Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Shannon L. Emery, Dean D. Erdman, Michael D. Bowen, Bruce R. Newton, Jonas M. Winchell, Richard F. Meyer, Suxiang Tong, Byron T. Cook, Brian P. Holloway, Karen A. McCaustland, Paul A. Rota, Bettina Bankamp, Luis E. Lowe, Tom G. Ksiazek, William J. Bellini, Larry J. Anderson

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.

Original languageEnglish (US)
Pages (from-to)311-316
Number of pages6
JournalEmerging infectious diseases
Volume10
Issue number2
DOIs
StatePublished - Feb 2004
Externally publishedYes

ASJC Scopus subject areas

  • Epidemiology
  • Microbiology (medical)
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus'. Together they form a unique fingerprint.

  • Cite this

    Emery, S. L., Erdman, D. D., Bowen, M. D., Newton, B. R., Winchell, J. M., Meyer, R. F., Tong, S., Cook, B. T., Holloway, B. P., McCaustland, K. A., Rota, P. A., Bankamp, B., Lowe, L. E., Ksiazek, T. G., Bellini, W. J., & Anderson, L. J. (2004). Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus. Emerging infectious diseases, 10(2), 311-316. https://doi.org/10.3201/eid1002.030759