Recent advances in the measurement of adiponectin isoform distribution

Faidon Magkos, Labros S. Sidossis

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

PURPOSE OF REVIEW: Adiponectin has potent antidiabetic and antiatherosclerotic actions. Recent studies in animals and humans suggest that the high-molecular weight adiponectin complex, consisting of many adiponectin monomers, is the biologically active form of the peptide. This article will present recent methodological approaches for analyzing adiponectin isoform distribution. RECENT FINDINGS: A handful of methods have been used for the isolation and measurement of high-molecular weight adiponectin, based on velocity gradient centrifugation, gel filtration chromatography and polyacrylamide gel electrophoresis. Recently, two novel sandwich enzyme-linked immuno-sorbent assays have been developed. The first makes use of antibodies raised against human high-molecular weight adiponectin and thus allows for the specific determination of high-molecular weight adiponectin in plasma. The second and more versatile enzyme-linked immuno-sorbent assay system enables the measurement of all adiponectin isoforms (i.e. low, middle and high-molecular weight) by means of selective digestion by proteases. SUMMARY: The development and commercial availability of novel enzyme-linked immuno-sorbent assay kits enables the easy and rapid measurement of high-molecular weight adiponectin in both research and clinical practice and will undoubtedly advance further our understanding of the role of adiponectin in health and disease.

Original languageEnglish (US)
Pages (from-to)571-575
Number of pages5
JournalCurrent Opinion in Clinical Nutrition and Metabolic Care
Volume10
Issue number5
DOIs
StatePublished - Sep 2007
Externally publishedYes

Fingerprint

adiponectin
Adiponectin
Protein Isoforms
Molecular Weight
molecular weight
enzyme-linked immunosorbent assay
Enzymes
hypoglycemic agents
sandwiches
Centrifugation
Hypoglycemic Agents
centrifugation
Gel Chromatography
polyacrylamide gel electrophoresis
Polyacrylamide Gel Electrophoresis
Digestion
Peptide Hydrolases
proteinases
digestion
peptides

Keywords

  • Acrp30
  • Adipokines
  • AdipoQ
  • ELISA

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology
  • Food Science
  • Endocrinology, Diabetes and Metabolism

Cite this

Recent advances in the measurement of adiponectin isoform distribution. / Magkos, Faidon; Sidossis, Labros S.

In: Current Opinion in Clinical Nutrition and Metabolic Care, Vol. 10, No. 5, 09.2007, p. 571-575.

Research output: Contribution to journalArticle

@article{029f7e6e83a9483fb5fb46d2281d69c1,
title = "Recent advances in the measurement of adiponectin isoform distribution",
abstract = "PURPOSE OF REVIEW: Adiponectin has potent antidiabetic and antiatherosclerotic actions. Recent studies in animals and humans suggest that the high-molecular weight adiponectin complex, consisting of many adiponectin monomers, is the biologically active form of the peptide. This article will present recent methodological approaches for analyzing adiponectin isoform distribution. RECENT FINDINGS: A handful of methods have been used for the isolation and measurement of high-molecular weight adiponectin, based on velocity gradient centrifugation, gel filtration chromatography and polyacrylamide gel electrophoresis. Recently, two novel sandwich enzyme-linked immuno-sorbent assays have been developed. The first makes use of antibodies raised against human high-molecular weight adiponectin and thus allows for the specific determination of high-molecular weight adiponectin in plasma. The second and more versatile enzyme-linked immuno-sorbent assay system enables the measurement of all adiponectin isoforms (i.e. low, middle and high-molecular weight) by means of selective digestion by proteases. SUMMARY: The development and commercial availability of novel enzyme-linked immuno-sorbent assay kits enables the easy and rapid measurement of high-molecular weight adiponectin in both research and clinical practice and will undoubtedly advance further our understanding of the role of adiponectin in health and disease.",
keywords = "Acrp30, Adipokines, AdipoQ, ELISA",
author = "Faidon Magkos and Sidossis, {Labros S.}",
year = "2007",
month = "9",
doi = "10.1097/MCO.0b013e3282bf6ea8",
language = "English (US)",
volume = "10",
pages = "571--575",
journal = "Current Opinion in Clinical Nutrition and Metabolic Care",
issn = "1363-1950",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Recent advances in the measurement of adiponectin isoform distribution

AU - Magkos, Faidon

AU - Sidossis, Labros S.

PY - 2007/9

Y1 - 2007/9

N2 - PURPOSE OF REVIEW: Adiponectin has potent antidiabetic and antiatherosclerotic actions. Recent studies in animals and humans suggest that the high-molecular weight adiponectin complex, consisting of many adiponectin monomers, is the biologically active form of the peptide. This article will present recent methodological approaches for analyzing adiponectin isoform distribution. RECENT FINDINGS: A handful of methods have been used for the isolation and measurement of high-molecular weight adiponectin, based on velocity gradient centrifugation, gel filtration chromatography and polyacrylamide gel electrophoresis. Recently, two novel sandwich enzyme-linked immuno-sorbent assays have been developed. The first makes use of antibodies raised against human high-molecular weight adiponectin and thus allows for the specific determination of high-molecular weight adiponectin in plasma. The second and more versatile enzyme-linked immuno-sorbent assay system enables the measurement of all adiponectin isoforms (i.e. low, middle and high-molecular weight) by means of selective digestion by proteases. SUMMARY: The development and commercial availability of novel enzyme-linked immuno-sorbent assay kits enables the easy and rapid measurement of high-molecular weight adiponectin in both research and clinical practice and will undoubtedly advance further our understanding of the role of adiponectin in health and disease.

AB - PURPOSE OF REVIEW: Adiponectin has potent antidiabetic and antiatherosclerotic actions. Recent studies in animals and humans suggest that the high-molecular weight adiponectin complex, consisting of many adiponectin monomers, is the biologically active form of the peptide. This article will present recent methodological approaches for analyzing adiponectin isoform distribution. RECENT FINDINGS: A handful of methods have been used for the isolation and measurement of high-molecular weight adiponectin, based on velocity gradient centrifugation, gel filtration chromatography and polyacrylamide gel electrophoresis. Recently, two novel sandwich enzyme-linked immuno-sorbent assays have been developed. The first makes use of antibodies raised against human high-molecular weight adiponectin and thus allows for the specific determination of high-molecular weight adiponectin in plasma. The second and more versatile enzyme-linked immuno-sorbent assay system enables the measurement of all adiponectin isoforms (i.e. low, middle and high-molecular weight) by means of selective digestion by proteases. SUMMARY: The development and commercial availability of novel enzyme-linked immuno-sorbent assay kits enables the easy and rapid measurement of high-molecular weight adiponectin in both research and clinical practice and will undoubtedly advance further our understanding of the role of adiponectin in health and disease.

KW - Acrp30

KW - Adipokines

KW - AdipoQ

KW - ELISA

UR - http://www.scopus.com/inward/record.url?scp=34547911845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547911845&partnerID=8YFLogxK

U2 - 10.1097/MCO.0b013e3282bf6ea8

DO - 10.1097/MCO.0b013e3282bf6ea8

M3 - Article

C2 - 17693739

AN - SCOPUS:34547911845

VL - 10

SP - 571

EP - 575

JO - Current Opinion in Clinical Nutrition and Metabolic Care

JF - Current Opinion in Clinical Nutrition and Metabolic Care

SN - 1363-1950

IS - 5

ER -