Rapid neurite formation in a human cortical neuronal cell line

K. J. Dunn, J. R. Perez-Polo, T. G. Wood

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Abstract

The subclone HCN-1 was derived from parental cell lines from cortical tissue of a patient with unilateral megalencephaly growth and immunochemistry staining characteristics. As we and others have shown, HCN-1A cells can be induced to differentiate to a neuronal-like morphology. HCN-1A cells stain positively for neurofilament, neuron-specific enolase and the low-affinity neurotrophin receptors, p75(NGFR), but not for myelin basic protein, S-100, or glial fibrillary acidic protein (GFAP). HCN-1A cells also stain positively for gamma-aminobutyric acid and glutamate. In the present study, we examine the effects of cell density on the requirements for efficient induction of differentiation of HCN-1A cells and analyze the time course of this induction and its reversion. We also characterize the changes in cytoskeletal proteins of HCN-1A cells in response to their differentiation neuronal phenotype.

Original languageEnglish (US)
Pages (from-to)61-68
Number of pages8
JournalInternational Journal of Developmental Neuroscience
Volume14
Issue number1
DOIs
StatePublished - Feb 1996

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ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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