Rapid detection of viruses of the tick-borne encephalitis virus complex by RT-PCR of viral RNA

J. E. Whitbya, H. Ni, H. E. Whitby, A. D. Jennings, L. M. Bradley, J. M. Lee, G. Lloyd, J. R. Stephenson, A. D.T. Barrett

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Studies were performed to identify a pair of primers, specific for the tick-borne encephalitis (TBE) virus complex of the Flaviviridae, with which to develop a rapid and specific identification system based on reverse transcription and the polymerase chain reaction (RT-PCR). The specificity of a putative primer pair was examined by RT-PCR of representative viruses from other antigenic complexes of the Flaviviridae and by computer sequence homology checks. All viruses of the TBE complex tested, with a single exception, were identified by RT-PCR using the identified primer pair. Accumulated data suggest that one of the putative primers identified in these studies may have flavivirus group specificity. The advantages of such a primer in the development of identification systems for all virus complexes of the Flaviviridae is discussed.

Original languageEnglish (US)
Pages (from-to)103-114
Number of pages12
JournalJournal of Virological Methods
Volume45
Issue number1
DOIs
StatePublished - Nov 1993

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Keywords

  • Probe
  • RT-PCR
  • Tick-borne encephalitis virus

ASJC Scopus subject areas

  • Virology

Cite this

Whitbya, J. E., Ni, H., Whitby, H. E., Jennings, A. D., Bradley, L. M., Lee, J. M., Lloyd, G., Stephenson, J. R., & Barrett, A. D. T. (1993). Rapid detection of viruses of the tick-borne encephalitis virus complex by RT-PCR of viral RNA. Journal of Virological Methods, 45(1), 103-114. https://doi.org/10.1016/0166-0934(93)90144-G