Rapid identification of Ebola virus and related filoviruses in fluid specimens using indirect immunoelectron microscopy

Thomas Geisbert, J. B. Rhoderick, P. B. Jahrling

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Recent filoviral outbreaks in animal primates have raised public awareness of the potential for filoviruses to become a public health concern; methods that efficiently identify these viruses are therefore of high priority. An indirect immunoelectron microscopy method, which uses homologous guinea pig polyclonal antiserum, successfully identified Ebola-related (Reston) virus particles in serum and tissue culture fluid specimens with infectivity titres of 300 plaque forming units (pfu) per ml or more. The sensitivity of this procedure is sufficient to show virus in most acute phase sera, and is equal to that of the antigen capture enzyme linked immunosorbent assay (ELISA). The immunoelectron microscopy fluid technique can differentiate among antigenically distinct filoviruses in less than three hours. It should be valuable in the rapid diagnosis of potential filoviral infections.

Original languageEnglish (US)
Pages (from-to)521-522
Number of pages2
JournalJournal of Clinical Pathology
Volume44
Issue number6
StatePublished - 1991
Externally publishedYes

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Ebolavirus
Immunoelectron Microscopy
Viruses
Serum
Virion
Primates
Disease Outbreaks
Immune Sera
Guinea Pigs
Public Health
Enzyme-Linked Immunosorbent Assay
Antigens
Infection

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Rapid identification of Ebola virus and related filoviruses in fluid specimens using indirect immunoelectron microscopy. / Geisbert, Thomas; Rhoderick, J. B.; Jahrling, P. B.

In: Journal of Clinical Pathology, Vol. 44, No. 6, 1991, p. 521-522.

Research output: Contribution to journalArticle

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