Real-time PCR assay targeting IS481 of bordetella pertussis and molecular basis for detecting bordetella holmesii

U. Reischl, N. Lehn, G. N. Sanden, M. J. Loeffelholz

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Detection of Bordetella holmesii by a real-time PCR assay targeting IS481 of Bordetella pertussis is reported. Sequencing of IS481-specific PCR products from B. pertussis and B. holmesii isolates revealed sequence homology. Restriction fragment length polymorphism demonstrated a low copy number of IS481-like sequences in B. holmesii. These results, and culture of B. holmesii from patients with cough, suggest that the specificity and predictive value of IS481-based PCR assays for pertussis may be compromised.

Original languageEnglish (US)
Pages (from-to)1963-1966
Number of pages4
JournalJournal of Clinical Microbiology
Volume39
Issue number5
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Bordetella
Bordetella pertussis
Real-Time Polymerase Chain Reaction
Polymerase Chain Reaction
Whooping Cough
Sequence Homology
Cough
Restriction Fragment Length Polymorphisms

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Real-time PCR assay targeting IS481 of bordetella pertussis and molecular basis for detecting bordetella holmesii. / Reischl, U.; Lehn, N.; Sanden, G. N.; Loeffelholz, M. J.

In: Journal of Clinical Microbiology, Vol. 39, No. 5, 2001, p. 1963-1966.

Research output: Contribution to journalArticle

Reischl, U. ; Lehn, N. ; Sanden, G. N. ; Loeffelholz, M. J. / Real-time PCR assay targeting IS481 of bordetella pertussis and molecular basis for detecting bordetella holmesii. In: Journal of Clinical Microbiology. 2001 ; Vol. 39, No. 5. pp. 1963-1966.
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