Recombinant alphaviruses are safe and useful serological diagnostic tools

Haolin Ni, Nadezhda E. Yun, Michele A. Zacks, Scott C. Weaver, Robert B. Tesh, Amelia P. Travassos Da Rosa, Ann M. Powers, Ilya Frolov, Slobodan Paessler

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Serological assays for diagnosis of Venezuelan equine encephalitis virus (VEEV) currently require biosafety level 3 facilities and select agent certification to produce antigens, reference sera, or viral stocks. Rapid identification of VEEV infection is required to respond to human and equine outbreaks of encephalitis caused by that virus and can be useful for epidemiologic surveillance. Alphavirus (Sindbis)-based recombinant viruses that express VEEV structural proteins are attenuated in animal models, thus representing an alternative to the handling of virulent infectious virus. Virus and viral antigens from recombinant Sindbis/VEE constructs engineered to express structural proteins from multiple VEEV subtypes were evaluated as diagnostic reagents in VEEV-specific serological assays, e.g., plaque reduction neutralization test (PRNT), hemagglutination inhibition (HI) assay, and complement fixation (CF) test. Chimeric viruses were produced efficiently in cell culture and were as effective as the parental virus for identifying infection of humans, horses, and rodents in these serological assays.

Original languageEnglish (US)
Pages (from-to)774-781
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume76
Issue number4
StatePublished - Apr 1 2007

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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    Ni, H., Yun, N. E., Zacks, M. A., Weaver, S. C., Tesh, R. B., Travassos Da Rosa, A. P., Powers, A. M., Frolov, I., & Paessler, S. (2007). Recombinant alphaviruses are safe and useful serological diagnostic tools. American Journal of Tropical Medicine and Hygiene, 76(4), 774-781.