Radioreceptorassay Of Opioid Peptides With 3H-Methionine Enkephalin And A Canine Limbic System Receptor Preparation

Danxia Liu

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A radioreceptor assay (RRA) method is described to detect opioid peptides that uses a canine limbic system P2 receptor preparation and 3H-methionine enkephalin as the displaced ligand. In our laboratory, 3H-ME is a useful probe to detect the presence of, and to facilitate the description of, opioid peptides in biological extracts. Those opioid receptor-active peaks are then studied subsequently in greater detail with immunoassay and mass spectrometry methods. A variety of peptide ligands including leucine enkephalin, beta-endorphin, Tyr-D-Ala-GIy-NMe-Phe-Gly-OL (“DAGO”), Tyr-D-Ser-Gly-Phe-Leu-Thr (“delta-receptor peptide”), and several dynorphins (1–7, 1–8, 1–9, 1–10, 1–17, and B) were used to further characterize the molecular specificity of that RRA system.

Original languageEnglish (US)
Pages (from-to)1865-1880
Number of pages16
JournalAnalytical Letters
Volume21
Issue number10
DOIs
StatePublished - Oct 1 1988

Keywords

  • 3H-methionine
  • enkephalin
  • limbic system
  • opioid
  • peptide
  • radioreceptorassay

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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