Rapid, Affordable and Portable Medium-Throughput Molecular Device for Zika Virus

Kamfai Chan, Scott Weaver, Pui Yan Wong, Sherly Lie, Eryu Wang, Mathilde Guerbois, Siva Praneeth Vayugundla, Season Wong

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Zika virus (ZIKV) has gained global attention as an etiologic agent of fetal microcephaly and Guillain-Barré syndrome. Existing immuno-based rapid tests often fail to distinguish between Zika and related flaviviruses that are common in affected regions of Central and South Americas and the Caribbean. The US CDC and qualified state health department laboratories can perform the reverse transcription polymerase chain reaction (RT-PCR) ZIKV test using highly sophisticated instruments with long turnaround times. The preliminary results of a portable and low-cost molecular diagnostics system for ZIKV infection are reported here. In less than 15 minutes, this low-cost platform can automatically perform high quality RNA extraction from up to 12 ZIKV-spiked urine samples simultaneously. It can also perform reverse transcription recombinase polymerase amplification reaction (RT-RPA) in ≤15 minutes. The fluorescent signal produced from probe-based RT-RPA or RT-PCR assays can be monitored using LEDs and a smartphone camera. In addition, the RT-RPA and RT-PCR assays do not cross-react with dengue and chikungunya viral RNA. This low-cost system lacks complicated, sensitive and high cost components, making it suitable for resource-limited settings. It has the potential to offer simple sample-to-answer molecular diagnostics and can inform healthcare workers of patients' diagnosis promptly.

Original languageEnglish (US)
Article number38223
JournalScientific Reports
Volume6
DOIs
StatePublished - Dec 9 2016

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Reverse Transcription
Equipment and Supplies
Recombinases
Costs and Cost Analysis
Molecular Pathology
Polymerase Chain Reaction
Flavivirus
Central America
Microcephaly
Dengue
South America
Viral RNA
Centers for Disease Control and Prevention (U.S.)
Zika Virus
Urine
RNA
Delivery of Health Care
Health

ASJC Scopus subject areas

  • General

Cite this

Rapid, Affordable and Portable Medium-Throughput Molecular Device for Zika Virus. / Chan, Kamfai; Weaver, Scott; Wong, Pui Yan; Lie, Sherly; Wang, Eryu; Guerbois, Mathilde; Vayugundla, Siva Praneeth; Wong, Season.

In: Scientific Reports, Vol. 6, 38223, 09.12.2016.

Research output: Contribution to journalArticle

Chan, K, Weaver, S, Wong, PY, Lie, S, Wang, E, Guerbois, M, Vayugundla, SP & Wong, S 2016, 'Rapid, Affordable and Portable Medium-Throughput Molecular Device for Zika Virus', Scientific Reports, vol. 6, 38223. https://doi.org/10.1038/srep38223
Chan, Kamfai ; Weaver, Scott ; Wong, Pui Yan ; Lie, Sherly ; Wang, Eryu ; Guerbois, Mathilde ; Vayugundla, Siva Praneeth ; Wong, Season. / Rapid, Affordable and Portable Medium-Throughput Molecular Device for Zika Virus. In: Scientific Reports. 2016 ; Vol. 6.
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