Recognition of disparate HA and NS1 peptides by an H-2Kd-restricted, influenza specific CTL clone

Kuwano Koichi, Victor Reyes, Robert E. Humphreys, Francis A. Ennis

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

CTLs (CD8+) are known to recognize exogenous peptide in the context of class I MHC molecules. We observed that an influenza subtype H1 and H2 cross-reactive CTL clone B7, which was stimulated by a fusion protein containing a portion of HA2 subunit of A/PR/8 virus HA, recognized a synthetic peptide (residues 515-526) of the HA2 subunit of A/PR/8 virus strain. This CTL clone also recognized a structurally disparate NS1 peptide 50-68 of the same A/PR/8 virus. We examined the recognition of the NS1 peptide 50-68 and the HA peptide 515-526 by the subcloned CTL clone, B7-B7. Cold target inhibition experiments showed that the recognition of the HA peptide by the CTL clone B7-B7 could be competed by NS1 peptide-treated target cells and vice versa. The recognition of both NS1 peptide and HA peptide by the CTL clone B7-B7 was restricted by the same allele, H2Kd. In addition, this NS1 peptide requires approximately a 600-fold higher concn for optimal CTL recognition than did the HA peptide. We conclude that the TCR on clone B7-B7 recognizes the HA peptide or the NS1 peptide as comparable complexes with the same class I MHC molecules, although there is no obvious homology in the primary sequences of HA 515-526 and NS1 50-68 peptides. CTLs elicited with certain antigens appear to recognize distinctively different antigens complexed to the same presenting MHC molecule.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalMolecular Immunology
Volume28
Issue number1-2
DOIs
StatePublished - 1991
Externally publishedYes

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Human Influenza
Clone Cells
Peptides
Viruses
Antigens
Alleles

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Recognition of disparate HA and NS1 peptides by an H-2Kd-restricted, influenza specific CTL clone. / Koichi, Kuwano; Reyes, Victor; Humphreys, Robert E.; Ennis, Francis A.

In: Molecular Immunology, Vol. 28, No. 1-2, 1991, p. 1-7.

Research output: Contribution to journalArticle

Koichi, Kuwano ; Reyes, Victor ; Humphreys, Robert E. ; Ennis, Francis A. / Recognition of disparate HA and NS1 peptides by an H-2Kd-restricted, influenza specific CTL clone. In: Molecular Immunology. 1991 ; Vol. 28, No. 1-2. pp. 1-7.
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