Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Shannon L. Emery; Dean D. Erdman; Michael D. Bowen; Bruce R. Newton; Jonas M. Winchell; Richard F. Meyer; Suxiang Tong; Byron T. Cook; Brian P. Holloway; Karen A. McCaustland; Paul A. Rota; Bettina Bankamp; Luis E. Lowe; Tom G. Ksiazek; William J. Bellini; Larry J. Anderson

doi:10.3201/eid1002.030759

Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Shannon L. Emery
, Dean D. Erdman
, Michael D. Bowen
, Bruce R. Newton
, Jonas M. Winchell
, Richard F. Meyer
, Suxiang Tong
, Byron T. Cook
, Brian P. Holloway
, Karen A. McCaustland
, Paul A. Rota
, Bettina Bankamp
, Luis E. Lowe
, Tom G. Ksiazek
, William J. Bellini
, Larry J. Anderson

Research output: Contribution to journal › Article › peer-review

296 Scopus citations

Abstract

A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.

Original language	English (US)
Pages (from-to)	311-316
Number of pages	6
Journal	Emerging infectious diseases
Volume	10
Issue number	2
DOIs	https://doi.org/10.3201/eid1002.030759
State	Published - Feb 2004
Externally published	Yes

ASJC Scopus subject areas

Epidemiology
Microbiology (medical)
Infectious Diseases

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Emery, S. L., Erdman, D. D., Bowen, M. D., Newton, B. R., Winchell, J. M., Meyer, R. F., Tong, S., Cook, B. T., Holloway, B. P., McCaustland, K. A., Rota, P. A., Bankamp, B., Lowe, L. E., Ksiazek, T. G., Bellini, W. J., & Anderson, L. J. (2004). Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus. Emerging infectious diseases, 10(2), 311-316. https://doi.org/10.3201/eid1002.030759

Emery, SL, Erdman, DD, Bowen, MD, Newton, BR, Winchell, JM, Meyer, RF, Tong, S, Cook, BT, Holloway, BP, McCaustland, KA, Rota, PA, Bankamp, B, Lowe, LE, Ksiazek, TG, Bellini, WJ & Anderson, LJ 2004, 'Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus', Emerging infectious diseases, vol. 10, no. 2, pp. 311-316. https://doi.org/10.3201/eid1002.030759

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abstract = "A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.",

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Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Abstract

ASJC Scopus subject areas

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