Rates of glucose uptake in adipose tissue and muscle in vivo after a mixed meal in women with morbid obesity

Panayota Mitrou, Eleni Boutati, Vaia Lambadiari, Eirini Maratou, Alexandros Papakonstantinou, Vasiliki Komesidou, Labros Sidossis, Nikolaos Tountas, Nikolaos Katsilambros, Theofanis Economopoulos, Sotirios A. Raptis, George Dimitriadis

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background and Aims: Although whole-body insulin resistance in obesity is established, information on insulin action in peripheral tissues, especially adipose tissue (AD), is limited. This study was undertaken in morbid obesity to investigate insulin action on glucose disposal in AD and muscle (M). Subjects and Methods: A meal was given to 30 obese (age 34 ± 1 yr, body mass index 47 ± 1 kg/m2) and 10 nonobese women (age 39 ± 4 yr, body mass index 23 ± 1 kg/m2). Samples for glucose and insulin were taken for 360 min from veins draining the abdominal subcutaneous AD and forearm muscles and from the radial artery. Blood flow (BF) was measured in AD (133Xe) and M (plethysmography). Results: The area under the curve divided by time (AUC0-360min/360min) in obese vs. nonobese was as follows: 1) arterial glucose was similar 6.04 ± 0.2 vs. 5.67 ± 0.1 mM), but insulin was increased (65.5 ± 6.6 vs. 28.7 ± 1.7 mU/liter, P = 0.0004); 2) BF was decreased (3 ± 0.2 vs. 4.4 ± 0.3 ml/min per 100 ml tissue in M, P = 0.002 and 1.8 ± 0.1 vs. 3.7 ± 0.3 ml/min per 100 ml tissue in AD, P < 0.0001); 3) glucose uptake was decreased (0.9 ± 0.1 vs. 2.3 ± 0.4 μmol/min per 100 ml tissue in M, P = 0.002 and 0.45 ± 0.1 vs. 1.1 ± 0.17 μmol/min per 100 ml tissue in AD, P = 0.01); 4) fractional glucose extraction was decreased in M (5 ± 1 vs. 9 ± 1%, P = 0.03), but was similar in AD (3 ± 1 vs. 3.6 ± 1.4%); 5) glucose uptake (per total fat mass) was increased (0.275 ± 0.04 vs. 0.12 ± 0.02 mmol/min, P = 0.027). Conclusion: In morbid obesity, the sensitivity of glucose metabolism to insulin is impaired in M, due to defects in insulin-stimulated glucose use and decreased BF, and in AD, at least in part, due to decreased BF. However, increased total fat mass provides a sink for the excess of glucose and compensates for insulin resistance.

Original languageEnglish (US)
Pages (from-to)2958-2961
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume94
Issue number8
DOIs
StatePublished - Aug 2009
Externally publishedYes

Fingerprint

Morbid Obesity
Meals
Muscle
Adipose Tissue
Tissue
Glucose
Muscles
Insulin
Blood
Insulin Resistance
Body Mass Index
Fats
Plethysmography
Radial Artery
Forearm
Metabolism
Area Under Curve
Veins
Obesity
Defects

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism

Cite this

Mitrou, P., Boutati, E., Lambadiari, V., Maratou, E., Papakonstantinou, A., Komesidou, V., ... Dimitriadis, G. (2009). Rates of glucose uptake in adipose tissue and muscle in vivo after a mixed meal in women with morbid obesity. Journal of Clinical Endocrinology and Metabolism, 94(8), 2958-2961. https://doi.org/10.1210/jc.2008-2297

Rates of glucose uptake in adipose tissue and muscle in vivo after a mixed meal in women with morbid obesity. / Mitrou, Panayota; Boutati, Eleni; Lambadiari, Vaia; Maratou, Eirini; Papakonstantinou, Alexandros; Komesidou, Vasiliki; Sidossis, Labros; Tountas, Nikolaos; Katsilambros, Nikolaos; Economopoulos, Theofanis; Raptis, Sotirios A.; Dimitriadis, George.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 94, No. 8, 08.2009, p. 2958-2961.

Research output: Contribution to journalArticle

Mitrou, P, Boutati, E, Lambadiari, V, Maratou, E, Papakonstantinou, A, Komesidou, V, Sidossis, L, Tountas, N, Katsilambros, N, Economopoulos, T, Raptis, SA & Dimitriadis, G 2009, 'Rates of glucose uptake in adipose tissue and muscle in vivo after a mixed meal in women with morbid obesity', Journal of Clinical Endocrinology and Metabolism, vol. 94, no. 8, pp. 2958-2961. https://doi.org/10.1210/jc.2008-2297
Mitrou, Panayota ; Boutati, Eleni ; Lambadiari, Vaia ; Maratou, Eirini ; Papakonstantinou, Alexandros ; Komesidou, Vasiliki ; Sidossis, Labros ; Tountas, Nikolaos ; Katsilambros, Nikolaos ; Economopoulos, Theofanis ; Raptis, Sotirios A. ; Dimitriadis, George. / Rates of glucose uptake in adipose tissue and muscle in vivo after a mixed meal in women with morbid obesity. In: Journal of Clinical Endocrinology and Metabolism. 2009 ; Vol. 94, No. 8. pp. 2958-2961.
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title = "Rates of glucose uptake in adipose tissue and muscle in vivo after a mixed meal in women with morbid obesity",
abstract = "Background and Aims: Although whole-body insulin resistance in obesity is established, information on insulin action in peripheral tissues, especially adipose tissue (AD), is limited. This study was undertaken in morbid obesity to investigate insulin action on glucose disposal in AD and muscle (M). Subjects and Methods: A meal was given to 30 obese (age 34 ± 1 yr, body mass index 47 ± 1 kg/m2) and 10 nonobese women (age 39 ± 4 yr, body mass index 23 ± 1 kg/m2). Samples for glucose and insulin were taken for 360 min from veins draining the abdominal subcutaneous AD and forearm muscles and from the radial artery. Blood flow (BF) was measured in AD (133Xe) and M (plethysmography). Results: The area under the curve divided by time (AUC0-360min/360min) in obese vs. nonobese was as follows: 1) arterial glucose was similar 6.04 ± 0.2 vs. 5.67 ± 0.1 mM), but insulin was increased (65.5 ± 6.6 vs. 28.7 ± 1.7 mU/liter, P = 0.0004); 2) BF was decreased (3 ± 0.2 vs. 4.4 ± 0.3 ml/min per 100 ml tissue in M, P = 0.002 and 1.8 ± 0.1 vs. 3.7 ± 0.3 ml/min per 100 ml tissue in AD, P < 0.0001); 3) glucose uptake was decreased (0.9 ± 0.1 vs. 2.3 ± 0.4 μmol/min per 100 ml tissue in M, P = 0.002 and 0.45 ± 0.1 vs. 1.1 ± 0.17 μmol/min per 100 ml tissue in AD, P = 0.01); 4) fractional glucose extraction was decreased in M (5 ± 1 vs. 9 ± 1{\%}, P = 0.03), but was similar in AD (3 ± 1 vs. 3.6 ± 1.4{\%}); 5) glucose uptake (per total fat mass) was increased (0.275 ± 0.04 vs. 0.12 ± 0.02 mmol/min, P = 0.027). Conclusion: In morbid obesity, the sensitivity of glucose metabolism to insulin is impaired in M, due to defects in insulin-stimulated glucose use and decreased BF, and in AD, at least in part, due to decreased BF. However, increased total fat mass provides a sink for the excess of glucose and compensates for insulin resistance.",
author = "Panayota Mitrou and Eleni Boutati and Vaia Lambadiari and Eirini Maratou and Alexandros Papakonstantinou and Vasiliki Komesidou and Labros Sidossis and Nikolaos Tountas and Nikolaos Katsilambros and Theofanis Economopoulos and Raptis, {Sotirios A.} and George Dimitriadis",
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T1 - Rates of glucose uptake in adipose tissue and muscle in vivo after a mixed meal in women with morbid obesity

AU - Mitrou, Panayota

AU - Boutati, Eleni

AU - Lambadiari, Vaia

AU - Maratou, Eirini

AU - Papakonstantinou, Alexandros

AU - Komesidou, Vasiliki

AU - Sidossis, Labros

AU - Tountas, Nikolaos

AU - Katsilambros, Nikolaos

AU - Economopoulos, Theofanis

AU - Raptis, Sotirios A.

AU - Dimitriadis, George

PY - 2009/8

Y1 - 2009/8

N2 - Background and Aims: Although whole-body insulin resistance in obesity is established, information on insulin action in peripheral tissues, especially adipose tissue (AD), is limited. This study was undertaken in morbid obesity to investigate insulin action on glucose disposal in AD and muscle (M). Subjects and Methods: A meal was given to 30 obese (age 34 ± 1 yr, body mass index 47 ± 1 kg/m2) and 10 nonobese women (age 39 ± 4 yr, body mass index 23 ± 1 kg/m2). Samples for glucose and insulin were taken for 360 min from veins draining the abdominal subcutaneous AD and forearm muscles and from the radial artery. Blood flow (BF) was measured in AD (133Xe) and M (plethysmography). Results: The area under the curve divided by time (AUC0-360min/360min) in obese vs. nonobese was as follows: 1) arterial glucose was similar 6.04 ± 0.2 vs. 5.67 ± 0.1 mM), but insulin was increased (65.5 ± 6.6 vs. 28.7 ± 1.7 mU/liter, P = 0.0004); 2) BF was decreased (3 ± 0.2 vs. 4.4 ± 0.3 ml/min per 100 ml tissue in M, P = 0.002 and 1.8 ± 0.1 vs. 3.7 ± 0.3 ml/min per 100 ml tissue in AD, P < 0.0001); 3) glucose uptake was decreased (0.9 ± 0.1 vs. 2.3 ± 0.4 μmol/min per 100 ml tissue in M, P = 0.002 and 0.45 ± 0.1 vs. 1.1 ± 0.17 μmol/min per 100 ml tissue in AD, P = 0.01); 4) fractional glucose extraction was decreased in M (5 ± 1 vs. 9 ± 1%, P = 0.03), but was similar in AD (3 ± 1 vs. 3.6 ± 1.4%); 5) glucose uptake (per total fat mass) was increased (0.275 ± 0.04 vs. 0.12 ± 0.02 mmol/min, P = 0.027). Conclusion: In morbid obesity, the sensitivity of glucose metabolism to insulin is impaired in M, due to defects in insulin-stimulated glucose use and decreased BF, and in AD, at least in part, due to decreased BF. However, increased total fat mass provides a sink for the excess of glucose and compensates for insulin resistance.

AB - Background and Aims: Although whole-body insulin resistance in obesity is established, information on insulin action in peripheral tissues, especially adipose tissue (AD), is limited. This study was undertaken in morbid obesity to investigate insulin action on glucose disposal in AD and muscle (M). Subjects and Methods: A meal was given to 30 obese (age 34 ± 1 yr, body mass index 47 ± 1 kg/m2) and 10 nonobese women (age 39 ± 4 yr, body mass index 23 ± 1 kg/m2). Samples for glucose and insulin were taken for 360 min from veins draining the abdominal subcutaneous AD and forearm muscles and from the radial artery. Blood flow (BF) was measured in AD (133Xe) and M (plethysmography). Results: The area under the curve divided by time (AUC0-360min/360min) in obese vs. nonobese was as follows: 1) arterial glucose was similar 6.04 ± 0.2 vs. 5.67 ± 0.1 mM), but insulin was increased (65.5 ± 6.6 vs. 28.7 ± 1.7 mU/liter, P = 0.0004); 2) BF was decreased (3 ± 0.2 vs. 4.4 ± 0.3 ml/min per 100 ml tissue in M, P = 0.002 and 1.8 ± 0.1 vs. 3.7 ± 0.3 ml/min per 100 ml tissue in AD, P < 0.0001); 3) glucose uptake was decreased (0.9 ± 0.1 vs. 2.3 ± 0.4 μmol/min per 100 ml tissue in M, P = 0.002 and 0.45 ± 0.1 vs. 1.1 ± 0.17 μmol/min per 100 ml tissue in AD, P = 0.01); 4) fractional glucose extraction was decreased in M (5 ± 1 vs. 9 ± 1%, P = 0.03), but was similar in AD (3 ± 1 vs. 3.6 ± 1.4%); 5) glucose uptake (per total fat mass) was increased (0.275 ± 0.04 vs. 0.12 ± 0.02 mmol/min, P = 0.027). Conclusion: In morbid obesity, the sensitivity of glucose metabolism to insulin is impaired in M, due to defects in insulin-stimulated glucose use and decreased BF, and in AD, at least in part, due to decreased BF. However, increased total fat mass provides a sink for the excess of glucose and compensates for insulin resistance.

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