Abstract: In this study we demonstrate that 50 mRS K+ stimulates the conversion of L‐[3H] arginine to L‐[3H] citrulline and that this effect is blocked by 10 μM AT‐nitro‐l‐arginine, a nitric oxide synthase inhibitor, and Ca2+‐free conditions. Amiloride (1 mM) and low Na+ conditions were used to test the possible involvement of the Na+‐Ca2+ exchanger. These treatments were without effect. The calcium channel blockers 10 mRS Mg2+, 100 μM Cd2+, and 10 mRS Co2+ also blocked the K+ response, suggesting the involvement of voltage‐dependent calcium channels (VDCCs). The specific VDCC involved seems to be the P type, as funnel‐web spider toxin blocked the response whereas 200 μMNi2+, 10 μM nifedipine, and 100 nMω‐conotoxin did not.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of neurochemistry|
|State||Published - Jan 1994|
- Calcium channel
- Frontal cortex.
- Funnel‐web spider toxin
- Nitric oxide synthase
- Potassium chloride
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience