Real-time kinetics of high-mobility group box 1 (HMGB1) oxidation in extracellular fluids studied by in situ protein NMR spectroscopy

Levani Zandarashvili, Debashish Sahu, Kwanbok Lee, Yong Sun Lee, Pomila Singh, Krishna Rajarathnam, Junji Iwahara

Research output: Contribution to journalArticle

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Abstract

Some extracellular proteins are initially secreted in reduced forms via a non-canonical pathway bypassing the endoplasmic reticulum and become oxidized in the extracellular space. One such protein is HMGB1 (high-mobility group box 1). Extracellular HMGB1 has different redox states that play distinct roles in inflammation. Using a unique NMR-based approach, we have investigated the kinetics of HMGB1 oxidation and the half-lives of all-thiol and disulfide HMGB1 species in serum, saliva, and cell culture medium. In this approach, salt-free lyophilized 15N- labeled all-thiol HMGB1 was dissolved in actual extracellular fluids, and the oxidation and clearance kinetics were monitored in situ by recording a series of heteronuclear 1H-15N correlation spectra. We found that the half-life depends significantly on the extracellular environment. For example, the half-life of all-thiol HMGB1 ranged from ∼17 min (in human serum and saliva) to 3 h (in prostate cancer cell culture medium). Furthermore, the binding of ligands (glycyrrhizin and heparin) to HMGB1 significantly modulated the oxidation kinetics. Thus, the balance between the roles of all-thiol and disulfide HMGB1 proteins depends significantly on the extracellular environment and can also be artificially modulated by ligands. This is important because extracellular HMGB1 has been suggested as a therapeutic target for inflammatory diseases and cancer. Our work demonstrates that the in situ protein NMR approach is powerful for investigating the behavior of proteins in actual extracellular fluids containing an enormous number of different molecules.

Original languageEnglish (US)
Pages (from-to)11621-11627
Number of pages7
JournalJournal of Biological Chemistry
Volume288
Issue number17
DOIs
StatePublished - Apr 26 2013

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Biomolecular Nuclear Magnetic Resonance
Extracellular Fluid
Sulfhydryl Compounds
Nuclear magnetic resonance spectroscopy
HMGB1 Protein
Oxidation
Kinetics
Fluids
Saliva
Cell culture
Disulfides
Half-Life
Culture Media
Proteins
Cell Culture Techniques
Nuclear magnetic resonance
Glycyrrhizic Acid
Ligands
Extracellular Space
Serum

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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Real-time kinetics of high-mobility group box 1 (HMGB1) oxidation in extracellular fluids studied by in situ protein NMR spectroscopy. / Zandarashvili, Levani; Sahu, Debashish; Lee, Kwanbok; Lee, Yong Sun; Singh, Pomila; Rajarathnam, Krishna; Iwahara, Junji.

In: Journal of Biological Chemistry, Vol. 288, No. 17, 26.04.2013, p. 11621-11627.

Research output: Contribution to journalArticle

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