatty acids, and insulin were determined. A graphical (Patlak plot) procedure was used to determine the fractional rate of tracer phosphorylation and therefore trapping in the muscle. From the graphical analysis, the estimated rate of glucose phosphorylation (R) in the unperturbed state was calculated to be 0.037 μmol · min-1 · ml-1 of tissue. During perturbation by electrical stimulation, an increase in the rate of tracer phosphorylation (K) was observed. No change in the rate of tracer phosphorylation was observed during hyperinsulinemia. The results support the use of 2-[18F]FDG and the graphical procedure for the noninvasive assessment of glucose uptake by skeletal muscle in vivo. The method described is sensitive to changes in the rate of tracer uptake with respect to time and physiological interventions.",
author = "Mossberg, {K. A.} and Rowe, {R. W.} and Tewson, {T. J.} and H. Taegtmeyer",
year = "1989",
language = "English (US)",
volume = "67",
pages = "1569--1577",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "4",

}

TY - JOUR

T1 - Rabbit hindlimb glucose uptake assessed with positron-emitting fluorodeoxyglucose

AU - Mossberg, K. A.

AU - Rowe, R. W.

AU - Tewson, T. J.

AU - Taegtmeyer, H.

PY - 1989

Y1 - 1989

N2 - The feasibility of estimating skeletal muscle glucose uptake in vivo was examined by using the glucose analogue 2-[18F]deoxy-2-fluoro-D-glucose (2-[18F]FDG) in the rabbit hindlimb. A pair of collimated coincidence gamma photon detectors was used to monitor the accumulation of tracer in the tissue after 2-[18F]FDG injection. Time-activity curves were generated on a second-by-second basis under control conditions, during increased contractile activity, or hyperinsulinemia. The arterial input of 2-[18F]FDG, plasma glucose, lactate, free fatty acids, and insulin were determined. A graphical (Patlak plot) procedure was used to determine the fractional rate of tracer phosphorylation and therefore trapping in the muscle. From the graphical analysis, the estimated rate of glucose phosphorylation (R) in the unperturbed state was calculated to be 0.037 μmol · min-1 · ml-1 of tissue. During perturbation by electrical stimulation, an increase in the rate of tracer phosphorylation (K) was observed. No change in the rate of tracer phosphorylation was observed during hyperinsulinemia. The results support the use of 2-[18F]FDG and the graphical procedure for the noninvasive assessment of glucose uptake by skeletal muscle in vivo. The method described is sensitive to changes in the rate of tracer uptake with respect to time and physiological interventions.

AB - The feasibility of estimating skeletal muscle glucose uptake in vivo was examined by using the glucose analogue 2-[18F]deoxy-2-fluoro-D-glucose (2-[18F]FDG) in the rabbit hindlimb. A pair of collimated coincidence gamma photon detectors was used to monitor the accumulation of tracer in the tissue after 2-[18F]FDG injection. Time-activity curves were generated on a second-by-second basis under control conditions, during increased contractile activity, or hyperinsulinemia. The arterial input of 2-[18F]FDG, plasma glucose, lactate, free fatty acids, and insulin were determined. A graphical (Patlak plot) procedure was used to determine the fractional rate of tracer phosphorylation and therefore trapping in the muscle. From the graphical analysis, the estimated rate of glucose phosphorylation (R) in the unperturbed state was calculated to be 0.037 μmol · min-1 · ml-1 of tissue. During perturbation by electrical stimulation, an increase in the rate of tracer phosphorylation (K) was observed. No change in the rate of tracer phosphorylation was observed during hyperinsulinemia. The results support the use of 2-[18F]FDG and the graphical procedure for the noninvasive assessment of glucose uptake by skeletal muscle in vivo. The method described is sensitive to changes in the rate of tracer uptake with respect to time and physiological interventions.

UR - http://www.scopus.com/inward/record.url?scp=0024450456&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024450456&partnerID=8YFLogxK

M3 - Article

VL - 67

SP - 1569

EP - 1577

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 4

ER -