Recombinant alphaviruses are safe and useful serological diagnostic tools

Haolin Ni, Nadezhda E. Yun, Michele A. Zacks, Scott Weaver, Robert B. Tesh, Amelia P. Travassos Da Rosa, Ann M. Powers, Ilya Frolov, Slobodan Paessler

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Serological assays for diagnosis of Venezuelan equine encephalitis virus (VEEV) currently require biosafety level 3 facilities and select agent certification to produce antigens, reference sera, or viral stocks. Rapid identification of VEEV infection is required to respond to human and equine outbreaks of encephalitis caused by that virus and can be useful for epidemiologic surveillance. Alphavirus (Sindbis)-based recombinant viruses that express VEEV structural proteins are attenuated in animal models, thus representing an alternative to the handling of virulent infectious virus. Virus and viral antigens from recombinant Sindbis/VEE constructs engineered to express structural proteins from multiple VEEV subtypes were evaluated as diagnostic reagents in VEEV-specific serological assays, e.g., plaque reduction neutralization test (PRNT), hemagglutination inhibition (HI) assay, and complement fixation (CF) test. Chimeric viruses were produced efficiently in cell culture and were as effective as the parental virus for identifying infection of humans, horses, and rodents in these serological assays.

Original languageEnglish (US)
Pages (from-to)774-781
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume76
Issue number4
StatePublished - Apr 2007

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Venezuelan Equine Encephalitis Viruses
Alphavirus
Viruses
Virus Diseases
Equine Encephalomyelitis
Encephalitis Viruses
Epidemiological Monitoring
Viral Structural Proteins
Complement Fixation Tests
Neutralization Tests
Viral Antigens
Certification
Hemagglutination
Horses
Disease Outbreaks
Rodentia
Animal Models
Cell Culture Techniques
Antigens
Serum

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Recombinant alphaviruses are safe and useful serological diagnostic tools. / Ni, Haolin; Yun, Nadezhda E.; Zacks, Michele A.; Weaver, Scott; Tesh, Robert B.; Travassos Da Rosa, Amelia P.; Powers, Ann M.; Frolov, Ilya; Paessler, Slobodan.

In: American Journal of Tropical Medicine and Hygiene, Vol. 76, No. 4, 04.2007, p. 774-781.

Research output: Contribution to journalArticle

Ni, H, Yun, NE, Zacks, MA, Weaver, S, Tesh, RB, Travassos Da Rosa, AP, Powers, AM, Frolov, I & Paessler, S 2007, 'Recombinant alphaviruses are safe and useful serological diagnostic tools', American Journal of Tropical Medicine and Hygiene, vol. 76, no. 4, pp. 774-781.
Ni H, Yun NE, Zacks MA, Weaver S, Tesh RB, Travassos Da Rosa AP et al. Recombinant alphaviruses are safe and useful serological diagnostic tools. American Journal of Tropical Medicine and Hygiene. 2007 Apr;76(4):774-781.
Ni, Haolin ; Yun, Nadezhda E. ; Zacks, Michele A. ; Weaver, Scott ; Tesh, Robert B. ; Travassos Da Rosa, Amelia P. ; Powers, Ann M. ; Frolov, Ilya ; Paessler, Slobodan. / Recombinant alphaviruses are safe and useful serological diagnostic tools. In: American Journal of Tropical Medicine and Hygiene. 2007 ; Vol. 76, No. 4. pp. 774-781.
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