Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Shannon L. Emery, Dean D. Erdman, Michael D. Bowen, Bruce R. Newton, Jonas M. Winchell, Richard F. Meyer, Suxiang Tong, Byron T. Cook, Brian P. Holloway, Karen A. McCaustland, Paul A. Rota, Bettina Bankamp, Luis E. Lowe, Thomas Ksiazek, William J. Bellini, Larry J. Anderson

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.

Original languageEnglish (US)
Pages (from-to)311-316
Number of pages6
JournalEmerging Infectious Diseases
Volume10
Issue number2
StatePublished - Feb 2004
Externally publishedYes

Fingerprint

SARS Virus
Severe Acute Respiratory Syndrome
Coronavirus
Reverse Transcription
Polymerase Chain Reaction
Coronavirus Infections
Limit of Detection
Genome

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Emery, S. L., Erdman, D. D., Bowen, M. D., Newton, B. R., Winchell, J. M., Meyer, R. F., ... Anderson, L. J. (2004). Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus. Emerging Infectious Diseases, 10(2), 311-316.

Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus. / Emery, Shannon L.; Erdman, Dean D.; Bowen, Michael D.; Newton, Bruce R.; Winchell, Jonas M.; Meyer, Richard F.; Tong, Suxiang; Cook, Byron T.; Holloway, Brian P.; McCaustland, Karen A.; Rota, Paul A.; Bankamp, Bettina; Lowe, Luis E.; Ksiazek, Thomas; Bellini, William J.; Anderson, Larry J.

In: Emerging Infectious Diseases, Vol. 10, No. 2, 02.2004, p. 311-316.

Research output: Contribution to journalArticle

Emery, SL, Erdman, DD, Bowen, MD, Newton, BR, Winchell, JM, Meyer, RF, Tong, S, Cook, BT, Holloway, BP, McCaustland, KA, Rota, PA, Bankamp, B, Lowe, LE, Ksiazek, T, Bellini, WJ & Anderson, LJ 2004, 'Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus', Emerging Infectious Diseases, vol. 10, no. 2, pp. 311-316.
Emery SL, Erdman DD, Bowen MD, Newton BR, Winchell JM, Meyer RF et al. Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus. Emerging Infectious Diseases. 2004 Feb;10(2):311-316.
Emery, Shannon L. ; Erdman, Dean D. ; Bowen, Michael D. ; Newton, Bruce R. ; Winchell, Jonas M. ; Meyer, Richard F. ; Tong, Suxiang ; Cook, Byron T. ; Holloway, Brian P. ; McCaustland, Karen A. ; Rota, Paul A. ; Bankamp, Bettina ; Lowe, Luis E. ; Ksiazek, Thomas ; Bellini, William J. ; Anderson, Larry J. / Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus. In: Emerging Infectious Diseases. 2004 ; Vol. 10, No. 2. pp. 311-316.
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