Rebound contraction by nitric oxide in the longitudinal muscle of porcine gastric fundus

Jun-Ho La, Taewan Kim, Taesik Sung, Jeongwoo Kang, Hyunju Song, Hyunju Kim, Ilsuk Yang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The rebound contraction induced by electrical field stimulation (EFS) and nitric oxide (NO) donor, S-nitroso-L-cysteine (cysNO), were investigated in the longitudinal muscle of porcine gastric fundus (LM-PGF). Under the presence of atropine and guanethidine, cysNO and EFS produced sequential relaxation-contraction in LM-PGF. Tetrodotoxin abolished the EFS-induced response, while leaving the cysNO-induced one unaffected. A soluble guanylate cyclase inhibitor, 1H-[1,2,4]-oxadiazolo-[4,3-a]-quinoxalin-1-one, inhibited both cysNO and EFS-induced biphasic response. A cGMP analogue only relaxed LM-PGF. A phosphodiesterase V inhibitor, zaprinast, prolonged the cysNO and the EFS-induced relaxation and inhibited the rebound contraction. The rebound contraction was inhibited by verapamil, an L-type Ca2+ channel blocker. The cysNO and the EFS-induced biphasic response were inhibited by ryanodine plus cyclopiazonic acid or by ruthenium red, a ryanodine-receptor blocker. LM-PGF was relaxed on exposure to caffeine and then produced a verapamil-sensitive rebound contraction during the washout period. CysNO and EFS did not induce the rebound contraction in the presence of caffeine. These results suggest that the NO-induced rebound contraction involves both Ca2+-release from the ryanodine-sensitive store and Ca2+-influx through L-type channels. Although the NO-induced biphasic response is dependent on cGMP, rapid removal of cGMP seems necessary for the rebound contraction.

Original languageEnglish (US)
Pages (from-to)395-404
Number of pages10
JournalJapanese Journal of Pharmacology
Volume89
Issue number4
DOIs
StatePublished - Aug 2002
Externally publishedYes

Fingerprint

Gastric Fundus
Electric Stimulation
Nitric Oxide
Swine
Muscles
Ryanodine
Verapamil
Caffeine
Type 5 Cyclic Nucleotide Phosphodiesterases
Guanethidine
Ruthenium Red
Ryanodine Receptor Calcium Release Channel
Phosphodiesterase Inhibitors
Nitric Oxide Donors
Tetrodotoxin
Atropine

Keywords

  • L-type Ca channel
  • Longitudinal muscle of porcine gastric fundus
  • Ryanodine-sensitive Ca channel
  • S-Nitroso-L-cysteine
  • Soluble guanylate cyclase

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

Cite this

Rebound contraction by nitric oxide in the longitudinal muscle of porcine gastric fundus. / La, Jun-Ho; Kim, Taewan; Sung, Taesik; Kang, Jeongwoo; Song, Hyunju; Kim, Hyunju; Yang, Ilsuk.

In: Japanese Journal of Pharmacology, Vol. 89, No. 4, 08.2002, p. 395-404.

Research output: Contribution to journalArticle

La, Jun-Ho ; Kim, Taewan ; Sung, Taesik ; Kang, Jeongwoo ; Song, Hyunju ; Kim, Hyunju ; Yang, Ilsuk. / Rebound contraction by nitric oxide in the longitudinal muscle of porcine gastric fundus. In: Japanese Journal of Pharmacology. 2002 ; Vol. 89, No. 4. pp. 395-404.
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