Rapid and prolonged impairment of gut barrier function after thermal injury in mice

Tonyia Eaves-Pyles, J. Wesley Alexander

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Loss of gut barrier function after burn injury can be important in the pathogenesis of systemic infections and organ dysfunction. The purpose of this study was to determine how rapidly impairment of gut barrier function occurs after burn injury and how long it persists. BALB/c mice were gavaged with 1010111In-oxine-labeled Escherichia coli 3 h before inflicting a 20% total body surface area burn. They were then killed at 5, 15, 30, 60, 120, or 240 min post-burn. Additional mice were given a 20% or 30% burn injury and were randomized into eight groups, which were killed at either 4 h or 1, 2, 4, 7, 14, or 21 days post-burn. Each mouse was gavaged with 1010 111In-oxine-labeled E. coli 4 h before sacrifice to determine the magnitude of translocation. Gut barrier function was impaired as early as 5 min post-burn and was maximal by 4 h. Rapid improvement was observed by 24 h, followed by slow improvement, but with persistent abnormality through 21 days post-burn. Killing of translocated bacteria was impaired at 4 h and day 7 post-burn, according to the percentage of viable E. coli that remained alive in the tissues. The magnitude of gut dysfunction following burn injury is temporally related.

Original languageEnglish (US)
Pages (from-to)95-100
Number of pages6
Issue number2
StatePublished - Feb 1998
Externally publishedYes

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine


Dive into the research topics of 'Rapid and prolonged impairment of gut barrier function after thermal injury in mice'. Together they form a unique fingerprint.

Cite this