TY - JOUR
T1 - Radiation quality and intra-chromosomal aberrations
T2 - Size matters
AU - Cornforth, Michael N.
AU - Durante, Marco
N1 - Funding Information:
The authors are indebted to Dr. Bradford Loucas for valuable discussion, and Dr. Yongjia Yu for help with aCGH analysis. This work was supported by the following grants from the National Aeronautics and Space Administration (NASA): NNX15AG74G and NNX14AC76G .
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/12
Y1 - 2018/12
N2 - The shift from plant to mammalian cell models in radiation cytogenetics hastened the development of methods suitable for the analysis of chromosome-type aberrations. These included methods to detect interchanges that take place between different chromosomes (dicentrics and translocations), and intrachanges occurring within a given chromosome (rings, interstitial deletions and inversions). In this review we consider the relationship between chromosome-type interchanges and intrachanges in response to changes in ionization density (linear energy transfer; LET). In that context, we discuss advantages and disadvantages of more modern methods used to measure intrachanges, and the implications that their increased resolution of measurement may have on the inter-to-intrachange fraction (i.e., the F-ratio). We conclude that the premise of the F-ratio is supported by its biophysical assumptions, but its intended use as an LET-dependent measure of prior radiation exposure is hampered mainly by our inability to accurately assess, on a cell-by-cell basis, inversions and interstitial deletions whose small sizes are below the detection limits of conventional cytogenetic techniques.
AB - The shift from plant to mammalian cell models in radiation cytogenetics hastened the development of methods suitable for the analysis of chromosome-type aberrations. These included methods to detect interchanges that take place between different chromosomes (dicentrics and translocations), and intrachanges occurring within a given chromosome (rings, interstitial deletions and inversions). In this review we consider the relationship between chromosome-type interchanges and intrachanges in response to changes in ionization density (linear energy transfer; LET). In that context, we discuss advantages and disadvantages of more modern methods used to measure intrachanges, and the implications that their increased resolution of measurement may have on the inter-to-intrachange fraction (i.e., the F-ratio). We conclude that the premise of the F-ratio is supported by its biophysical assumptions, but its intended use as an LET-dependent measure of prior radiation exposure is hampered mainly by our inability to accurately assess, on a cell-by-cell basis, inversions and interstitial deletions whose small sizes are below the detection limits of conventional cytogenetic techniques.
KW - Chromosome aberrations
KW - F-ratio
KW - Inversions
KW - Ionizing radiation
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U2 - 10.1016/j.mrgentox.2018.05.002
DO - 10.1016/j.mrgentox.2018.05.002
M3 - Short survey
C2 - 30389158
AN - SCOPUS:85046678080
VL - 836
SP - 28
EP - 35
JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
SN - 1383-5718
ER -