Radioiodinated derivatives for steroid radioimmunoassay. Application to the radioimmunoassay of cortisol

Celso Gomez-Sanchez, Leon Milewich, O. Bryan Holland

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79 Scopus citations

Abstract

Gamma-emitting steroid tracers for use in the radioimmunoassay of steroids have a number of advantages over the more common tritiated tracers. The steroid derivatives aldosterone-3-(p-hydroxybenzoyl) hydrazone, aldosterone-3-(p-hydroxyphenylpropionyl)hydrazone, deoxycorticosterone-3-(p-hydroxyphenylpropionyl)hydrazone, and cortisol-3-(p-hydroxyphenylpropionyl)hydrazone were synthesized by a one-step procedure and iodinated ([125I]). To illustrate the usefulness of these derivatives, we describe the details of a cortisol radioimmunoassay. The use of the radioiodinated tracer appeared to increase the specificity of the antigen-antibody reaction when compared with [3H]cortisol. The methodology involved in the preparation of the steroid derivatives described above can be extended to other 3-oxo-4-ene-containing steroids, with the advantages of economy, simplicity, and versatility.

Original languageEnglish (US)
Pages (from-to)902-909
Number of pages8
JournalThe Journal of Laboratory and Clinical Medicine
Volume89
Issue number4
StatePublished - 1977

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ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

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