Rapid molecular strategy for orbivirus detection and characterization

Gustavo Palacios; Chris Cowled; Ana V. Bussetti; Nazir Savji; Richard Weir; Ivan Wick; Amelia Travassos Da Rosa; Charles H. Calisher; Robert B. Tesh; David Boyle; W. Ian Lipkin

doi:10.1128/JCM.00337-11

Rapid molecular strategy for orbivirus detection and characterization

Gustavo Palacios
, Chris Cowled
, Ana V. Bussetti
, Nazir Savji
, Richard Weir
, Ivan Wick
, Amelia Travassos Da Rosa
, Charles H. Calisher
, Robert B. Tesh
, David Boyle
, W. Ian Lipkin

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.

Original language	English (US)
Pages (from-to)	2314-2317
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	49
Issue number	6
DOIs	https://doi.org/10.1128/JCM.00337-11
State	Published - Jun 2011
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.00337-11

Cite this

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abstract = "Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.",

author = "Gustavo Palacios and Chris Cowled and Bussetti, \{Ana V.\} and Nazir Savji and Richard Weir and Ivan Wick and \{Da Rosa\}, \{Amelia Travassos\} and Calisher, \{Charles H.\} and Tesh, \{Robert B.\} and David Boyle and Lipkin, \{W. Ian\}",

year = "2011",

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AU - Palacios, Gustavo

AU - Cowled, Chris

AU - Bussetti, Ana V.

AU - Savji, Nazir

AU - Weir, Richard

AU - Wick, Ivan

AU - Da Rosa, Amelia Travassos

AU - Calisher, Charles H.

AU - Tesh, Robert B.

AU - Boyle, David

AU - Lipkin, W. Ian

PY - 2011/6

Y1 - 2011/6

N2 - Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.

AB - Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.

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U2 - 10.1128/JCM.00337-11

DO - 10.1128/JCM.00337-11

M3 - Article

C2 - 21450948

AN - SCOPUS:79957483592

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SP - 2314

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JO - Journal of Clinical Microbiology

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ER -

Rapid molecular strategy for orbivirus detection and characterization

Abstract

ASJC Scopus subject areas

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