Rapid identification of flaviviruses based on conserved NS5 gene sequences

L. Fulop, Alan Barrett, R. Phillpotts, K. Martin, D. Leslie, R. W. Titball

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Two conserved regions in the sequence of the NS5 gene of Flaviviruses were identified. Primers were designed from the consensus sequence of these regions and were used in a reverse transcription/polymerase chain reaction (RT/PCR) to amplify a region of the central european tick-borne encephalitis virus Kumlinge NS5 gene. The authenticity of the amplified fragment was confirmed by nucleotide sequencing. A band of the expected size was also obtained when this RT/PCR was applied to 13 other flaviviral RNAs. This method may be useful for characterisation of the NS5 genes of flaviviruses and as a potential pan-flavivirus diagnostic tool.

Original languageEnglish (US)
Pages (from-to)179-188
Number of pages10
JournalJournal of Virological Methods
Volume44
Issue number2-3
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Flavivirus
Tick-Borne Encephalitis
Reverse Transcription
Genes
Tick-Borne Encephalitis Viruses
Polymerase Chain Reaction
Consensus Sequence
Nucleotides
RNA

Keywords

  • Flavivirus
  • NS5 gene
  • RT/PCR

ASJC Scopus subject areas

  • Virology

Cite this

Rapid identification of flaviviruses based on conserved NS5 gene sequences. / Fulop, L.; Barrett, Alan; Phillpotts, R.; Martin, K.; Leslie, D.; Titball, R. W.

In: Journal of Virological Methods, Vol. 44, No. 2-3, 1993, p. 179-188.

Research output: Contribution to journalArticle

Fulop, L. ; Barrett, Alan ; Phillpotts, R. ; Martin, K. ; Leslie, D. ; Titball, R. W. / Rapid identification of flaviviruses based on conserved NS5 gene sequences. In: Journal of Virological Methods. 1993 ; Vol. 44, No. 2-3. pp. 179-188.
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