Abstract
The shift from plant to mammalian cell models in radiation cytogenetics hastened the development of methods suitable for the analysis of chromosome-type aberrations. These included methods to detect interchanges that take place between different chromosomes (dicentrics and translocations), and intrachanges occurring within a given chromosome (rings, interstitial deletions and inversions). In this review we consider the relationship between chromosome-type interchanges and intrachanges in response to changes in ionization density (linear energy transfer; LET). In that context, we discuss advantages and disadvantages of more modern methods used to measure intrachanges, and the implications that their increased resolution of measurement may have on the inter-to-intrachange fraction (i.e., the F-ratio). We conclude that the premise of the F-ratio is supported by its biophysical assumptions, but its intended use as an LET-dependent measure of prior radiation exposure is hampered mainly by our inability to accurately assess, on a cell-by-cell basis, inversions and interstitial deletions whose small sizes are below the detection limits of conventional cytogenetic techniques.
| Original language | English (US) |
|---|---|
| Journal | Mutation Research - Genetic Toxicology and Environmental Mutagenesis |
| DOIs | |
| State | Accepted/In press - Jan 1 2018 |
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Keywords
- Chromosome aberrations
- F-ratio
- Inversions
- Ionizing radiation
ASJC Scopus subject areas
- Genetics
- Health, Toxicology and Mutagenesis
Cite this
Radiation quality and intra-chromosomal aberrations : Size matters. / Cornforth, Michael; Durante, Marco.
In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 01.01.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Radiation quality and intra-chromosomal aberrations
T2 - Size matters
AU - Cornforth, Michael
AU - Durante, Marco
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The shift from plant to mammalian cell models in radiation cytogenetics hastened the development of methods suitable for the analysis of chromosome-type aberrations. These included methods to detect interchanges that take place between different chromosomes (dicentrics and translocations), and intrachanges occurring within a given chromosome (rings, interstitial deletions and inversions). In this review we consider the relationship between chromosome-type interchanges and intrachanges in response to changes in ionization density (linear energy transfer; LET). In that context, we discuss advantages and disadvantages of more modern methods used to measure intrachanges, and the implications that their increased resolution of measurement may have on the inter-to-intrachange fraction (i.e., the F-ratio). We conclude that the premise of the F-ratio is supported by its biophysical assumptions, but its intended use as an LET-dependent measure of prior radiation exposure is hampered mainly by our inability to accurately assess, on a cell-by-cell basis, inversions and interstitial deletions whose small sizes are below the detection limits of conventional cytogenetic techniques.
AB - The shift from plant to mammalian cell models in radiation cytogenetics hastened the development of methods suitable for the analysis of chromosome-type aberrations. These included methods to detect interchanges that take place between different chromosomes (dicentrics and translocations), and intrachanges occurring within a given chromosome (rings, interstitial deletions and inversions). In this review we consider the relationship between chromosome-type interchanges and intrachanges in response to changes in ionization density (linear energy transfer; LET). In that context, we discuss advantages and disadvantages of more modern methods used to measure intrachanges, and the implications that their increased resolution of measurement may have on the inter-to-intrachange fraction (i.e., the F-ratio). We conclude that the premise of the F-ratio is supported by its biophysical assumptions, but its intended use as an LET-dependent measure of prior radiation exposure is hampered mainly by our inability to accurately assess, on a cell-by-cell basis, inversions and interstitial deletions whose small sizes are below the detection limits of conventional cytogenetic techniques.
KW - Chromosome aberrations
KW - F-ratio
KW - Inversions
KW - Ionizing radiation
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UR - http://www.scopus.com/inward/citedby.url?scp=85046678080&partnerID=8YFLogxK
U2 - 10.1016/j.mrgentox.2018.05.002
DO - 10.1016/j.mrgentox.2018.05.002
M3 - Article
JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
SN - 1383-5718
ER -