Radiation leukemogenesis in mice

Loss of pu.1 on chromosome 2 in CBA and C57BL/6 mice after irradiation with 1 GeV/nucleon 56Fe Ions, X Rays or γ Rays. Part I. Experimental observations

Yuanlin Peng, Natalie Brown, Rosemary Finnon, Christy L. Warner, Xianan Liu, Paula C. Genik, Matthew A. Callan, F. Andrew Ray, Thomas B. Borak, Christophe Badie, Simon D. Bouffler, Robert L. Ullrich, Joel S. Bedford, Michael M. Weil

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Since deletion of the PU.1 gene on chromosome 2 is a crucial acute myeloid leukemia (AML) initiating step in the mouse model, we quantified PU.1 deleted cells in the bone marrow of γ-, X- and 56Fe-ion-irradiated mice at various times postirradiation. Although 56Fe ions were initially some two to three times more effective than X or γ rays in inducing PU.1 deletions, by 1 month postirradiation, the proportions of cells with PU.1 deletions were similar for the HZE particles and the sparsely ionizing radiations. These results indicate that while 56Fe ions are more effective in inducing PU.1 deletions, they are also more effective in causing collateral damage that removes hit cells from the bone marrow. After X, γ or 56Fe-ion irradiation, AML-resistant C57BL/6 mice have fewer cells with PU.1 deletions than CBA mice, and those cells do not persist in the bone marrow of the C57B6/6 mice. Our findings suggest that quantification of PU.1 deleted bone marrow cells 1 month postirradiation can be used as surrogate for the incidence of radiation-induced AML measured in large-scale mouse studies. If so, PU.1 loss could be used to systematically assess the potential leukemogenic effects of other ions and energies in the space radiation environment.

Original languageEnglish (US)
Pages (from-to)474-483
Number of pages10
JournalRadiation Research
Volume171
Issue number4
DOIs
StatePublished - Apr 2009
Externally publishedYes

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Chromosomes, Human, Pair 2
chromosomes
deletion
Inbred C57BL Mouse
mice
rays
bone marrow
X-Rays
Ions
Radiation
irradiation
leukemias
Acute Myeloid Leukemia
Bone Marrow Cells
radiation
cells
ions
x rays
Cosmic Radiation
Inbred CBA Mouse

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Radiation leukemogenesis in mice : Loss of pu.1 on chromosome 2 in CBA and C57BL/6 mice after irradiation with 1 GeV/nucleon 56Fe Ions, X Rays or γ Rays. Part I. Experimental observations. / Peng, Yuanlin; Brown, Natalie; Finnon, Rosemary; Warner, Christy L.; Liu, Xianan; Genik, Paula C.; Callan, Matthew A.; Ray, F. Andrew; Borak, Thomas B.; Badie, Christophe; Bouffler, Simon D.; Ullrich, Robert L.; Bedford, Joel S.; Weil, Michael M.

In: Radiation Research, Vol. 171, No. 4, 04.2009, p. 474-483.

Research output: Contribution to journalArticle

Peng, Y, Brown, N, Finnon, R, Warner, CL, Liu, X, Genik, PC, Callan, MA, Ray, FA, Borak, TB, Badie, C, Bouffler, SD, Ullrich, RL, Bedford, JS & Weil, MM 2009, 'Radiation leukemogenesis in mice: Loss of pu.1 on chromosome 2 in CBA and C57BL/6 mice after irradiation with 1 GeV/nucleon 56Fe Ions, X Rays or γ Rays. Part I. Experimental observations', Radiation Research, vol. 171, no. 4, pp. 474-483. https://doi.org/10.1667/RR1547.1
Peng, Yuanlin ; Brown, Natalie ; Finnon, Rosemary ; Warner, Christy L. ; Liu, Xianan ; Genik, Paula C. ; Callan, Matthew A. ; Ray, F. Andrew ; Borak, Thomas B. ; Badie, Christophe ; Bouffler, Simon D. ; Ullrich, Robert L. ; Bedford, Joel S. ; Weil, Michael M. / Radiation leukemogenesis in mice : Loss of pu.1 on chromosome 2 in CBA and C57BL/6 mice after irradiation with 1 GeV/nucleon 56Fe Ions, X Rays or γ Rays. Part I. Experimental observations. In: Radiation Research. 2009 ; Vol. 171, No. 4. pp. 474-483.
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