Real-time PCR assay targeting IS481 of bordetella pertussis and molecular basis for detecting bordetella holmesii

U. Reischl, N. Lehn, G. N. Sanden, M. J. Loeffelholz

Research output: Contribution to journalArticle

123 Scopus citations

Abstract

Detection of Bordetella holmesii by a real-time PCR assay targeting IS481 of Bordetella pertussis is reported. Sequencing of IS481-specific PCR products from B. pertussis and B. holmesii isolates revealed sequence homology. Restriction fragment length polymorphism demonstrated a low copy number of IS481-like sequences in B. holmesii. These results, and culture of B. holmesii from patients with cough, suggest that the specificity and predictive value of IS481-based PCR assays for pertussis may be compromised.

Original languageEnglish (US)
Pages (from-to)1963-1966
Number of pages4
JournalJournal of Clinical Microbiology
Volume39
Issue number5
DOIs
StatePublished - 2001
Externally publishedYes

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ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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