Reactive oxygen species affect spinal cell type-specific synaptic plasticity in a model of neuropathic pain

Alice Bittar, Jaebeom Jun, Jun-Ho La, Jigong Wang, Joong Woo Leem, Jin Chung

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Spinal synaptic plasticity is believed to drive central sensitization that underlies the persistent nature of neuropathic pain. Our recent data showed that synaptic plasticity in the dorsal horn is cell type specific: intense afferent stimulation produced long-term potentiation (LTP) in excitatory spinothalamic tract neurons (STTn), whereas it produced long-term depression (LTD) in inhibitory GABAergic interneurons (GABAn). In addition, reactive oxygen species (ROS) were shown to be involved in LTP in STTn (STTn-LTP) and in LTD in GABAn (GABAn-LTD). This study examined the roles of 2 biologically important ROS - superoxide [·O 2 ] and hydroxyl radicals [·OH] - in neuropathic mechanical hyperalgesia and cell type-specific spinal synaptic plasticity. The [·O 2 ] donor induced stronger mechanical hyperalgesia than the [·OH] donor in naive mice. The [·O 2 ] scavenger showed greater antihyperalgesic effect than [·OH] scavengers in the spinal nerve ligation (SNL) mouse model of neuropathic pain. In addition, the [·O 2 ] donor induced both STTn-LTP and GABAn-LTD, but the [·OH] donor induced only GABAn-LTD. On the other hand, the [·O 2 ] scavenger inhibited STTn-LTP and GABAn-LTD induction in naive mice and alleviated SNL-induced potentiation in STTn and depression in GABAn. The [·OH] scavenger, however, inhibited depression in GABAn but did not interfere with potentiation in STTn. These results indicate that mechanical hyperalgesia in SNL mice is the result of the combination of STTn-LTP and GABAn-LTD. Behavioral outcomes compliment electrophysiological results which suggest that [·O 2 ] mediates both STTn-LTP and GABAn-LTD, whereas [·OH] is involved primarily in GABAn-LTD.

Original languageEnglish (US)
Pages (from-to)2137-2146
Number of pages10
JournalPain
Volume158
Issue number11
DOIs
StatePublished - Nov 1 2017

Fingerprint

Spinothalamic Tracts
Neuronal Plasticity
Neuralgia
Interneurons
Reactive Oxygen Species
Long-Term Potentiation
Neurons
Spinal Nerves
Hyperalgesia
Ligation
Central Nervous System Sensitization
Posterior Horn Cells
Superoxides
Hydroxyl Radical

Keywords

  • GABAergic interneurons
  • Long term depression
  • Long term potentiation
  • Neuropathic pain
  • Pain behavior
  • Reactive oxygen species
  • Spinal cord
  • Spinal nerve ligation
  • Spinothalamic tract neurons
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Anesthesiology and Pain Medicine

Cite this

Reactive oxygen species affect spinal cell type-specific synaptic plasticity in a model of neuropathic pain. / Bittar, Alice; Jun, Jaebeom; La, Jun-Ho; Wang, Jigong; Leem, Joong Woo; Chung, Jin.

In: Pain, Vol. 158, No. 11, 01.11.2017, p. 2137-2146.

Research output: Contribution to journalArticle

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abstract = "Spinal synaptic plasticity is believed to drive central sensitization that underlies the persistent nature of neuropathic pain. Our recent data showed that synaptic plasticity in the dorsal horn is cell type specific: intense afferent stimulation produced long-term potentiation (LTP) in excitatory spinothalamic tract neurons (STTn), whereas it produced long-term depression (LTD) in inhibitory GABAergic interneurons (GABAn). In addition, reactive oxygen species (ROS) were shown to be involved in LTP in STTn (STTn-LTP) and in LTD in GABAn (GABAn-LTD). This study examined the roles of 2 biologically important ROS - superoxide [·O 2 ] and hydroxyl radicals [·OH] - in neuropathic mechanical hyperalgesia and cell type-specific spinal synaptic plasticity. The [·O 2 ] donor induced stronger mechanical hyperalgesia than the [·OH] donor in naive mice. The [·O 2 ] scavenger showed greater antihyperalgesic effect than [·OH] scavengers in the spinal nerve ligation (SNL) mouse model of neuropathic pain. In addition, the [·O 2 ] donor induced both STTn-LTP and GABAn-LTD, but the [·OH] donor induced only GABAn-LTD. On the other hand, the [·O 2 ] scavenger inhibited STTn-LTP and GABAn-LTD induction in naive mice and alleviated SNL-induced potentiation in STTn and depression in GABAn. The [·OH] scavenger, however, inhibited depression in GABAn but did not interfere with potentiation in STTn. These results indicate that mechanical hyperalgesia in SNL mice is the result of the combination of STTn-LTP and GABAn-LTD. Behavioral outcomes compliment electrophysiological results which suggest that [·O 2 ] mediates both STTn-LTP and GABAn-LTD, whereas [·OH] is involved primarily in GABAn-LTD.",
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AU - Jun, Jaebeom

AU - La, Jun-Ho

AU - Wang, Jigong

AU - Leem, Joong Woo

AU - Chung, Jin

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N2 - Spinal synaptic plasticity is believed to drive central sensitization that underlies the persistent nature of neuropathic pain. Our recent data showed that synaptic plasticity in the dorsal horn is cell type specific: intense afferent stimulation produced long-term potentiation (LTP) in excitatory spinothalamic tract neurons (STTn), whereas it produced long-term depression (LTD) in inhibitory GABAergic interneurons (GABAn). In addition, reactive oxygen species (ROS) were shown to be involved in LTP in STTn (STTn-LTP) and in LTD in GABAn (GABAn-LTD). This study examined the roles of 2 biologically important ROS - superoxide [·O 2 ] and hydroxyl radicals [·OH] - in neuropathic mechanical hyperalgesia and cell type-specific spinal synaptic plasticity. The [·O 2 ] donor induced stronger mechanical hyperalgesia than the [·OH] donor in naive mice. The [·O 2 ] scavenger showed greater antihyperalgesic effect than [·OH] scavengers in the spinal nerve ligation (SNL) mouse model of neuropathic pain. In addition, the [·O 2 ] donor induced both STTn-LTP and GABAn-LTD, but the [·OH] donor induced only GABAn-LTD. On the other hand, the [·O 2 ] scavenger inhibited STTn-LTP and GABAn-LTD induction in naive mice and alleviated SNL-induced potentiation in STTn and depression in GABAn. The [·OH] scavenger, however, inhibited depression in GABAn but did not interfere with potentiation in STTn. These results indicate that mechanical hyperalgesia in SNL mice is the result of the combination of STTn-LTP and GABAn-LTD. Behavioral outcomes compliment electrophysiological results which suggest that [·O 2 ] mediates both STTn-LTP and GABAn-LTD, whereas [·OH] is involved primarily in GABAn-LTD.

AB - Spinal synaptic plasticity is believed to drive central sensitization that underlies the persistent nature of neuropathic pain. Our recent data showed that synaptic plasticity in the dorsal horn is cell type specific: intense afferent stimulation produced long-term potentiation (LTP) in excitatory spinothalamic tract neurons (STTn), whereas it produced long-term depression (LTD) in inhibitory GABAergic interneurons (GABAn). In addition, reactive oxygen species (ROS) were shown to be involved in LTP in STTn (STTn-LTP) and in LTD in GABAn (GABAn-LTD). This study examined the roles of 2 biologically important ROS - superoxide [·O 2 ] and hydroxyl radicals [·OH] - in neuropathic mechanical hyperalgesia and cell type-specific spinal synaptic plasticity. The [·O 2 ] donor induced stronger mechanical hyperalgesia than the [·OH] donor in naive mice. The [·O 2 ] scavenger showed greater antihyperalgesic effect than [·OH] scavengers in the spinal nerve ligation (SNL) mouse model of neuropathic pain. In addition, the [·O 2 ] donor induced both STTn-LTP and GABAn-LTD, but the [·OH] donor induced only GABAn-LTD. On the other hand, the [·O 2 ] scavenger inhibited STTn-LTP and GABAn-LTD induction in naive mice and alleviated SNL-induced potentiation in STTn and depression in GABAn. The [·OH] scavenger, however, inhibited depression in GABAn but did not interfere with potentiation in STTn. These results indicate that mechanical hyperalgesia in SNL mice is the result of the combination of STTn-LTP and GABAn-LTD. Behavioral outcomes compliment electrophysiological results which suggest that [·O 2 ] mediates both STTn-LTP and GABAn-LTD, whereas [·OH] is involved primarily in GABAn-LTD.

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