RACK-1, a receptor for activated C kinase, interacts with the transcription factor NFAT and represses its transactivation

Juhyun Han, Minjung Kim, Ji Hyun Oum, Heejoon Myung, Seong Wook Lee, Sunjoo Jeong, Surendra Sharma, Jungchan Park

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

To isolate and characterize a novel protein that interacts with nuclear factor of activated T cells (NFAT) and potentially regulates its activity, we screened a Jurkat cDNA library by using the NFAT regulatory domain as bait in the yeast two-hybrid system. RACK-1, a receptor for activated protein kinase C and a homologue of the G-protein β subunit, was identified as a NFAT-binding protein. Mammalian two hybrid tests in CV-1 cells and a coimmunoprecipitation assay confirmed protein-protein interaction between NFAT and RACK-1. In addition, overexpression of RACK-1 specifically suppressed transcriptional activation derived by NFAT, but not by NF-κB. These results demonstrate RACK-1 as a potent negative modulator of NFAT activation and suggest a novel mechanism in NFAT regulation.

Original languageEnglish (US)
Pages (from-to)420-424
Number of pages5
JournalMolecules and Cells
Volume14
Issue number3
StatePublished - Dec 2002
Externally publishedYes

Keywords

  • NFAT
  • RACK-1
  • Transcription Factor
  • Yeast-Two Hybrid

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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