Recognition of type 1 chain oligosaccharides and lacto-series glycolipids by an antibody to human secretory component

Haini Yu, John M. Sipes, JoAnne Cashel, Mary Ann Bakos, Randall M. Goldblum, David D. Roberts

Research output: Contribution to journalArticle

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Abstract

Binding of the mouse IgM antibody 6C4 is lost after treatment of human free secretory component with peptide N-glycosidase F (Bakos et al. (1991) J. Immunol. 146, 162-168) or periodate, suggesting that asparagine-linked oligosaccharides contain the epitope recognized by this antibody. Inhibition of antibody binding to free secretory component by milk oligosaccharides established that lacto-N-tetraose is the minimum structure recognized by the antibody, but larger oligosaccharides with terminal Galβ1-3GlcNAc sequences bind with much higher affinity. Antibody binding is enhanced by substitution with the Lewis Fucα1-4 and is inhibited by Fucα1-2Ga1 substitution. Free secretory component, however, does not bind other antibodies that recognize Lea or Leb oligosaccharides, and binding is lost after digestion with a β- galactosidase that cleaves Galβ1-3 linkages but not after digestion with α- L-fucosidase. Therefore, the major epitope recognized by 6C4 on free secretory component is probably not an asparagine-linked Lea oligosaccharide. The antibody also binds to human milk lactoferrin, some human mucins, and lacto-series glycolipids including III4αFuc- lactotetraosyl ceramide and lactotetraosyl ceramide. Based on affinity chromatography of oligosaccharities released from free secretory component, the epitope recognized by antibody 6C4 is present on approximately 3.5% of the asparagine-linked oligosaccharides.

Original languageEnglish
Pages (from-to)299-305
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume322
Issue number2
DOIs
StatePublished - 1995

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Secretory Component
Glycolipids
Oligosaccharides
Antibodies
Asparagine
Epitopes
Ceramides
Digestion
Substitution reactions
Galactosidases
alpha-L-Fucosidase
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Affinity chromatography
Lactoferrin
Mucins
Human Milk
Affinity Chromatography
Immunoglobulin M
Milk

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Recognition of type 1 chain oligosaccharides and lacto-series glycolipids by an antibody to human secretory component. / Yu, Haini; Sipes, John M.; Cashel, JoAnne; Bakos, Mary Ann; Goldblum, Randall M.; Roberts, David D.

In: Archives of Biochemistry and Biophysics, Vol. 322, No. 2, 1995, p. 299-305.

Research output: Contribution to journalArticle

Yu, Haini ; Sipes, John M. ; Cashel, JoAnne ; Bakos, Mary Ann ; Goldblum, Randall M. ; Roberts, David D. / Recognition of type 1 chain oligosaccharides and lacto-series glycolipids by an antibody to human secretory component. In: Archives of Biochemistry and Biophysics. 1995 ; Vol. 322, No. 2. pp. 299-305.
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