Recent advances in derivation of functional hepatocytes from placental stem cells

Melania Lo Iacono, Rita Anzalone, Simona Corrao, Tiziana Gorgun, Carla Loreto, Salvatore Sansalone, Maria Sergio, Marcello Cimador, Mario Giuffrè, Felicia Farina, Giampiero La Rocca

Research output: Contribution to journalReview article

Abstract

End-stage liver diseases are one of the leading causes of death in the world. Often orthotopic liver transplantation represents the final therapeutic choice. The limits of this approach are the scarcity of donor livers available, and the many side effects related to the administration of immune suppressants to the patients. Cellular therapy for liver diseases is increasingly being viewed as a promising strategy to provide hepatocytes to replenish the parenchymal cells of the organ. This technique suffers of some important limitations, such as the difficulty in isolating sufficient cell numbers (e.g. when adult or foetal hepatocytes are used for transplantation), the limited viability of isolated hepatocytes and, when applicable, the limited differentiation of stem cells (when hepatocyte-like cells are derived from hepatic or extra-hepatic progenitor populations). In recent years, perinatal stem cells have been proposed as reliable cellular populations which may be successfully used to derive hepatocyte-like cells. These cells feature key advantages over other adult stem cells: may be easily sourced from the tissues of origin, can be expanded ex vivo to obtain high cell numbers, may be differentiated towards hepatocyte-like cells. In addition, these cells feature relevant immunomodulatory and anti-inflammatory activities, and their sourcing is not limited by ethical concerns In the present review we analyze the molecular basis of hepatocyte biology and development, and discuss the recent advances in deriving hapatocyte-like cells from perinatal stem cells. Very recent papers on mesenchymal stem cells derived from bone marrow and adipose tissues are also comparatively discussed as prototypes of the use of adult extrahepatic stem cells. In our opinion, perinatal stem cells do represent a promising tool for liver regenerative medicine, and recent research reports further strengthened this perception and fostered further efforts by multiple research groups worldwide.

Original languageEnglish (US)
Pages (from-to)12-25
Number of pages14
JournalOpen Tissue Engineering and Regenerative Medicine Journal
Volume6
Issue number1
DOIs
StatePublished - Nov 26 2013
Externally publishedYes

Fingerprint

Hepatocytes
Stem Cells
Adult Stem Cells
Liver
Cell Count
End Stage Liver Disease
Regenerative Medicine
Mesenchymal Stromal Cells
Liver Transplantation
Population
Adipose Tissue
Liver Diseases
Cause of Death
Anti-Inflammatory Agents
Transplantation
Bone Marrow
Tissue Donors
Bone and Bones
Therapeutics
Research

Keywords

  • Amniotic fluid
  • Amniotic membrane
  • Fibrosis
  • Functional assays
  • Hepatocyte differentiation
  • Immune modulation
  • Inflammation
  • Mesenchymal stem cells
  • Regenerative medicine
  • Tissue repair
  • Umbilical cord
  • Wharton's jelly

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

Cite this

Recent advances in derivation of functional hepatocytes from placental stem cells. / Lo Iacono, Melania; Anzalone, Rita; Corrao, Simona; Gorgun, Tiziana; Loreto, Carla; Sansalone, Salvatore; Sergio, Maria; Cimador, Marcello; Giuffrè, Mario; Farina, Felicia; La Rocca, Giampiero.

In: Open Tissue Engineering and Regenerative Medicine Journal, Vol. 6, No. 1, 26.11.2013, p. 12-25.

Research output: Contribution to journalReview article

Lo Iacono, M, Anzalone, R, Corrao, S, Gorgun, T, Loreto, C, Sansalone, S, Sergio, M, Cimador, M, Giuffrè, M, Farina, F & La Rocca, G 2013, 'Recent advances in derivation of functional hepatocytes from placental stem cells', Open Tissue Engineering and Regenerative Medicine Journal, vol. 6, no. 1, pp. 12-25. https://doi.org/10.2174/1875043501306010012
Lo Iacono, Melania ; Anzalone, Rita ; Corrao, Simona ; Gorgun, Tiziana ; Loreto, Carla ; Sansalone, Salvatore ; Sergio, Maria ; Cimador, Marcello ; Giuffrè, Mario ; Farina, Felicia ; La Rocca, Giampiero. / Recent advances in derivation of functional hepatocytes from placental stem cells. In: Open Tissue Engineering and Regenerative Medicine Journal. 2013 ; Vol. 6, No. 1. pp. 12-25.
@article{585ca8d260e5463aab1aa5f55a4c1b39,
title = "Recent advances in derivation of functional hepatocytes from placental stem cells",
abstract = "End-stage liver diseases are one of the leading causes of death in the world. Often orthotopic liver transplantation represents the final therapeutic choice. The limits of this approach are the scarcity of donor livers available, and the many side effects related to the administration of immune suppressants to the patients. Cellular therapy for liver diseases is increasingly being viewed as a promising strategy to provide hepatocytes to replenish the parenchymal cells of the organ. This technique suffers of some important limitations, such as the difficulty in isolating sufficient cell numbers (e.g. when adult or foetal hepatocytes are used for transplantation), the limited viability of isolated hepatocytes and, when applicable, the limited differentiation of stem cells (when hepatocyte-like cells are derived from hepatic or extra-hepatic progenitor populations). In recent years, perinatal stem cells have been proposed as reliable cellular populations which may be successfully used to derive hepatocyte-like cells. These cells feature key advantages over other adult stem cells: may be easily sourced from the tissues of origin, can be expanded ex vivo to obtain high cell numbers, may be differentiated towards hepatocyte-like cells. In addition, these cells feature relevant immunomodulatory and anti-inflammatory activities, and their sourcing is not limited by ethical concerns In the present review we analyze the molecular basis of hepatocyte biology and development, and discuss the recent advances in deriving hapatocyte-like cells from perinatal stem cells. Very recent papers on mesenchymal stem cells derived from bone marrow and adipose tissues are also comparatively discussed as prototypes of the use of adult extrahepatic stem cells. In our opinion, perinatal stem cells do represent a promising tool for liver regenerative medicine, and recent research reports further strengthened this perception and fostered further efforts by multiple research groups worldwide.",
keywords = "Amniotic fluid, Amniotic membrane, Fibrosis, Functional assays, Hepatocyte differentiation, Immune modulation, Inflammation, Mesenchymal stem cells, Regenerative medicine, Tissue repair, Umbilical cord, Wharton's jelly",
author = "{Lo Iacono}, Melania and Rita Anzalone and Simona Corrao and Tiziana Gorgun and Carla Loreto and Salvatore Sansalone and Maria Sergio and Marcello Cimador and Mario Giuffr{\`e} and Felicia Farina and {La Rocca}, Giampiero",
year = "2013",
month = "11",
day = "26",
doi = "10.2174/1875043501306010012",
language = "English (US)",
volume = "6",
pages = "12--25",
journal = "Open Tissue Engineering and Regenerative Medicine Journal",
issn = "1875-0435",
publisher = "Bentham Science Publishers B.V.",
number = "1",

}

TY - JOUR

T1 - Recent advances in derivation of functional hepatocytes from placental stem cells

AU - Lo Iacono, Melania

AU - Anzalone, Rita

AU - Corrao, Simona

AU - Gorgun, Tiziana

AU - Loreto, Carla

AU - Sansalone, Salvatore

AU - Sergio, Maria

AU - Cimador, Marcello

AU - Giuffrè, Mario

AU - Farina, Felicia

AU - La Rocca, Giampiero

PY - 2013/11/26

Y1 - 2013/11/26

N2 - End-stage liver diseases are one of the leading causes of death in the world. Often orthotopic liver transplantation represents the final therapeutic choice. The limits of this approach are the scarcity of donor livers available, and the many side effects related to the administration of immune suppressants to the patients. Cellular therapy for liver diseases is increasingly being viewed as a promising strategy to provide hepatocytes to replenish the parenchymal cells of the organ. This technique suffers of some important limitations, such as the difficulty in isolating sufficient cell numbers (e.g. when adult or foetal hepatocytes are used for transplantation), the limited viability of isolated hepatocytes and, when applicable, the limited differentiation of stem cells (when hepatocyte-like cells are derived from hepatic or extra-hepatic progenitor populations). In recent years, perinatal stem cells have been proposed as reliable cellular populations which may be successfully used to derive hepatocyte-like cells. These cells feature key advantages over other adult stem cells: may be easily sourced from the tissues of origin, can be expanded ex vivo to obtain high cell numbers, may be differentiated towards hepatocyte-like cells. In addition, these cells feature relevant immunomodulatory and anti-inflammatory activities, and their sourcing is not limited by ethical concerns In the present review we analyze the molecular basis of hepatocyte biology and development, and discuss the recent advances in deriving hapatocyte-like cells from perinatal stem cells. Very recent papers on mesenchymal stem cells derived from bone marrow and adipose tissues are also comparatively discussed as prototypes of the use of adult extrahepatic stem cells. In our opinion, perinatal stem cells do represent a promising tool for liver regenerative medicine, and recent research reports further strengthened this perception and fostered further efforts by multiple research groups worldwide.

AB - End-stage liver diseases are one of the leading causes of death in the world. Often orthotopic liver transplantation represents the final therapeutic choice. The limits of this approach are the scarcity of donor livers available, and the many side effects related to the administration of immune suppressants to the patients. Cellular therapy for liver diseases is increasingly being viewed as a promising strategy to provide hepatocytes to replenish the parenchymal cells of the organ. This technique suffers of some important limitations, such as the difficulty in isolating sufficient cell numbers (e.g. when adult or foetal hepatocytes are used for transplantation), the limited viability of isolated hepatocytes and, when applicable, the limited differentiation of stem cells (when hepatocyte-like cells are derived from hepatic or extra-hepatic progenitor populations). In recent years, perinatal stem cells have been proposed as reliable cellular populations which may be successfully used to derive hepatocyte-like cells. These cells feature key advantages over other adult stem cells: may be easily sourced from the tissues of origin, can be expanded ex vivo to obtain high cell numbers, may be differentiated towards hepatocyte-like cells. In addition, these cells feature relevant immunomodulatory and anti-inflammatory activities, and their sourcing is not limited by ethical concerns In the present review we analyze the molecular basis of hepatocyte biology and development, and discuss the recent advances in deriving hapatocyte-like cells from perinatal stem cells. Very recent papers on mesenchymal stem cells derived from bone marrow and adipose tissues are also comparatively discussed as prototypes of the use of adult extrahepatic stem cells. In our opinion, perinatal stem cells do represent a promising tool for liver regenerative medicine, and recent research reports further strengthened this perception and fostered further efforts by multiple research groups worldwide.

KW - Amniotic fluid

KW - Amniotic membrane

KW - Fibrosis

KW - Functional assays

KW - Hepatocyte differentiation

KW - Immune modulation

KW - Inflammation

KW - Mesenchymal stem cells

KW - Regenerative medicine

KW - Tissue repair

KW - Umbilical cord

KW - Wharton's jelly

UR - http://www.scopus.com/inward/record.url?scp=84888028757&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84888028757&partnerID=8YFLogxK

U2 - 10.2174/1875043501306010012

DO - 10.2174/1875043501306010012

M3 - Review article

VL - 6

SP - 12

EP - 25

JO - Open Tissue Engineering and Regenerative Medicine Journal

JF - Open Tissue Engineering and Regenerative Medicine Journal

SN - 1875-0435

IS - 1

ER -