Rapid-cycle PCR method to detect Bordetella pertussis that fulfills all consensus recommendations for use of PCR in diagnosis of pertussis

Keyphrases

• Pertussis 100%
• PCR Method 100%
• Bordetella Pertussis 100%
• Recommendations for Practice 100%
• Consensus Recommendations 100%
• Rapid Cycle 100%
• IS481 66%
• Bordetella 33%
• Actin 16%
• Nested PCR (nPCR) 16%
• Oligonucleotides 16%
• Specimen Preparation 16%
• Clinical Sensitivity 16%
• Clinical Specificities 16%
• Oligonucleotide Probes 16%
• Laboratory Diagnosis 16%
• Pertussis Infection 16%
• Microwell 16%
• Insertion Sequence 16%
• Clinical Definition 16%
• Internal Control 16%
• Analytical Sensitivity 16%
• Outer Membrane Porin 16%
• PCR Inhibition 16%
• Porin Gene 16%
• Bordetella Parapertussis 16%
• Probe Hybridization 16%
• Hybrid Detection 16%
• Parapertussis 16%
• Bordetella Bronchiseptica 16%
• Overlap Extension PCR 16%
• Analytical Specificity 16%

Biochemistry, Genetics and Molecular Biology

• Bordetella Pertussis 100%
• Bordetella 100%
• Oligonucleotide 50%
• Actin 50%
• Nested Polymerase Chain Reaction 50%
• Outer Membrane 50%
• Insertion Sequence 50%
• Oligonucleotide Probe 50%
• Hybridization Probe 50%
• Bordetella bronchiseptica 50%
• Porin 50%
• Bordetella Parapertussis 50%

Immunology and Microbiology

• Bordetella Pertussis 100%
• Pertussis 100%
• Bordetella 28%
• Actin 14%
• Outer Membrane 14%
• Nested Polymerase Chain Reaction 14%
• Oligonucleotide Probe 14%
• Gene Insertion Sequence 14%
• Porin 14%
• Bordetella bronchiseptica 14%
• Bordetella Parapertussis 14%