Radioactive microspheres (RM) are a standard method for the determination of microvascular blood flow (Qtnv). Colored microspheres (CM) are an attractive alternative, due to an unlimited storage time and lower cost of animal disposal. The purpose of this study was to compare RMs and CMs in the determination of Qmv in healthy sheep. Methods: Sheep (n = 90) were prepared with arterial, venous, and left atrial catheters. After recovery, 5 million CMs (n=60) or RMs (n=30) (diameter: 15 ±0.1 urn) were injected into the left atrium while reference blood was withdrawn. After sacrifice transmural tissue samples were harvested for determination of Qmv.Statistics: Student's l-test. Bg njjgl Data presented as mean ±SEM. Tissue TlM (ml/min/jT RM (ml/min/g) Differeiïce Trachea 0.13 ±0.04 0.12 ±0.02 Not SigniftcanT Lt. Ventricle 1.50±0.0g 1.46 + 0.08 Not Signifjcam neum 0.58 ±0.05 0.62 ±0.05 Not Significant' Colon 0.62 ±0.05 0.36 ±0.06 P = 0.02 Spleen 2.77 ±0.18 2.40 ±0.17 NÖTSignificant Pancreas 3.14 ±0.16 2.19 ±0.17 P = 0.005 Sk. Muscle 0.1610.02 0.1010.05 Not SigniflcanT Skin 0.08 ±0.02 0.06 ±0.00 Not Significant Kidney Conex 7.22 ±0.31 6.09 ±0.39 P = 0.03 Discussion: Both microsphere techniques provide a similar assessment of Qmv in the majority of tissues, however, Qmv in the colon, pancreas, and kidney cortex was significantly higher when determined with CMs.
|Original language||English (US)|
|State||Published - Dec 1 1996|
ASJC Scopus subject areas
- Molecular Biology