Radioimmunoimaging of xenograft pancreatic cancer with 131I-monoclonal antibody P2

Zheng Yao Chong Zheng Yao, G. J. Poston, J. Ishizuka, Courtney Townsend, J. C. Thompson

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Monoclonal antibodies (McAbs) to pancreatic cancer were developed by fusing SP2/0 cells and splenocytes from Balb/c mice immunized with CH-2 cells. The specific binding rates of McAb P1 and P2 were 40.1 and 43.8%, respectively, shown by binding radioreactivity assay in vitro, which were in sharp contrast with those of control groups (p < 0.05). The biodistribution of radioiodinated McAb P2 was studied by measuring parameters of tumor- specific radioreactivity in nude mice bearing CH-2 tumors. The ratios of tumors to nontumors were all >2 at 48 h. The localization index of cancer and the ratio of tumor to pancreas were 4.05 and 4.16, respectively, at 72 h. Therefore, 131I-McAbs may be useful for radioimmunoimaging (RII) of pancreatic cancer. After intraperitoneal injection of 131I-McAb P2 into tumor-bearing nude mice, imaging of xenograft pancreatic cancer became increasingly distinct with the nonspecific background fading, especially in the period of 72-96 h. Examination of pancreatic cancer tissues by immunohistochemical methods revealed that McAb P2 was strongly positive (86%) in comparison with other tumors and normal tissues. The results demonstrated that clinical RII of pancreatic cancer was feasible with McAb P2.

Original languageEnglish (US)
Pages (from-to)289-294
Number of pages6
JournalPancreas
Volume8
Issue number3
StatePublished - 1993

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Keywords

  • Localization index
  • Monoclonal antibody
  • Pancreatic cancer
  • Radioimmunoimaging
  • Tumor/nontumor ratio
  • Xenograft tumor

ASJC Scopus subject areas

  • Endocrinology
  • Gastroenterology

Cite this

Chong Zheng Yao, Z. Y., Poston, G. J., Ishizuka, J., Townsend, C., & Thompson, J. C. (1993). Radioimmunoimaging of xenograft pancreatic cancer with 131I-monoclonal antibody P2. Pancreas, 8(3), 289-294.