Rates of lipid fluxes in adipose tissue in vivo after a mixed meal in morbid obesity

P. Mitrou, E. Boutati, V. Lambadiari, E. Maratou, V. Komesidou, A. Papakonstantinou, L. Sidossis, N. Tountas, N. Katsilambros, T. Economopoulos, S. A. Raptis, G. Dimitriadis

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective:Although insulin resistance in obesity is established, information on insulin action on lipid fluxes, in morbid obesity, is limited. This study was undertaken in morbidly obese women to investigate insulin action on triacylglycerol fluxes and lipolysis across adipose tissue.Subjects and Design:A meal was given to 26 obese (age 35±1years, body mass index 46±1 kg m-2) and 11 non-obese women (age 38±2years, body mass index 24±1 kg m-2). Plasma samples for glucose, insulin, triglycerides and non-esterified fatty acids (NEFAs) were taken for 360 min from a vein draining the abdominal subcutaneous adipose tissue and from the radial artery. Adipose tissue blood flow was measured with 133Xe. Results:In obese vs non-obese: (1) Arterial glucose was similar, but insulin was increased (P=0.0001). (2) Adipose tissue blood flow was decreased (P0.0001). (3) Arterial triglycerides (P=0.0001) and NEFAs (P=0.01) were increased. (4) Lipoprotein lipase was decreased (P=0.0009), although the arteriovenous triglyceride differences were similar. (5) Veno-arterial NEFA differences across the adipose tissue were similar. (6) NEFA fluxes and hormone-sensitive lipase-derived glycerol output from 100 g adipose tissue were not different. (7) Total adipose tissue NEFA release was increased (P0.02).Conclusions:In morbid obesity: (a) hypertriglycerinemia could be attributed to a defect in the postprandial dynamic adjustment of triglyceride clearance across the adipose tissue, partly caused by blunted BF; and (b) postprandially, there is an impairment of adipose tissue to buffer NEFA excess, despite hyperinsulinemia.

Original languageEnglish (US)
Pages (from-to)770-774
Number of pages5
JournalInternational Journal of Obesity
Volume34
Issue number4
DOIs
StatePublished - Apr 2010
Externally publishedYes

Fingerprint

Morbid Obesity
Meals
Adipose Tissue
Lipids
Fatty Acids
Triglycerides
Insulin
Body Mass Index
Abdominal Subcutaneous Fat
Sterol Esterase
Glucose
Radial Artery
Lipoprotein Lipase
Lipolysis
Hyperinsulinism
Glycerol
Insulin Resistance
Veins
Buffers
Obesity

Keywords

  • Adipose tissue
  • Insulin action
  • Lipid fluxes
  • Morbid obesity
  • Non-esterified fatty acids
  • Triglycerides

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Endocrinology, Diabetes and Metabolism
  • Medicine(all)

Cite this

Mitrou, P., Boutati, E., Lambadiari, V., Maratou, E., Komesidou, V., Papakonstantinou, A., ... Dimitriadis, G. (2010). Rates of lipid fluxes in adipose tissue in vivo after a mixed meal in morbid obesity. International Journal of Obesity, 34(4), 770-774. https://doi.org/10.1038/ijo.2009.293

Rates of lipid fluxes in adipose tissue in vivo after a mixed meal in morbid obesity. / Mitrou, P.; Boutati, E.; Lambadiari, V.; Maratou, E.; Komesidou, V.; Papakonstantinou, A.; Sidossis, L.; Tountas, N.; Katsilambros, N.; Economopoulos, T.; Raptis, S. A.; Dimitriadis, G.

In: International Journal of Obesity, Vol. 34, No. 4, 04.2010, p. 770-774.

Research output: Contribution to journalArticle

Mitrou, P, Boutati, E, Lambadiari, V, Maratou, E, Komesidou, V, Papakonstantinou, A, Sidossis, L, Tountas, N, Katsilambros, N, Economopoulos, T, Raptis, SA & Dimitriadis, G 2010, 'Rates of lipid fluxes in adipose tissue in vivo after a mixed meal in morbid obesity', International Journal of Obesity, vol. 34, no. 4, pp. 770-774. https://doi.org/10.1038/ijo.2009.293
Mitrou P, Boutati E, Lambadiari V, Maratou E, Komesidou V, Papakonstantinou A et al. Rates of lipid fluxes in adipose tissue in vivo after a mixed meal in morbid obesity. International Journal of Obesity. 2010 Apr;34(4):770-774. https://doi.org/10.1038/ijo.2009.293
Mitrou, P. ; Boutati, E. ; Lambadiari, V. ; Maratou, E. ; Komesidou, V. ; Papakonstantinou, A. ; Sidossis, L. ; Tountas, N. ; Katsilambros, N. ; Economopoulos, T. ; Raptis, S. A. ; Dimitriadis, G. / Rates of lipid fluxes in adipose tissue in vivo after a mixed meal in morbid obesity. In: International Journal of Obesity. 2010 ; Vol. 34, No. 4. pp. 770-774.
@article{f21191deaf824b558877e4a031713629,
title = "Rates of lipid fluxes in adipose tissue in vivo after a mixed meal in morbid obesity",
abstract = "Objective:Although insulin resistance in obesity is established, information on insulin action on lipid fluxes, in morbid obesity, is limited. This study was undertaken in morbidly obese women to investigate insulin action on triacylglycerol fluxes and lipolysis across adipose tissue.Subjects and Design:A meal was given to 26 obese (age 35±1years, body mass index 46±1 kg m-2) and 11 non-obese women (age 38±2years, body mass index 24±1 kg m-2). Plasma samples for glucose, insulin, triglycerides and non-esterified fatty acids (NEFAs) were taken for 360 min from a vein draining the abdominal subcutaneous adipose tissue and from the radial artery. Adipose tissue blood flow was measured with 133Xe. Results:In obese vs non-obese: (1) Arterial glucose was similar, but insulin was increased (P=0.0001). (2) Adipose tissue blood flow was decreased (P0.0001). (3) Arterial triglycerides (P=0.0001) and NEFAs (P=0.01) were increased. (4) Lipoprotein lipase was decreased (P=0.0009), although the arteriovenous triglyceride differences were similar. (5) Veno-arterial NEFA differences across the adipose tissue were similar. (6) NEFA fluxes and hormone-sensitive lipase-derived glycerol output from 100 g adipose tissue were not different. (7) Total adipose tissue NEFA release was increased (P0.02).Conclusions:In morbid obesity: (a) hypertriglycerinemia could be attributed to a defect in the postprandial dynamic adjustment of triglyceride clearance across the adipose tissue, partly caused by blunted BF; and (b) postprandially, there is an impairment of adipose tissue to buffer NEFA excess, despite hyperinsulinemia.",
keywords = "Adipose tissue, Insulin action, Lipid fluxes, Morbid obesity, Non-esterified fatty acids, Triglycerides",
author = "P. Mitrou and E. Boutati and V. Lambadiari and E. Maratou and V. Komesidou and A. Papakonstantinou and L. Sidossis and N. Tountas and N. Katsilambros and T. Economopoulos and Raptis, {S. A.} and G. Dimitriadis",
year = "2010",
month = "4",
doi = "10.1038/ijo.2009.293",
language = "English (US)",
volume = "34",
pages = "770--774",
journal = "International Journal of Obesity",
issn = "0307-0565",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - Rates of lipid fluxes in adipose tissue in vivo after a mixed meal in morbid obesity

AU - Mitrou, P.

AU - Boutati, E.

AU - Lambadiari, V.

AU - Maratou, E.

AU - Komesidou, V.

AU - Papakonstantinou, A.

AU - Sidossis, L.

AU - Tountas, N.

AU - Katsilambros, N.

AU - Economopoulos, T.

AU - Raptis, S. A.

AU - Dimitriadis, G.

PY - 2010/4

Y1 - 2010/4

N2 - Objective:Although insulin resistance in obesity is established, information on insulin action on lipid fluxes, in morbid obesity, is limited. This study was undertaken in morbidly obese women to investigate insulin action on triacylglycerol fluxes and lipolysis across adipose tissue.Subjects and Design:A meal was given to 26 obese (age 35±1years, body mass index 46±1 kg m-2) and 11 non-obese women (age 38±2years, body mass index 24±1 kg m-2). Plasma samples for glucose, insulin, triglycerides and non-esterified fatty acids (NEFAs) were taken for 360 min from a vein draining the abdominal subcutaneous adipose tissue and from the radial artery. Adipose tissue blood flow was measured with 133Xe. Results:In obese vs non-obese: (1) Arterial glucose was similar, but insulin was increased (P=0.0001). (2) Adipose tissue blood flow was decreased (P0.0001). (3) Arterial triglycerides (P=0.0001) and NEFAs (P=0.01) were increased. (4) Lipoprotein lipase was decreased (P=0.0009), although the arteriovenous triglyceride differences were similar. (5) Veno-arterial NEFA differences across the adipose tissue were similar. (6) NEFA fluxes and hormone-sensitive lipase-derived glycerol output from 100 g adipose tissue were not different. (7) Total adipose tissue NEFA release was increased (P0.02).Conclusions:In morbid obesity: (a) hypertriglycerinemia could be attributed to a defect in the postprandial dynamic adjustment of triglyceride clearance across the adipose tissue, partly caused by blunted BF; and (b) postprandially, there is an impairment of adipose tissue to buffer NEFA excess, despite hyperinsulinemia.

AB - Objective:Although insulin resistance in obesity is established, information on insulin action on lipid fluxes, in morbid obesity, is limited. This study was undertaken in morbidly obese women to investigate insulin action on triacylglycerol fluxes and lipolysis across adipose tissue.Subjects and Design:A meal was given to 26 obese (age 35±1years, body mass index 46±1 kg m-2) and 11 non-obese women (age 38±2years, body mass index 24±1 kg m-2). Plasma samples for glucose, insulin, triglycerides and non-esterified fatty acids (NEFAs) were taken for 360 min from a vein draining the abdominal subcutaneous adipose tissue and from the radial artery. Adipose tissue blood flow was measured with 133Xe. Results:In obese vs non-obese: (1) Arterial glucose was similar, but insulin was increased (P=0.0001). (2) Adipose tissue blood flow was decreased (P0.0001). (3) Arterial triglycerides (P=0.0001) and NEFAs (P=0.01) were increased. (4) Lipoprotein lipase was decreased (P=0.0009), although the arteriovenous triglyceride differences were similar. (5) Veno-arterial NEFA differences across the adipose tissue were similar. (6) NEFA fluxes and hormone-sensitive lipase-derived glycerol output from 100 g adipose tissue were not different. (7) Total adipose tissue NEFA release was increased (P0.02).Conclusions:In morbid obesity: (a) hypertriglycerinemia could be attributed to a defect in the postprandial dynamic adjustment of triglyceride clearance across the adipose tissue, partly caused by blunted BF; and (b) postprandially, there is an impairment of adipose tissue to buffer NEFA excess, despite hyperinsulinemia.

KW - Adipose tissue

KW - Insulin action

KW - Lipid fluxes

KW - Morbid obesity

KW - Non-esterified fatty acids

KW - Triglycerides

UR - http://www.scopus.com/inward/record.url?scp=77950858681&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950858681&partnerID=8YFLogxK

U2 - 10.1038/ijo.2009.293

DO - 10.1038/ijo.2009.293

M3 - Article

VL - 34

SP - 770

EP - 774

JO - International Journal of Obesity

JF - International Journal of Obesity

SN - 0307-0565

IS - 4

ER -