Rapid detection of viruses of the tick-borne encephalitis virus complex by RT-PCR of viral RNA

J. E. Whitbya, H. Ni, H. E. Whitby, A. D. Jennings, L. M. Bradley, J. M. Lee, G. Lloyd, J. R. Stephenson, Alan Barrett

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Studies were performed to identify a pair of primers, specific for the tick-borne encephalitis (TBE) virus complex of the Flaviviridae, with which to develop a rapid and specific identification system based on reverse transcription and the polymerase chain reaction (RT-PCR). The specificity of a putative primer pair was examined by RT-PCR of representative viruses from other antigenic complexes of the Flaviviridae and by computer sequence homology checks. All viruses of the TBE complex tested, with a single exception, were identified by RT-PCR using the identified primer pair. Accumulated data suggest that one of the putative primers identified in these studies may have flavivirus group specificity. The advantages of such a primer in the development of identification systems for all virus complexes of the Flaviviridae is discussed.

Original languageEnglish (US)
Pages (from-to)103-114
Number of pages12
JournalJournal of Virological Methods
Volume45
Issue number1
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Flaviviridae
Tick-Borne Encephalitis Viruses
Viral RNA
Reverse Transcription
Viruses
Polymerase Chain Reaction
Flavivirus
Sequence Homology

Keywords

  • Probe
  • RT-PCR
  • Tick-borne encephalitis virus

ASJC Scopus subject areas

  • Virology

Cite this

Rapid detection of viruses of the tick-borne encephalitis virus complex by RT-PCR of viral RNA. / Whitbya, J. E.; Ni, H.; Whitby, H. E.; Jennings, A. D.; Bradley, L. M.; Lee, J. M.; Lloyd, G.; Stephenson, J. R.; Barrett, Alan.

In: Journal of Virological Methods, Vol. 45, No. 1, 1993, p. 103-114.

Research output: Contribution to journalArticle

Whitbya, JE, Ni, H, Whitby, HE, Jennings, AD, Bradley, LM, Lee, JM, Lloyd, G, Stephenson, JR & Barrett, A 1993, 'Rapid detection of viruses of the tick-borne encephalitis virus complex by RT-PCR of viral RNA', Journal of Virological Methods, vol. 45, no. 1, pp. 103-114. https://doi.org/10.1016/0166-0934(93)90144-G
Whitbya, J. E. ; Ni, H. ; Whitby, H. E. ; Jennings, A. D. ; Bradley, L. M. ; Lee, J. M. ; Lloyd, G. ; Stephenson, J. R. ; Barrett, Alan. / Rapid detection of viruses of the tick-borne encephalitis virus complex by RT-PCR of viral RNA. In: Journal of Virological Methods. 1993 ; Vol. 45, No. 1. pp. 103-114.
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