Rat trehalase: cDNA cloning and mRNA expression in adult rat tissues and during intestinal ontogeny

Thomas J. Oesterreicher, Nanda N. Nanthakumar, John Winston, Susan J. Henning

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A partial rat trehalase cDNA has been cloned and used to examine trehalase mRNA expression. Northern blotting with total RNA from 11 adult rat tissues showed a trehalase transcript only in small intestine, where it was abundant in proximal regions but declined steeply toward the ileum. During development, trehalase mRNA was not detectable in jejunum until postnatal day 19 and then increased markedly through day 25. Modest levels of trehalase mRNA were induced precociously by administration of dexamethasone, with increasing responsiveness evident between the first and second postnatal weeks. In contrast, analysis of sucrase-isomaltase mRNA on the same blots showed maximal induction at both ages. In adrenalectomized animals, the ontogenic increase of trehalase mRNA began as usual but proceeded more slowly than in control animals. Overall, trehalase mRNA expression in the rat displayed both similarities and differences compared with rabbit. Moreover, the differences revealed in glucocorticoid responsiveness of trehalase mRNA and sucrase-isomaltase mRNA suggest that the actions of these hormones on the developing intestine may be more complex than previously recognized.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume274
Issue number5 43-5
StatePublished - May 1998
Externally publishedYes

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Trehalase
Organism Cloning
Complementary DNA
Messenger RNA
Oligo-1,6-Glucosidase
Sucrase
Jejunum
Ileum
Northern Blotting
Dexamethasone
Glucocorticoids
Small Intestine
Intestines
Hormones
RNA
Rabbits

Keywords

  • Adrenalectomy
  • Cephalocaudal gradient
  • Glucocorticoid
  • Sequence analysis

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Rat trehalase : cDNA cloning and mRNA expression in adult rat tissues and during intestinal ontogeny. / Oesterreicher, Thomas J.; Nanthakumar, Nanda N.; Winston, John; Henning, Susan J.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 274, No. 5 43-5, 05.1998.

Research output: Contribution to journalArticle

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