Rapid aqueous photo-polymerization route to polymer and polymer-composite hydrogel 3D inverted colloidal crystal scaffolds

Yuanfang Liu, Shaopeng Wang, Justin Krouse, Nicholas A. Kotov, Mohammad Eghtedari, Gracie Vargas, Massoud Motamedi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Successful regeneration of biological tissues in vitro requires the utilization of three-dimensional (3D) scaffolds that provide a near natural microenvironment for progenitor cells to grow, interact, replicate, and differentiate to form target tissues. In this work, a rapid aqueous photo-polymerization route was developed toward the fabrication of a variety of polymer hydrogel 3D inverted colloidal crystal (ICC) scaffolds having different physical and chemical properties. To demonstrate the versatility of this technique, a variety of polymer hydrogel ICC scaffolds were prepared, including (1) polyacrylamide (pAAM) scaffolds, (2) poly(2-hydroxyethyl methacrylate) (pHEMA) scaffolds, (3) poly(2-hydroxyethyl acrylate) (pHEA) scaffolds, and composite scaffolds including (4) pAAM-pHEMA scaffolds, (5) pHEMA-pMAETAC [poly(2-methacryloyloxy) trimethyl ammonium] scaffolds, and (6) pHEA-pMEATAC scaffolds. Templates for scaffolds incorporated both uniform sized (104 μm diameter) and nonuniform sized (100 ± 20 μm diameter) closely packed noncrosslinked poly(methyl methacrylate) beads. Human bone marrow stromal HS-5 cells were cultured on the six different types of scaffolds to demonstrate biocompatibility. Experimental results show that cells can remain viable in these scaffolds for at least 5 weeks. Of the six scaffolds, maximal cell adhesion and proliferation are obtained on the positively charged composite hydrogel pHEMA-pMEATAC and pHEA-pMAETAC scaffolds.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume83
Issue number1
DOIs
StatePublished - Oct 2007

Fingerprint

Photopolymerization
Hydrogel
Hydrogels
Scaffolds
Polymers
Crystals
Composite materials
Scaffolds (biology)
Polyacrylates
Tissue
Cell adhesion
Cell proliferation
Polymethyl Methacrylate
Polymethyl methacrylates
Biocompatibility
Ammonium Compounds
Chemical properties
Bone
Physical properties

Keywords

  • HS-5 human bone marrow stromal cells
  • Hydrogel scaffold
  • Inverted colloidal crystal
  • Photo-polymerization
  • Three-dimensional (3D)

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Rapid aqueous photo-polymerization route to polymer and polymer-composite hydrogel 3D inverted colloidal crystal scaffolds. / Liu, Yuanfang; Wang, Shaopeng; Krouse, Justin; Kotov, Nicholas A.; Eghtedari, Mohammad; Vargas, Gracie; Motamedi, Massoud.

In: Journal of Biomedical Materials Research - Part A, Vol. 83, No. 1, 10.2007, p. 1-9.

Research output: Contribution to journalArticle

@article{70868f8df5f14abc8fa85f5a56f878ec,
title = "Rapid aqueous photo-polymerization route to polymer and polymer-composite hydrogel 3D inverted colloidal crystal scaffolds",
abstract = "Successful regeneration of biological tissues in vitro requires the utilization of three-dimensional (3D) scaffolds that provide a near natural microenvironment for progenitor cells to grow, interact, replicate, and differentiate to form target tissues. In this work, a rapid aqueous photo-polymerization route was developed toward the fabrication of a variety of polymer hydrogel 3D inverted colloidal crystal (ICC) scaffolds having different physical and chemical properties. To demonstrate the versatility of this technique, a variety of polymer hydrogel ICC scaffolds were prepared, including (1) polyacrylamide (pAAM) scaffolds, (2) poly(2-hydroxyethyl methacrylate) (pHEMA) scaffolds, (3) poly(2-hydroxyethyl acrylate) (pHEA) scaffolds, and composite scaffolds including (4) pAAM-pHEMA scaffolds, (5) pHEMA-pMAETAC [poly(2-methacryloyloxy) trimethyl ammonium] scaffolds, and (6) pHEA-pMEATAC scaffolds. Templates for scaffolds incorporated both uniform sized (104 μm diameter) and nonuniform sized (100 ± 20 μm diameter) closely packed noncrosslinked poly(methyl methacrylate) beads. Human bone marrow stromal HS-5 cells were cultured on the six different types of scaffolds to demonstrate biocompatibility. Experimental results show that cells can remain viable in these scaffolds for at least 5 weeks. Of the six scaffolds, maximal cell adhesion and proliferation are obtained on the positively charged composite hydrogel pHEMA-pMEATAC and pHEA-pMAETAC scaffolds.",
keywords = "HS-5 human bone marrow stromal cells, Hydrogel scaffold, Inverted colloidal crystal, Photo-polymerization, Three-dimensional (3D)",
author = "Yuanfang Liu and Shaopeng Wang and Justin Krouse and Kotov, {Nicholas A.} and Mohammad Eghtedari and Gracie Vargas and Massoud Motamedi",
year = "2007",
month = "10",
doi = "10.1002/jbm.a.31199",
language = "English (US)",
volume = "83",
pages = "1--9",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Rapid aqueous photo-polymerization route to polymer and polymer-composite hydrogel 3D inverted colloidal crystal scaffolds

AU - Liu, Yuanfang

AU - Wang, Shaopeng

AU - Krouse, Justin

AU - Kotov, Nicholas A.

AU - Eghtedari, Mohammad

AU - Vargas, Gracie

AU - Motamedi, Massoud

PY - 2007/10

Y1 - 2007/10

N2 - Successful regeneration of biological tissues in vitro requires the utilization of three-dimensional (3D) scaffolds that provide a near natural microenvironment for progenitor cells to grow, interact, replicate, and differentiate to form target tissues. In this work, a rapid aqueous photo-polymerization route was developed toward the fabrication of a variety of polymer hydrogel 3D inverted colloidal crystal (ICC) scaffolds having different physical and chemical properties. To demonstrate the versatility of this technique, a variety of polymer hydrogel ICC scaffolds were prepared, including (1) polyacrylamide (pAAM) scaffolds, (2) poly(2-hydroxyethyl methacrylate) (pHEMA) scaffolds, (3) poly(2-hydroxyethyl acrylate) (pHEA) scaffolds, and composite scaffolds including (4) pAAM-pHEMA scaffolds, (5) pHEMA-pMAETAC [poly(2-methacryloyloxy) trimethyl ammonium] scaffolds, and (6) pHEA-pMEATAC scaffolds. Templates for scaffolds incorporated both uniform sized (104 μm diameter) and nonuniform sized (100 ± 20 μm diameter) closely packed noncrosslinked poly(methyl methacrylate) beads. Human bone marrow stromal HS-5 cells were cultured on the six different types of scaffolds to demonstrate biocompatibility. Experimental results show that cells can remain viable in these scaffolds for at least 5 weeks. Of the six scaffolds, maximal cell adhesion and proliferation are obtained on the positively charged composite hydrogel pHEMA-pMEATAC and pHEA-pMAETAC scaffolds.

AB - Successful regeneration of biological tissues in vitro requires the utilization of three-dimensional (3D) scaffolds that provide a near natural microenvironment for progenitor cells to grow, interact, replicate, and differentiate to form target tissues. In this work, a rapid aqueous photo-polymerization route was developed toward the fabrication of a variety of polymer hydrogel 3D inverted colloidal crystal (ICC) scaffolds having different physical and chemical properties. To demonstrate the versatility of this technique, a variety of polymer hydrogel ICC scaffolds were prepared, including (1) polyacrylamide (pAAM) scaffolds, (2) poly(2-hydroxyethyl methacrylate) (pHEMA) scaffolds, (3) poly(2-hydroxyethyl acrylate) (pHEA) scaffolds, and composite scaffolds including (4) pAAM-pHEMA scaffolds, (5) pHEMA-pMAETAC [poly(2-methacryloyloxy) trimethyl ammonium] scaffolds, and (6) pHEA-pMEATAC scaffolds. Templates for scaffolds incorporated both uniform sized (104 μm diameter) and nonuniform sized (100 ± 20 μm diameter) closely packed noncrosslinked poly(methyl methacrylate) beads. Human bone marrow stromal HS-5 cells were cultured on the six different types of scaffolds to demonstrate biocompatibility. Experimental results show that cells can remain viable in these scaffolds for at least 5 weeks. Of the six scaffolds, maximal cell adhesion and proliferation are obtained on the positively charged composite hydrogel pHEMA-pMEATAC and pHEA-pMAETAC scaffolds.

KW - HS-5 human bone marrow stromal cells

KW - Hydrogel scaffold

KW - Inverted colloidal crystal

KW - Photo-polymerization

KW - Three-dimensional (3D)

UR - http://www.scopus.com/inward/record.url?scp=34548712983&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548712983&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.31199

DO - 10.1002/jbm.a.31199

M3 - Article

C2 - 17335022

AN - SCOPUS:34548712983

VL - 83

SP - 1

EP - 9

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

IS - 1

ER -