RAD26, the yeast homolog of human Cockayne's syndrome group B gene, encodes a DNA-dependent ATPase

Sami N. Guzder, Yvette Habraken, Patrick Sung, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Cells from Cockayne's syndrome (CS) patients are sensitive to ultraviolet light and defective in preferential repair of the transcribed DNA strand. CS patients suffer from complex clinical symptoms, including severe growth retardation, neurological degeneration, mental retardation, and cachexia. Two CS complementation groups, CSA and CSB, have been identified so far. RAD26 encodes the yeast counterpart of the CSB gene. Here, we purify Rad26 protein to near homogeneity from yeast cells and show that it is a DNA- dependent ATPase. In contrast to the Mfd protein that functions in transcription.coupled repair in Escherichia coli, and which is a weak and DNA independent ATPase, Rad26 is a much more active ATPase, with a strict dependence on DNA. The possible role of Rad26 ATPase in the displacement of stalled RNA polymerase II from the site of the DNA lesion and in the subsequent recruitment of a DNA repair component is discussed.

Original languageEnglish (US)
Pages (from-to)18314-18317
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number31
DOIs
StatePublished - Oct 7 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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