RANTES is a chemotactic and activating factor for human eosinophils

Rafeul Alam, Susan Stafford, Patricia Forsythe, Robert Harrison, Debra Faubion, Michael A. Lett-Brown, J. Andrew Grant

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Abstract

RANTES is a member of the 8-kDa cytokine family that has been shown to possess chemotactic activity for monocytes and CD4 T cells. In this study, we investigated whether RANTES could affect eosinophil chemotaxis and function. Peripheral blood eosinophils from blood donors were isolated on Percoll gradients to >98,% purity and then used for chemotaxis, flow cytometry, eosinophil cationic protein release assay, and survival assay. We found that RANTES is chemotactic for eosinophils at 10-9 to 10-8 M concentrations. RANTES elicited 65% of the chemotactic response to 10-7 M platelet-activating factor in all experiments. The mechanism of chemotaxis was investigated by studying the expression of adhesion molecules on eosinophils by flow cytometry. We found that RANTES up-regulated the expression of CD11 b/CD18 on eosinophils in a dose-dependent manner. In another set of experiments, purified eosinophils incubated with various concentrations of RANTES released eosinophil cationic protein as measured by a RIA. We also investigated the effect of RANTES on eosinophil density. Leukocytes were incubated in the presence or absence of RANTES, and the distribution of eosinophils on discontinuous Percoll gradients was then examined. We found that eosinophils became hypodense (<1.085) when incubated in RANTES. However, unlike IL-3, RANTES did not affect the survival of eosinophils in a 4-day culture system. Thus, we established that RANTES is a chemotactic and activating factor for eosinophils.

Original languageEnglish
Pages (from-to)3442-3447
Number of pages6
JournalJournal of Immunology
Volume150
Issue number8 PART 1
StatePublished - 1993

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Chemokine CCL5
Chemotactic Factors
Eosinophils
Chemotaxis
Eosinophil Cationic Protein
Flow Cytometry
Eosinophil Chemotactic Factors
Interleukin-3
Platelet Activating Factor
Blood Donors
Monocytes
Leukocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Alam, R., Stafford, S., Forsythe, P., Harrison, R., Faubion, D., Lett-Brown, M. A., & Grant, J. A. (1993). RANTES is a chemotactic and activating factor for human eosinophils. Journal of Immunology, 150(8 PART 1), 3442-3447.

RANTES is a chemotactic and activating factor for human eosinophils. / Alam, Rafeul; Stafford, Susan; Forsythe, Patricia; Harrison, Robert; Faubion, Debra; Lett-Brown, Michael A.; Grant, J. Andrew.

In: Journal of Immunology, Vol. 150, No. 8 PART 1, 1993, p. 3442-3447.

Research output: Contribution to journalArticle

Alam, R, Stafford, S, Forsythe, P, Harrison, R, Faubion, D, Lett-Brown, MA & Grant, JA 1993, 'RANTES is a chemotactic and activating factor for human eosinophils', Journal of Immunology, vol. 150, no. 8 PART 1, pp. 3442-3447.
Alam R, Stafford S, Forsythe P, Harrison R, Faubion D, Lett-Brown MA et al. RANTES is a chemotactic and activating factor for human eosinophils. Journal of Immunology. 1993;150(8 PART 1):3442-3447.
Alam, Rafeul ; Stafford, Susan ; Forsythe, Patricia ; Harrison, Robert ; Faubion, Debra ; Lett-Brown, Michael A. ; Grant, J. Andrew. / RANTES is a chemotactic and activating factor for human eosinophils. In: Journal of Immunology. 1993 ; Vol. 150, No. 8 PART 1. pp. 3442-3447.
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