Reactive oxygen species (ROS) play an important role in a rat model of neuropathic pain

Hee Kee Kim, Soon Kwon Park, Jun Li Zhou, Giulio Taglialatela, Kyungsoon Chung, Richard E. Coggeshall, Jin Chung

Research output: Contribution to journalArticle

289 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) are free radicals produced in biological systems that are involved in various degenerative brain diseases. The present study tests the hypothesis that ROS also play an important role in neuropathic pain. In the rat spinal nerve ligation (SNL) model of neuropathic pain, mechanical allodynia develops fully 3 days after nerve ligation and persists for many weeks. Systemic injection of a ROS scavenger, phenyl-N-tert-butylnitrone (PBN), relieves SNL-induced mechanical allodynia in a dose-dependent manner. Repeated injections cause no development of tolerance or no loss of potency. Preemptive treatment with PBN is also effective in preventing full development of neuropathic pain behavior. Systemic injection was mimicked by intrathecal injection with a little less efficacy, while intracerebroventricular administration produced a much smaller effect. These data suggest that PBN exerts its anti-allodynic action mainly by spinal mechanisms. Systemic treatment with other spin-trap reagents, 5,5-dimethylpyrroline-N-oxide and nitrosobenzene, showed similar analgesic effects, suggesting that ROS are critically involved in the development and maintenance of neuropathic pain. Thus this study suggests that systemic administration of non-toxic doses of free radical scavengers could be useful for treatment of neuropathic pain.

Original languageEnglish (US)
Pages (from-to)116-124
Number of pages9
JournalPain
Volume111
Issue number1-2
DOIs
StatePublished - Sep 2004

Fingerprint

Neuralgia
Reactive Oxygen Species
Ligation
Spinal Nerves
Hyperalgesia
Injections
Spinal Injections
Free Radical Scavengers
Brain Diseases
Oxides
Free Radicals
Analgesics
Therapeutics
Maintenance
phenyl-N-tert-butylnitrone

Keywords

  • Anti-allodynia
  • Free radicals
  • PBN
  • Spinal nerve ligation model

ASJC Scopus subject areas

  • Clinical Neurology
  • Psychiatry and Mental health
  • Neurology
  • Neuroscience(all)
  • Pharmacology
  • Clinical Psychology

Cite this

Reactive oxygen species (ROS) play an important role in a rat model of neuropathic pain. / Kim, Hee Kee; Park, Soon Kwon; Zhou, Jun Li; Taglialatela, Giulio; Chung, Kyungsoon; Coggeshall, Richard E.; Chung, Jin.

In: Pain, Vol. 111, No. 1-2, 09.2004, p. 116-124.

Research output: Contribution to journalArticle

Kim, Hee Kee ; Park, Soon Kwon ; Zhou, Jun Li ; Taglialatela, Giulio ; Chung, Kyungsoon ; Coggeshall, Richard E. ; Chung, Jin. / Reactive oxygen species (ROS) play an important role in a rat model of neuropathic pain. In: Pain. 2004 ; Vol. 111, No. 1-2. pp. 116-124.
@article{4e92047a109e479687f6965ad53ae831,
title = "Reactive oxygen species (ROS) play an important role in a rat model of neuropathic pain",
abstract = "Reactive oxygen species (ROS) are free radicals produced in biological systems that are involved in various degenerative brain diseases. The present study tests the hypothesis that ROS also play an important role in neuropathic pain. In the rat spinal nerve ligation (SNL) model of neuropathic pain, mechanical allodynia develops fully 3 days after nerve ligation and persists for many weeks. Systemic injection of a ROS scavenger, phenyl-N-tert-butylnitrone (PBN), relieves SNL-induced mechanical allodynia in a dose-dependent manner. Repeated injections cause no development of tolerance or no loss of potency. Preemptive treatment with PBN is also effective in preventing full development of neuropathic pain behavior. Systemic injection was mimicked by intrathecal injection with a little less efficacy, while intracerebroventricular administration produced a much smaller effect. These data suggest that PBN exerts its anti-allodynic action mainly by spinal mechanisms. Systemic treatment with other spin-trap reagents, 5,5-dimethylpyrroline-N-oxide and nitrosobenzene, showed similar analgesic effects, suggesting that ROS are critically involved in the development and maintenance of neuropathic pain. Thus this study suggests that systemic administration of non-toxic doses of free radical scavengers could be useful for treatment of neuropathic pain.",
keywords = "Anti-allodynia, Free radicals, PBN, Spinal nerve ligation model",
author = "Kim, {Hee Kee} and Park, {Soon Kwon} and Zhou, {Jun Li} and Giulio Taglialatela and Kyungsoon Chung and Coggeshall, {Richard E.} and Jin Chung",
year = "2004",
month = "9",
doi = "10.1016/j.pain.2004.06.008",
language = "English (US)",
volume = "111",
pages = "116--124",
journal = "Pain",
issn = "0304-3959",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Reactive oxygen species (ROS) play an important role in a rat model of neuropathic pain

AU - Kim, Hee Kee

AU - Park, Soon Kwon

AU - Zhou, Jun Li

AU - Taglialatela, Giulio

AU - Chung, Kyungsoon

AU - Coggeshall, Richard E.

AU - Chung, Jin

PY - 2004/9

Y1 - 2004/9

N2 - Reactive oxygen species (ROS) are free radicals produced in biological systems that are involved in various degenerative brain diseases. The present study tests the hypothesis that ROS also play an important role in neuropathic pain. In the rat spinal nerve ligation (SNL) model of neuropathic pain, mechanical allodynia develops fully 3 days after nerve ligation and persists for many weeks. Systemic injection of a ROS scavenger, phenyl-N-tert-butylnitrone (PBN), relieves SNL-induced mechanical allodynia in a dose-dependent manner. Repeated injections cause no development of tolerance or no loss of potency. Preemptive treatment with PBN is also effective in preventing full development of neuropathic pain behavior. Systemic injection was mimicked by intrathecal injection with a little less efficacy, while intracerebroventricular administration produced a much smaller effect. These data suggest that PBN exerts its anti-allodynic action mainly by spinal mechanisms. Systemic treatment with other spin-trap reagents, 5,5-dimethylpyrroline-N-oxide and nitrosobenzene, showed similar analgesic effects, suggesting that ROS are critically involved in the development and maintenance of neuropathic pain. Thus this study suggests that systemic administration of non-toxic doses of free radical scavengers could be useful for treatment of neuropathic pain.

AB - Reactive oxygen species (ROS) are free radicals produced in biological systems that are involved in various degenerative brain diseases. The present study tests the hypothesis that ROS also play an important role in neuropathic pain. In the rat spinal nerve ligation (SNL) model of neuropathic pain, mechanical allodynia develops fully 3 days after nerve ligation and persists for many weeks. Systemic injection of a ROS scavenger, phenyl-N-tert-butylnitrone (PBN), relieves SNL-induced mechanical allodynia in a dose-dependent manner. Repeated injections cause no development of tolerance or no loss of potency. Preemptive treatment with PBN is also effective in preventing full development of neuropathic pain behavior. Systemic injection was mimicked by intrathecal injection with a little less efficacy, while intracerebroventricular administration produced a much smaller effect. These data suggest that PBN exerts its anti-allodynic action mainly by spinal mechanisms. Systemic treatment with other spin-trap reagents, 5,5-dimethylpyrroline-N-oxide and nitrosobenzene, showed similar analgesic effects, suggesting that ROS are critically involved in the development and maintenance of neuropathic pain. Thus this study suggests that systemic administration of non-toxic doses of free radical scavengers could be useful for treatment of neuropathic pain.

KW - Anti-allodynia

KW - Free radicals

KW - PBN

KW - Spinal nerve ligation model

UR - http://www.scopus.com/inward/record.url?scp=4344589996&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4344589996&partnerID=8YFLogxK

U2 - 10.1016/j.pain.2004.06.008

DO - 10.1016/j.pain.2004.06.008

M3 - Article

VL - 111

SP - 116

EP - 124

JO - Pain

JF - Pain

SN - 0304-3959

IS - 1-2

ER -