Rapid molecular strategy for orbivirus detection and characterization

Gustavo Palacios, Chris Cowled, Ana V. Bussetti, Nazir Savji, Richard Weir, Ivan Wick, Amelia Travassos Da Rosa, Charles H. Calisher, Robert B. Tesh, David Boyle, W. Ian Lipkin

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.

Original languageEnglish (US)
Pages (from-to)2314-2317
Number of pages4
JournalJournal of Clinical Microbiology
Volume49
Issue number6
DOIs
StatePublished - Jun 2011

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Orbivirus
Gene Targeting
Host Specificity
Reverse Transcription
Sequence Analysis
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Palacios, G., Cowled, C., Bussetti, A. V., Savji, N., Weir, R., Wick, I., ... Lipkin, W. I. (2011). Rapid molecular strategy for orbivirus detection and characterization. Journal of Clinical Microbiology, 49(6), 2314-2317. https://doi.org/10.1128/JCM.00337-11

Rapid molecular strategy for orbivirus detection and characterization. / Palacios, Gustavo; Cowled, Chris; Bussetti, Ana V.; Savji, Nazir; Weir, Richard; Wick, Ivan; Da Rosa, Amelia Travassos; Calisher, Charles H.; Tesh, Robert B.; Boyle, David; Lipkin, W. Ian.

In: Journal of Clinical Microbiology, Vol. 49, No. 6, 06.2011, p. 2314-2317.

Research output: Contribution to journalArticle

Palacios, G, Cowled, C, Bussetti, AV, Savji, N, Weir, R, Wick, I, Da Rosa, AT, Calisher, CH, Tesh, RB, Boyle, D & Lipkin, WI 2011, 'Rapid molecular strategy for orbivirus detection and characterization', Journal of Clinical Microbiology, vol. 49, no. 6, pp. 2314-2317. https://doi.org/10.1128/JCM.00337-11
Palacios G, Cowled C, Bussetti AV, Savji N, Weir R, Wick I et al. Rapid molecular strategy for orbivirus detection and characterization. Journal of Clinical Microbiology. 2011 Jun;49(6):2314-2317. https://doi.org/10.1128/JCM.00337-11
Palacios, Gustavo ; Cowled, Chris ; Bussetti, Ana V. ; Savji, Nazir ; Weir, Richard ; Wick, Ivan ; Da Rosa, Amelia Travassos ; Calisher, Charles H. ; Tesh, Robert B. ; Boyle, David ; Lipkin, W. Ian. / Rapid molecular strategy for orbivirus detection and characterization. In: Journal of Clinical Microbiology. 2011 ; Vol. 49, No. 6. pp. 2314-2317.
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