Ragweed Subpollen Particles of Respirable Size Activate Human Dendritic Cells

Kitti Pazmandi, Brahma V. Kumar, Krisztina Szabo, Istvan Boldogh, Arpad Szoor, Gyorgy Vereb, Agota Veres, Arpad Lanyi, Eva Rajnavolgyi, Attila Bacsi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Ragweed (Ambrosia artemisiifolia) pollen grains, which are generally considered too large to reach the lower respiratory tract, release subpollen particles (SPPs) of respirable size upon hydration. These SPPs contain allergenic proteins and functional NAD(P)H oxidases. In this study, we examined whether exposure to SPPs initiates the activation of human monocyte-derived dendritic cells (moDCs). We found that treatment with freshly isolated ragweed SPPs increased the intracellular levels of reactive oxygen species (ROS) in moDCs. Phagocytosis of SPPs by moDCs, as demonstrated by confocal laser-scanning microscopy, led to an up-regulation of the cell surface expression of CD40, CD80, CD86, and HLA-DQ and an increase in the production of IL-6, TNF-α, IL-8, and IL-10. Furthermore, SPP-treated moDCs had an increased capacity to stimulate the proliferation of naïve T cells. Co-culture of SPP-treated moDCs with allogeneic CD3+ pan-T cells resulted in increased secretion of IFN-γ and IL-17 by T cells of both allergic and non-allergic subjects, but induced the production of IL-4 exclusively from the T cells of allergic individuals. Addition of exogenous NADPH further increased, while heat-inactivation or pre-treatment with diphenyleneiodonium (DPI), an inhibitor of NADPH oxidases, strongly diminished, the ability of SPPs to induce phenotypic and functional changes in moDCs, indicating that these processes were mediated, at least partly, by the intrinsic NAD(P)H oxidase activity of SPPs. Collectively, our data suggest that inhaled ragweed SPPs are fully capable of activating dendritic cells (DCs) in the airways and SPPs' NAD(P)H oxidase activity is involved in initiation of adaptive immune responses against innocuous pollen proteins.

Original languageEnglish (US)
Article numbere52085
JournalPLoS One
Volume7
Issue number12
DOIs
StatePublished - Dec 14 2012

Fingerprint

Ambrosia
dendritic cells
Particle Size
Dendritic Cells
particle size
Monocytes
T-cells
NADPH Oxidase
monocytes
T-Lymphocytes
T-lymphocytes
Pollen
HLA-DQ Antigens
Interleukin-17
Adaptive Immunity
Coculture Techniques
Interleukin-8
NADP
Phagocytosis
Cell culture

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Pazmandi, K., Kumar, B. V., Szabo, K., Boldogh, I., Szoor, A., Vereb, G., ... Bacsi, A. (2012). Ragweed Subpollen Particles of Respirable Size Activate Human Dendritic Cells. PLoS One, 7(12), [e52085]. https://doi.org/10.1371/journal.pone.0052085

Ragweed Subpollen Particles of Respirable Size Activate Human Dendritic Cells. / Pazmandi, Kitti; Kumar, Brahma V.; Szabo, Krisztina; Boldogh, Istvan; Szoor, Arpad; Vereb, Gyorgy; Veres, Agota; Lanyi, Arpad; Rajnavolgyi, Eva; Bacsi, Attila.

In: PLoS One, Vol. 7, No. 12, e52085, 14.12.2012.

Research output: Contribution to journalArticle

Pazmandi, K, Kumar, BV, Szabo, K, Boldogh, I, Szoor, A, Vereb, G, Veres, A, Lanyi, A, Rajnavolgyi, E & Bacsi, A 2012, 'Ragweed Subpollen Particles of Respirable Size Activate Human Dendritic Cells', PLoS One, vol. 7, no. 12, e52085. https://doi.org/10.1371/journal.pone.0052085
Pazmandi, Kitti ; Kumar, Brahma V. ; Szabo, Krisztina ; Boldogh, Istvan ; Szoor, Arpad ; Vereb, Gyorgy ; Veres, Agota ; Lanyi, Arpad ; Rajnavolgyi, Eva ; Bacsi, Attila. / Ragweed Subpollen Particles of Respirable Size Activate Human Dendritic Cells. In: PLoS One. 2012 ; Vol. 7, No. 12.
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abstract = "Ragweed (Ambrosia artemisiifolia) pollen grains, which are generally considered too large to reach the lower respiratory tract, release subpollen particles (SPPs) of respirable size upon hydration. These SPPs contain allergenic proteins and functional NAD(P)H oxidases. In this study, we examined whether exposure to SPPs initiates the activation of human monocyte-derived dendritic cells (moDCs). We found that treatment with freshly isolated ragweed SPPs increased the intracellular levels of reactive oxygen species (ROS) in moDCs. Phagocytosis of SPPs by moDCs, as demonstrated by confocal laser-scanning microscopy, led to an up-regulation of the cell surface expression of CD40, CD80, CD86, and HLA-DQ and an increase in the production of IL-6, TNF-α, IL-8, and IL-10. Furthermore, SPP-treated moDCs had an increased capacity to stimulate the proliferation of na{\"i}ve T cells. Co-culture of SPP-treated moDCs with allogeneic CD3+ pan-T cells resulted in increased secretion of IFN-γ and IL-17 by T cells of both allergic and non-allergic subjects, but induced the production of IL-4 exclusively from the T cells of allergic individuals. Addition of exogenous NADPH further increased, while heat-inactivation or pre-treatment with diphenyleneiodonium (DPI), an inhibitor of NADPH oxidases, strongly diminished, the ability of SPPs to induce phenotypic and functional changes in moDCs, indicating that these processes were mediated, at least partly, by the intrinsic NAD(P)H oxidase activity of SPPs. Collectively, our data suggest that inhaled ragweed SPPs are fully capable of activating dendritic cells (DCs) in the airways and SPPs' NAD(P)H oxidase activity is involved in initiation of adaptive immune responses against innocuous pollen proteins.",
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