Rapid molecular strategy for orbivirus detection and characterization

Gustavo Palacios, Chris Cowled, Ana V. Bussetti, Nazir Savji, Richard Weir, Ivan Wick, Amelia Travassos Da Rosa, Charles H. Calisher, Robert B. Tesh, David Boyle, W. Ian Lipkin

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.

Original languageEnglish (US)
Pages (from-to)2314-2317
Number of pages4
JournalJournal of Clinical Microbiology
Volume49
Issue number6
DOIs
StatePublished - Jun 1 2011

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ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Palacios, G., Cowled, C., Bussetti, A. V., Savji, N., Weir, R., Wick, I., Da Rosa, A. T., Calisher, C. H., Tesh, R. B., Boyle, D., & Lipkin, W. I. (2011). Rapid molecular strategy for orbivirus detection and characterization. Journal of Clinical Microbiology, 49(6), 2314-2317. https://doi.org/10.1128/JCM.00337-11