RAD3 protein of Saccharomyces cerevisiae is a DNA helicase.

P. Sung, Louise Prakash, S. W. Matson, Satya Prakash

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

The Saccharomyces cerevisiae RAD3 gene, which is required for cell viability and excision repair of damaged DNA, encodes an 89-kDa protein that has a single-stranded DNA-dependent ATPase activity. We now show that the RAD3 protein also possesses a helicase activity that unwinds duplex regions in DNA substrates constructed by annealing DNA fragments of 71-851 nucleotides to circular, single-stranded M13 DNA. The DNA helicase activity is dependent on the hydrolysis of ATP, has a pH optimum of approximately 5.6, and is inhibited by antibodies raised against a truncated RAD3 protein produced in Escherichia coli. The RAD3 helicase translocates along single-stranded DNA in the 5'----3' direction. The direction of RAD3 helicase movement is consistent with the possibility that it unwinds DNA duplexes in advance of the replication fork during DNA replication.

Original languageEnglish (US)
Pages (from-to)8951-8955
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number24
StatePublished - Dec 1987
Externally publishedYes

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DNA Helicases
Saccharomyces cerevisiae Proteins
Single-Stranded DNA
DNA
Proteins
DNA Replication
DNA Repair
Saccharomyces cerevisiae
Cell Survival
Hydrolysis
Nucleotides
Adenosine Triphosphate
Escherichia coli
Antibodies
Genes
Direction compound

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

RAD3 protein of Saccharomyces cerevisiae is a DNA helicase. / Sung, P.; Prakash, Louise; Matson, S. W.; Prakash, Satya.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 24, 12.1987, p. 8951-8955.

Research output: Contribution to journalArticle

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