Rapid detection of poliovirus by reverse transcription and polymerase chain amplification

Application for differentiation between poliovirus and nonpoliovirus enteroviruses

R. Abraham, T. Chonmaitree, J. McCombs, B. Prabhakar, P. T. Lo Verde, P. L. Ogra

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

This report describes a rapid method of detection of poliovirus from viral isolates of clinical specimens using a single set of primers selected from the conserved 5' noncoding region of the poliovirus genome. Of the 144 clinical viral isolates examined, 81 were positive for polioviruses and 50 were positive for nonpoliovirus enteroviruses by tissue culture neutralization and infectivity. All 81 (100%) of the viral isolates identified as poliovirus by tissue culture infectivity were also positive by polymerase chain reaction. Of 50 nonpoliovirus enterovirus isolates found to be negative for poliovirus by tissue culture neutralization and infectivity, 48 were also negative by polymerase chain reaction. The high sensitivity (100%) and specificity (96%) of the primer set indicate that this assay has potential clinical applicability in the diagnosis of nonpoliovirus enterovirus infection.

Original languageEnglish (US)
Pages (from-to)395-399
Number of pages5
JournalJournal of Clinical Microbiology
Volume31
Issue number2
StatePublished - 1993

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Poliovirus
Enterovirus
Reverse Transcription
Enterovirus Infections
Polymerase Chain Reaction
Genome
Sensitivity and Specificity

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Rapid detection of poliovirus by reverse transcription and polymerase chain amplification : Application for differentiation between poliovirus and nonpoliovirus enteroviruses. / Abraham, R.; Chonmaitree, T.; McCombs, J.; Prabhakar, B.; Lo Verde, P. T.; Ogra, P. L.

In: Journal of Clinical Microbiology, Vol. 31, No. 2, 1993, p. 395-399.

Research output: Contribution to journalArticle

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