Radioiodinated derivatives for steroid radioimmunoassay. Application to the radioimmunoassay of cortisol

Celso Gomez-Sanchez, Leon Milewich, O. Bryan Holland

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Gamma-emitting steroid tracers for use in the radioimmunoassay of steroids have a number of advantages over the more common tritiated tracers. The steroid derivatives aldosterone-3-(p-hydroxybenzoyl) hydrazone, aldosterone-3-(p-hydroxyphenylpropionyl)hydrazone, deoxycorticosterone-3-(p-hydroxyphenylpropionyl)hydrazone, and cortisol-3-(p-hydroxyphenylpropionyl)hydrazone were synthesized by a one-step procedure and iodinated ([125I]). To illustrate the usefulness of these derivatives, we describe the details of a cortisol radioimmunoassay. The use of the radioiodinated tracer appeared to increase the specificity of the antigen-antibody reaction when compared with [3H]cortisol. The methodology involved in the preparation of the steroid derivatives described above can be extended to other 3-oxo-4-ene-containing steroids, with the advantages of economy, simplicity, and versatility.

Original languageEnglish (US)
Pages (from-to)902-909
Number of pages8
JournalThe Journal of Laboratory and Clinical Medicine
Volume89
Issue number4
StatePublished - 1977

Fingerprint

Hydrazones
Radioimmunoassay
Hydrocortisone
Steroids
Derivatives
Aldosterone
Antigen-antibody reactions
Antigen-Antibody Reactions
Desoxycorticosterone

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Radioiodinated derivatives for steroid radioimmunoassay. Application to the radioimmunoassay of cortisol. / Gomez-Sanchez, Celso; Milewich, Leon; Holland, O. Bryan.

In: The Journal of Laboratory and Clinical Medicine, Vol. 89, No. 4, 1977, p. 902-909.

Research output: Contribution to journalArticle

Gomez-Sanchez, Celso ; Milewich, Leon ; Holland, O. Bryan. / Radioiodinated derivatives for steroid radioimmunoassay. Application to the radioimmunoassay of cortisol. In: The Journal of Laboratory and Clinical Medicine. 1977 ; Vol. 89, No. 4. pp. 902-909.
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