Rapid neurite formation in a human cortical neuronal cell line

K. J. Dunn, J. R. Perez-Polo, Thomas Wood

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The subclone HCN-1 was derived from parental cell lines from cortical tissue of a patient with unilateral megalencephaly growth and immunochemistry staining characteristics. As we and others have shown, HCN-1A cells can be induced to differentiate to a neuronal-like morphology. HCN-1A cells stain positively for neurofilament, neuron-specific enolase and the low-affinity neurotrophin receptors, p75(NGFR), but not for myelin basic protein, S-100, or glial fibrillary acidic protein (GFAP). HCN-1A cells also stain positively for gamma-aminobutyric acid and glutamate. In the present study, we examine the effects of cell density on the requirements for efficient induction of differentiation of HCN-1A cells and analyze the time course of this induction and its reversion. We also characterize the changes in cytoskeletal proteins of HCN-1A cells in response to their differentiation neuronal phenotype.

Original languageEnglish (US)
Pages (from-to)61-68
Number of pages8
JournalInternational Journal of Developmental Neuroscience
Volume14
Issue number1
DOIs
StatePublished - Feb 1996

Fingerprint

Neurites
Cell Line
Coloring Agents
Immunochemistry
Nerve Growth Factor Receptor
Cytoskeletal Proteins
Myelin Basic Protein
Intermediate Filaments
Phosphopyruvate Hydratase
Glial Fibrillary Acidic Protein
Protein S
gamma-Aminobutyric Acid
Glutamic Acid
Cell Count
Staining and Labeling
Phenotype
Growth

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Rapid neurite formation in a human cortical neuronal cell line. / Dunn, K. J.; Perez-Polo, J. R.; Wood, Thomas.

In: International Journal of Developmental Neuroscience, Vol. 14, No. 1, 02.1996, p. 61-68.

Research output: Contribution to journalArticle

Dunn, K. J. ; Perez-Polo, J. R. ; Wood, Thomas. / Rapid neurite formation in a human cortical neuronal cell line. In: International Journal of Developmental Neuroscience. 1996 ; Vol. 14, No. 1. pp. 61-68.
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