Radioactive vs. colored microspheres in the determination of regional blood flow in sheep

P. Schenans, H. Bone, George Kramer, V. Pollard, J. Meyer, L. Cindrick, D. Traber

Research output: Contribution to journalArticle

Abstract

Radioactive microspheres (RM) are a standard method for the determination of microvascular blood flow (Qtnv). Colored microspheres (CM) are an attractive alternative, due to an unlimited storage time and lower cost of animal disposal. The purpose of this study was to compare RMs and CMs in the determination of Qmv in healthy sheep. Methods: Sheep (n = 90) were prepared with arterial, venous, and left atrial catheters. After recovery, 5 million CMs (n=60) or RMs (n=30) (diameter: 15 ±0.1 urn) were injected into the left atrium while reference blood was withdrawn. After sacrifice transmural tissue samples were harvested for determination of Qmv.Statistics: Student's l-test. Bg njjgl Data presented as mean ±SEM. Tissue TlM (ml/min/jT RM (ml/min/g) Differeiïce Trachea 0.13 ±0.04 0.12 ±0.02 Not SigniftcanT Lt. Ventricle 1.50±0.0g 1.46 + 0.08 Not Signifjcam neum 0.58 ±0.05 0.62 ±0.05 Not Significant' Colon 0.62 ±0.05 0.36 ±0.06 P = 0.02 Spleen 2.77 ±0.18 2.40 ±0.17 NÖTSignificant Pancreas 3.14 ±0.16 2.19 ±0.17 P = 0.005 Sk. Muscle 0.1610.02 0.1010.05 Not SigniflcanT Skin 0.08 ±0.02 0.06 ±0.00 Not Significant Kidney Conex 7.22 ±0.31 6.09 ±0.39 P = 0.03 Discussion: Both microsphere techniques provide a similar assessment of Qmv in the majority of tissues, however, Qmv in the colon, pancreas, and kidney cortex was significantly higher when determined with CMs.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996

Fingerprint

Regional Blood Flow
Microspheres
blood flow
Sheep
Blood
sheep
pancreas
colon
Tissue
kidneys
Pancreas
Colon
skin (animal)
catheters
Kidney Cortex
Catheters
storage time
cortex
spleen
students

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Schenans, P., Bone, H., Kramer, G., Pollard, V., Meyer, J., Cindrick, L., & Traber, D. (1996). Radioactive vs. colored microspheres in the determination of regional blood flow in sheep. FASEB Journal, 10(3).

Radioactive vs. colored microspheres in the determination of regional blood flow in sheep. / Schenans, P.; Bone, H.; Kramer, George; Pollard, V.; Meyer, J.; Cindrick, L.; Traber, D.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

Schenans, P, Bone, H, Kramer, G, Pollard, V, Meyer, J, Cindrick, L & Traber, D 1996, 'Radioactive vs. colored microspheres in the determination of regional blood flow in sheep', FASEB Journal, vol. 10, no. 3.
Schenans, P. ; Bone, H. ; Kramer, George ; Pollard, V. ; Meyer, J. ; Cindrick, L. ; Traber, D. / Radioactive vs. colored microspheres in the determination of regional blood flow in sheep. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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AU - Schenans, P.

AU - Bone, H.

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AU - Traber, D.

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